EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.56 | AgNO3 | 1 mM, 61% inhibition | Escherichia coli | |
2.5.1.56 | Cu2+ | 1 mM CuCl2, 29% inhibition | Escherichia coli | |
2.5.1.56 | HgCl2 | at 0.01 mM 85% inhibition, at 0.1 mM complete inhibition | Escherichia coli | |
2.5.1.56 | hydroxylamine | 1 mM, 23% inhibition | Escherichia coli | |
2.5.1.56 | Iodine | 1 mM, 30% inhibition | Escherichia coli | |
2.5.1.56 | N-bromosuccinimide | 1 mM, 24% inhibition | Escherichia coli | |
2.5.1.56 | PCMB | 1 mM, complete inhibition | Escherichia coli | |
2.5.1.56 | Semicarbazide | 1 mM, 20% inhibition | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.56 | 0.04 | - |
phosphoenolpyruvate | pH 8.2, 37°C | Escherichia coli | |
2.5.1.56 | 0.042 | - |
phosphoenolpyruvate | pH 8.2, 37°C | Escherichia coli | |
2.5.1.56 | 5.6 | - |
N-acetyl-D-mannosamine | pH 8.2, 37°C | Escherichia coli | |
2.5.1.56 | 6.25 | - |
N-acetyl-D-mannosamine | pH 8.2, 37°C | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.56 | additional information | no metal ion requirement | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 52000 | - |
2 * 52000, SDS-PAGE | Escherichia coli |
2.5.1.56 | 106000 | - |
gel filtration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.56 | Escherichia coli | - |
- |
- |
2.5.1.56 | Escherichia coli K1-M12 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.56 | - |
Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 487 | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.56 | N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | - |
Escherichia coli | N-acetylneuraminate + phosphate | - |
? | |
2.5.1.56 | N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | - |
Escherichia coli K1-M12 | N-acetylneuraminate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.56 | dimer | 2 * 52000, SDS-PAGE | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 35 | - |
- |
Escherichia coli |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 20 | 60 | about 60% of maximal activity at 20°C and at 60°C | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 30 | - |
pH 7.5, 30 min, stable up to | Escherichia coli |
2.5.1.56 | 40 | - |
pH 7.5, 30 min, about 60% loss of activity | Escherichia coli |
2.5.1.56 | 50 | - |
pH 7.5, 30 min, about 80% loss of activity | Escherichia coli |
2.5.1.56 | 60 | - |
pH 7.5, 30 min, complete loss of activity | Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 4 | 12 | pH 4.0: about 50% of maximal activity, pH 12.0: about 40% of maximal activity | Escherichia coli |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.56 | 4 | - |
25°C, 1 h, about 50% loss of activity | Escherichia coli |
2.5.1.56 | 5 | - |
25°C, 1 h, about 40% loss of activity | Escherichia coli |
2.5.1.56 | 6 | - |
25°C, 1 h, about 25% loss of activity | Escherichia coli |
2.5.1.56 | 7 | 10 | 25°C, 1 h, stable | Escherichia coli |
2.5.1.56 | 11 | - |
25°C, 1 h, about 40% loss of activity | Escherichia coli |
2.5.1.56 | 12 | - |
25°C, 1 h, about 60% loss of activity | Escherichia coli |