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Literature summary extracted from

  • Komaki, E.; Ohta, Y.; Tsukada, Y.
    Purification and characterization of N-acetylneuraminate synthase from Escherichia coli K1-M12 (1997), Biosci. Biotechnol. Biochem., 61, 2046-2050.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.56 AgNO3 1 mM, 61% inhibition Escherichia coli
2.5.1.56 Cu2+ 1 mM CuCl2, 29% inhibition Escherichia coli
2.5.1.56 HgCl2 at 0.01 mM 85% inhibition, at 0.1 mM complete inhibition Escherichia coli
2.5.1.56 hydroxylamine 1 mM, 23% inhibition Escherichia coli
2.5.1.56 Iodine 1 mM, 30% inhibition Escherichia coli
2.5.1.56 N-bromosuccinimide 1 mM, 24% inhibition Escherichia coli
2.5.1.56 PCMB 1 mM, complete inhibition Escherichia coli
2.5.1.56 Semicarbazide 1 mM, 20% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.56 0.04
-
phosphoenolpyruvate pH 8.2, 37°C Escherichia coli
2.5.1.56 0.042
-
phosphoenolpyruvate pH 8.2, 37°C Escherichia coli
2.5.1.56 5.6
-
N-acetyl-D-mannosamine pH 8.2, 37°C Escherichia coli
2.5.1.56 6.25
-
N-acetyl-D-mannosamine pH 8.2, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.56 additional information no metal ion requirement Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.56 52000
-
2 * 52000, SDS-PAGE Escherichia coli
2.5.1.56 106000
-
gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.56 Escherichia coli
-
-
-
2.5.1.56 Escherichia coli K1-M12
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.56
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.56 487
-
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.56 N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O
-
Escherichia coli N-acetylneuraminate + phosphate
-
?
2.5.1.56 N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O
-
Escherichia coli K1-M12 N-acetylneuraminate + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.56 dimer 2 * 52000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.56 35
-
-
Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.56 20 60 about 60% of maximal activity at 20°C and at 60°C Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.56 30
-
pH 7.5, 30 min, stable up to Escherichia coli
2.5.1.56 40
-
pH 7.5, 30 min, about 60% loss of activity Escherichia coli
2.5.1.56 50
-
pH 7.5, 30 min, about 80% loss of activity Escherichia coli
2.5.1.56 60
-
pH 7.5, 30 min, complete loss of activity Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.56 4 12 pH 4.0: about 50% of maximal activity, pH 12.0: about 40% of maximal activity Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.56 4
-
25°C, 1 h, about 50% loss of activity Escherichia coli
2.5.1.56 5
-
25°C, 1 h, about 40% loss of activity Escherichia coli
2.5.1.56 6
-
25°C, 1 h, about 25% loss of activity Escherichia coli
2.5.1.56 7 10 25°C, 1 h, stable Escherichia coli
2.5.1.56 11
-
25°C, 1 h, about 40% loss of activity Escherichia coli
2.5.1.56 12
-
25°C, 1 h, about 60% loss of activity Escherichia coli