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Literature summary extracted from

  • Williams, J.W.; Northrop, D.B.
    Purification and properties of gentamicin acetyltransferase I (1976), Biochemistry, 15, 125-131.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.60 additional information N-methylmaleimide and DTT have no effect on activity Escherichia coli

General Stability

EC Number General Stability Organism
2.3.1.60 lyophilization, stable to Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.60 gentamicin
-
 Escherichia coli
2.3.1.60 gentamicin C1a substrate inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.60 0.0003 0.0005 gentamicin C1a
-
 Escherichia coli
2.3.1.60 0.0039 0.0048 acetyl-CoA
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.60 cytoplasm
-
 Escherichia coli 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.60 17000
-
 4 * 17000, SDS-PAGE Escherichia coli
2.3.1.60 63000
-
 equilibrium sedimentation centrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.60 Escherichia coli
-
 containing R-factor JR88
-
2.3.1.60 Escherichia coli C600
-
 containing R-factor JR88
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.60
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.60 3.8
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.60 20°C, lyophilized, up to 1 month Escherichia coli
2.3.1.60 frozen, redissolved ammonium sulfate precipitate, at least 1 month Escherichia coli
2.3.1.60 refrigerated, lyophilized, up to 1 month Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.60 acetyl-CoA + gentamicin C1a
-
 Escherichia coli CoA + N3'-acetylgentamicin C1a
-
 ?
2.3.1.60 acetyl-CoA + gentamicin C1a
-
 Escherichia coli C600 CoA + N3'-acetylgentamicin C1a
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.60 tetramer 4 * 17000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.60 25
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.60 3.57
-
 N3'-acetylgentamicin C1a
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.60 7.7 8
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.60 5.5 8.5
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.60 4
-
 irreversible inactivation Escherichia coli
2.3.1.60 4.5
-
 no activity, restorable by neutralization Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.60 0.16
-
 gentamicin
-
 Escherichia coli