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Literature summary extracted from

  • Halliday, J.A.W.; Franks, A.H.; Ramsdale, T.E.; Martin, R.; Palant, E.
    A rapid, semi-automated method for detection of Galbeta1-4GlcNAc alpha2,6-sialyltransferase (EC 2.4.99.1) activity using the lectin Sambucus nigra agglutinin (2001), Glycobiology, 11, 557-564.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.1 Triton X-100
-
 Homo sapiens
2.4.3.1 Triton X-100
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 0.035
-
 CMP-N-acetylneuraminate
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Homo sapiens
-
 recombinant
-
2.4.3.1 Rattus norvegicus
-
 commercial product
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 commercial preparation
-
 Rattus norvegicus
-
2.4.3.1 liver
-
 Homo sapiens
-
2.4.3.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.1 additional information
-
 assay method development, biosensor-based method using immobilized Sambucus nigra agglutinin Homo sapiens
2.4.3.1 additional information
-
 assay method development, biosensor-based method using immobilized Sambucus nigra agglutinin Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + asialo-fetuin
-
 Homo sapiens CMP + fetuin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + asialo-fetuin
-
 Rattus norvegicus CMP + fetuin
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 37
-
 assay at Homo sapiens
2.4.3.1 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.1 6.5
-
 assay at Homo sapiens
2.4.3.1 6.5
-
 assay at Rattus norvegicus