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Literature summary extracted from

  • Hori, H.; Yamazaki, N.; Matsumoto, T.; Watanabe, Y.I.; Ueda, T.; Nishikawa, K.; Kumagai, I.; Watanabe, K.
    Substrate recognition of tRNA (guanosine-2'-)-methyltransferase from Thermus thermophilus HB27 (1998), J. Biol. Chem., 273, 25721-25727.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.34 DELTAD-arm tRNAPhe variant from yeast with a modification in the D-stem, inhibition Thermus thermophilus
2.1.1.34 G18A tRNAPhe variant from yeast with an exchange of G to A at position 18, inhibition Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.34 additional information
-
 additional information overview: Km of 28 tRNAPhe variants from yeast Thermus thermophilus
2.1.1.34 0.000005
-
 guanosine18 in Escherichia coli tRNA-Met
-
 Thermus thermophilus
2.1.1.34 0.000005
-
 guanosine18 in yeast tRNA-Phe below Thermus thermophilus
2.1.1.34 0.000006
-
 Escherichia coli tRNA-Ile
-
 Thermus thermophilus
2.1.1.34 0.00001
-
 guanosine18 in Escherichia coli tRNA-Phe
-
 Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.34 Thermus thermophilus
-
-
-
2.1.1.34 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA substrate recognition sites and mechanism Thermus thermophilus
a
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA G18, G19 and the D-stem of the tRNA are required for Gm-methylase recognition Thermus thermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.34 additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus ?
-
 ?
2.1.1.34 additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
-
 ?
2.1.1.34 S-adenosyl-L-methionine + Escherichia coli tRNA-Met
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Met
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in Escherichia coli tRNA-Ile
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Ile
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in Escherichia coli tRNA-Phe
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Phe
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA substrate tRNAs from E. coli, yeast, Bacillus subtilis Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA substrate tRNAs from E. coli, yeast, Bacillus subtilis Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in yeast tRNA-Phe
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNA-Phe
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in yeast tRNA-Phe
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNA-Phe
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.34 65
-
 assay at Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.34 0.00079
-
 G18A
-
 Thermus thermophilus
2.1.1.34 0.00205
-
 DELTAD-arm
-
 Thermus thermophilus