Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kumagai, I.; Watanabe, K.; Oshima, T.
    A thermostable tRNA (guanosine-2)-methyltransferase from Thermus thermophilus HB27 and the effect of ribose methylation on the conformational stability of tRNA (1982), J. Biol. Chem., 257, 7388-7395.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.34 spermine activation, 5 mM Thermus thermophilus

General Stability

EC Number General Stability Organism
2.1.1.34 urea, stable to 6 M Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.34 S-adenosyl-L-homocysteine strong, product inhibition, competitive Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.34 0.00001
-
 guanosine18 in Escherichia coli tRNA-Phe
-
 Thermus thermophilus
2.1.1.34 0.00047
-
 S-adenosyl-L-methionine
-
 Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.34 Mg2+ activation, 5 mM Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.34 20000
-
 gel filtration Thermus thermophilus
2.1.1.34 21000
-
 1 * 21000, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNATyr Thermus thermophilus involved in post-transcriptional tRNA modification S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNATyr Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 involved in post-transcriptional tRNA modification S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.34 Thermus thermophilus
-
-
-
2.1.1.34 Thermus thermophilus
-
 high growth temperature enhances activity
-
2.1.1.34 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.34 affinity chromatography on S-adenosyl-L-homocysteine-Sepharose, elution with 6 M urea Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.34 0.0071
-
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.34 additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus ?
-
 ?
2.1.1.34 additional information specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in Escherichia coli tRNA-Phe
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Phe
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptor from yeast: tRNAPhe Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNATyr involved in post-transcriptional tRNA modification Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNATyr involved in post-transcriptional tRNA modification Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.34 monomer 1 * 21000, SDS-PAGE Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.34 65
-
 assay at Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.34 80
-
 about 90% of original activity retained after 20 min in the presence of 10% v/v glycerol at pH 7.5 Thermus thermophilus
2.1.1.34 90
-
 t1/2: 20 min Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.34 7.2 7.5
-
 Thermus thermophilus