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Literature summary extracted from

  • Jhee, K.H.; Yoshimura, T.; Esaki, N.; Yonaha, K.; Soda, K.
    Thermostable ornithine aminotransferase from Bacillus sp. YM-2: purification and characterization (1995), J. Biochem., 118, 101-108.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.13 2
-
 L-ornithine 37°C Bacillus sp. (in: Bacteria)
2.6.1.13 6.1
-
 2-oxoglutarate 37°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.13 41000
-
 2 * 41000, SDS-PAGE Bacillus sp. (in: Bacteria)
2.6.1.13 82000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.13 Bacillus sp. (in: Bacteria)
-
 YM-2
-
2.6.1.13 Bacillus sp. (in: Bacteria) YM-2
-
 YM-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.13 homogeneity Bacillus sp. (in: Bacteria)

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.13 L-ornithine + a 2-oxo carboxylate = L-glutamate 5-semialdehyde + an L-amino acid mechanism Bacillus sp. (in: Bacteria)
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.13 76
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.13 D-ornithine + 2-oxoglutarate 6% the rate of L-ornithine Bacillus sp. (in: Bacteria) glutamate + DELTA1-pyrroline-5-carboxylate
-
 ?
2.6.1.13 D-ornithine + 2-oxoglutarate 6% the rate of L-ornithine Bacillus sp. (in: Bacteria) YM-2 glutamate + DELTA1-pyrroline-5-carboxylate
-
 ?
2.6.1.13 L-ornithine + 2-oxoglutarate
-
 Bacillus sp. (in: Bacteria) DELTA1-pyrroline-5-carboxylate + L-glutamate glutamate-gamma semialdehyde spontaneously cyclizes to form DELTA1-pyrroline-5-carboxylate ?
2.6.1.13 L-ornithine + 2-oxoglutarate
-
 Bacillus sp. (in: Bacteria) YM-2 DELTA1-pyrroline-5-carboxylate + L-glutamate glutamate-gamma semialdehyde spontaneously cyclizes to form DELTA1-pyrroline-5-carboxylate ?
2.6.1.13 L-ornithine + glyoxylate weak Bacillus sp. (in: Bacteria) DELTA1-pyrroline-5-carboxylate + glycine
-
 ?
2.6.1.13 L-ornithine + glyoxylate weak Bacillus sp. (in: Bacteria) YM-2 DELTA1-pyrroline-5-carboxylate + glycine
-
 ?
2.6.1.13 L-ornithine + pyruvate
-
 Bacillus sp. (in: Bacteria) DELTA1-pyrroline-5-carboxylate + L-alanine
-
 ?
2.6.1.13 L-ornithine + pyruvate
-
 Bacillus sp. (in: Bacteria) YM-2 DELTA1-pyrroline-5-carboxylate + L-alanine
-
 ?
2.6.1.13 ornithine + oxaloacetate weak Bacillus sp. (in: Bacteria) DELTA1-pyrroline-5-carboxylate + L-aspartate
-
 ?
2.6.1.13 ornithine + oxaloacetate weak Bacillus sp. (in: Bacteria) YM-2 DELTA1-pyrroline-5-carboxylate + L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.13 dimer 2 * 41000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.13 70
-
-
 Bacillus sp. (in: Bacteria)

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.13 pyridoxal 5'-phosphate 1 mol per mol of enzyme Bacillus sp. (in: Bacteria)