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Literature summary extracted from

  • Ohura, T.; Kominami, E.; Tada, K.; Katunuma, N.
    Crystallization and properties of human liver ornithine aminotransferase (1982), J. Biochem., 92, 1785-1792.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.13
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.13 1.1
-
 2-oxoglutarate pH 8, 37°C Rattus norvegicus
2.6.1.13 1.1
-
 L-ornithine pH 8, 37°C Rattus norvegicus
2.6.1.13 1.8
-
 L-ornithine pH 8, 37°C Homo sapiens
2.6.1.13 2.7
-
 2-oxoglutarate pH 8, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.13 44000
-
 4 * 44000, SDS-PAGE Homo sapiens
2.6.1.13 177000
-
 sucrose density gradient centrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.13 Homo sapiens
-
-
-
2.6.1.13 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.13
-
 Rattus norvegicus
2.6.1.13 partial Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.13 liver
-
 Homo sapiens
-
2.6.1.13 liver
-
 Rattus norvegicus
-
2.6.1.13 lymphoblast
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.13 16
-
-
 Rattus norvegicus
2.6.1.13 33
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.6.1.13 -20°C, 50 mM potassium phosphate buffer, pH 8, 5 mM 2-oxoglutarate, 0.02 mM pyridoxal phosphate, 90-100% retention of activity after 1 month Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.13 L-ornithine + 2-oxoglutarate
-
 Homo sapiens DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?
2.6.1.13 L-ornithine + 2-oxoglutarate
-
 Rattus norvegicus DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.13 tetramer
-
 Rattus norvegicus
2.6.1.13 tetramer 4 * 44000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.13 37
-
 assay at Homo sapiens
2.6.1.13 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.13 8
-
-
 Homo sapiens
2.6.1.13 8
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.13 7 9 pH 7.0: about 50% of activity maximum, pH 9.0: about 60% of activity maximum Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.13 pyridoxal 5'-phosphate
-
 Rattus norvegicus
2.6.1.13 pyridoxal 5'-phosphate Km: 0.0018 mM Homo sapiens