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Literature summary extracted from

  • Strecker, H.J.
    Purification and properties of rat liver ornithine DELTA-transaminase (1965), J. Biol. Chem., 240, 1225-1230.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.13 activity of the purified enzyme is preserved by addition of pyridoxal 5'-phosphate Rattus norvegicus
2.6.1.13 pyridoxal 5'-phosphate stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.13 2-oxobutanoate
-
 Rattus norvegicus
2.6.1.13 2-oxoglutarate alpha-keto acid acceptor, inhibitor at high concentrations Rattus norvegicus
2.6.1.13 2-Oxohexanoate
-
 Rattus norvegicus
2.6.1.13 2-oxoisopentanoate
-
 Rattus norvegicus
2.6.1.13 4-aminobutanoate
-
 Rattus norvegicus
2.6.1.13 5-Aminopentanoate
-
 Rattus norvegicus
2.6.1.13 cadaverine
-
 Rattus norvegicus
2.6.1.13 DL-norvaline
-
 Rattus norvegicus
2.6.1.13 glyoxylate alpha-keto acid acceptor, inhibitor at high concentrations Rattus norvegicus
2.6.1.13 L-canavanine complete inhibition at 0.025 M Rattus norvegicus
2.6.1.13 L-leucine
-
 Rattus norvegicus
2.6.1.13 L-valine
-
 Rattus norvegicus
2.6.1.13 ornithine at high concentrations Rattus norvegicus
2.6.1.13 putrescine
-
 Rattus norvegicus
2.6.1.13 pyruvate alpha-keto acid acceptor, inhibitor at high concentrations Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.13 0.28
-
 2-oxoglutarate pH 7.1, 37°C Rattus norvegicus
2.6.1.13 1.7
-
 ornithine with pyruvate as cosubstrate, pH 7.1, 37°C Rattus norvegicus
2.6.1.13 2.8
-
 ornithine with 2-oxoglutarate as cosubstrate, pH 7.1, 37°C Rattus norvegicus
2.6.1.13 2.9
-
 ornithine with glyoxylate as cosubstrate, pH 7.1, 37°C Rattus norvegicus
2.6.1.13 6.7
-
 glyoxylate pH 7.1, 37°C Rattus norvegicus
2.6.1.13 22
-
 pyruvate pH 7.1, 37°C Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.13 L-ornithine + 2-oxoglutarate Rattus norvegicus metabolism of proline to ornithine DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.13 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.13 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.13 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.13 0.21
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.13 -15°C, rat liver homogenate in sucrose or phosphate solution, stable for some weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.13 L-ornithine + 2-oxoglutarate D-ornithine is not accepted as substrate Rattus norvegicus DELTA1-pyrroline-5-carboxylate + L-glutamate equilibrium constant: 71 ?
2.6.1.13 L-ornithine + 2-oxoglutarate metabolism of proline to ornithine Rattus norvegicus DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?
2.6.1.13 L-ornithine + glyoxylate
-
 Rattus norvegicus DELTA1-pyrroline-5-carboxylate + glycine
-
 ?
2.6.1.13 L-ornithine + pyruvate
-
 Rattus norvegicus DELTA1-pyrroline-5-carboxylate + L-alanine
-
 ?
2.6.1.13 additional information inactive as amino group acceptor: 2-oxoisohexanoate Rattus norvegicus ?
-
 ?
2.6.1.13 additional information inactive as amino group acceptor: 2-oxoisopentanoate Rattus norvegicus ?
-
 ?
2.6.1.13 additional information inactive as amino group acceptor: oxaloacetate Rattus norvegicus ?
-
 ?
2.6.1.13 additional information specific for ornithine Rattus norvegicus ?
-
 ?
2.6.1.13 additional information inactive as amino group acceptor: 2-oxobutanoate Rattus norvegicus ?
-
 ?
2.6.1.13 additional information inactive as amino group acceptor: 2-oxopentanoate Rattus norvegicus ?
-
 ?
2.6.1.13 additional information inactive as amino group acceptor: 2-oxohexanoate Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.13 37
-
 assay at Rattus norvegicus