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Literature summary extracted from

  • Maître, M.; Ciesielski, L.; Cash, C.; Mandel, P.
    Purification and studies on some properties of the 4-aminobutyrate: 2-oxoglutarate transaminase from rat brain (1975), Eur. J. Biochem., 52, 157-169.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.19 2-oxoglutarate
-
 Rattus norvegicus
2.6.1.19 Succinic semialdehyde substrate inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.19 additional information
-
 additional information kinetic study Rattus norvegicus
2.6.1.19 additional information
-
 additional information pH-dependence of kinetic data Rattus norvegicus
2.6.1.19 4
-
 4-aminobutanoate 38°C, pH 8.5 Rattus norvegicus
2.6.1.19 5.5
-
 2-oxoglutarate 38°C, pH 8.5 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.19 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.19 57000
-
 2 * 57000, SDS-PAGE with or without DTT or urea Rattus norvegicus
2.6.1.19 103000
-
 PAGE Rattus norvegicus
2.6.1.19 105000
-
 gel filtration Rattus norvegicus
2.6.1.19 107000
-
 calculated from amino acid composition Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.19 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.19
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.19 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.19 10
-
 38°C, pH 8.5 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.19 4-aminobutanoate + 2-oxoglutarate
-
 Rattus norvegicus 4-oxobutanoate + L-glutamate
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.19 dimer 2 * 57000, SDS-PAGE with or without DTT or urea Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.19 45
-
-
 Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.19 30 55 about half-maximal activity at 30°C and 55°C Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.19 35
-
 t1/2: 3 h Rattus norvegicus
2.6.1.19 50
-
 3 h, 80% loss of activity Rattus norvegicus
2.6.1.19 55
-
 3 h, inactivation Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.19 additional information
-
 pI: 6.8 Rattus norvegicus
2.6.1.19 8.5
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.19 7.4 9 about half-maximal activity at pH 7.4 and about 90% of maximal activity at pH 9 Rattus norvegicus
2.6.1.19 8 9.2 about 50% of maximal activity at pH 9.2 Rattus norvegicus
2.6.1.19 8 9.2 about half-maximal activity at pH 8 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.19 0.00018
-
 2-oxoglutarate 38°C, pH 8.5 Rattus norvegicus
2.6.1.19 0.0066
-
 Succinic semialdehyde 38°C, pH 8.5 Rattus norvegicus