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Literature summary extracted from

  • Chapple, C.C.S.; Glover, J.R.; Ellis, B.E.
    Purification and characterization of methionine:glyoxylate aminotransferase from Brassica carinata and Brassica napus (1990), Plant Physiol., 94, 1887-1896.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.73 49000
-
 x * 49000, SDS-PAGE Brassica carinata
2.6.1.73 51000
-
 x * 51000, SDS-PAGE Brassica napus
2.6.1.73 230000 290000 gel filtration Brassica napus
2.6.1.73 230000 290000 gel filtration Brassica carinata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.73 additional information Brassica napus function in the deamination of methionine or production of methionine in the methionine-cycle ?
-
 ?
2.6.1.73 additional information Brassica carinata function in the deamination of methionine or production of methionine in the methionine-cycle ?
-
 ?
2.6.1.73 additional information Brassica carinata involved in the biosynthesis of methionine-derived glucosinolates ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.73 Brassica carinata
-
 var. R-4218
-
2.6.1.73 Brassica napus
-
 var. Topas
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.73 purification on Mono Q results in isolation of three isoenzymes, MGAT 1, MGAT 2 and MGAT 3 Brassica carinata
2.6.1.73 var. R-4218, partial Brassica carinata
2.6.1.73 var. Topas, partial Brassica napus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.73 leaf
-
 Brassica napus
-
2.6.1.73 leaf
-
 Brassica carinata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.73 0.06
-
 isoenzyme MGAT 3, 30°C, pH 8.2 Brassica carinata
2.6.1.73 0.066
-
 MGAT, single enzyme 30°C, pH 8.2 Brassica napus
2.6.1.73 0.18
-
 isoenzyme MGAT 2 30°C, pH 8.2 Brassica carinata
2.6.1.73 0.6
-
 isoenzyme MGAT 1, overall activity without separation of isoenzymes 30°C, pH 8.2 Brassica carinata

Storage Stability

EC Number Storage Stability Organism
2.6.1.73 -60°C, desalted enzyme preparation Brassica napus
2.6.1.73 -60°C, desalted enzyme preparation Brassica carinata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.73 L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
 Brassica napus 2-oxoglutarate + L-methionine
-
 ?
2.6.1.73 L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
 Brassica carinata 2-oxoglutarate + L-methionine
-
 ?
2.6.1.73 L-glutamine + glyoxylate
-
 Brassica napus 2-oxoglutarate + glycine
-
 ?
2.6.1.73 L-glutamine + glyoxylate
-
 Brassica carinata 2-oxoglutarate + glycine
-
 ?
2.6.1.73 L-methionine + 4-methylsulfanyl-2-oxobutanoate
-
 Brassica napus 4-methylsulfanyl-2-oxobutanoate + L-methionine
-
 ?
2.6.1.73 L-methionine + 4-methylsulfanyl-2-oxobutanoate
-
 Brassica carinata 4-methylsulfanyl-2-oxobutanoate + L-methionine
-
 ?
2.6.1.73 L-methionine + glyoxylate
-
 Brassica napus 4-methylsulfanyl-2-oxobutanoate + glycine
-
 ?
2.6.1.73 L-methionine + glyoxylate
-
 Brassica carinata 4-methylsulfanyl-2-oxobutanoate + glycine
-
 ?
2.6.1.73 L-methionine + oxaloacetate 24% of activity compared to glyoxylate Brassica carinata 4-methylsulfanyl-2-oxobutanoate + L-aspartate
-
 ?
2.6.1.73 additional information function in the deamination of methionine or production of methionine in the methionine-cycle Brassica napus ?
-
 ?
2.6.1.73 additional information function in the deamination of methionine or production of methionine in the methionine-cycle Brassica carinata ?
-
 ?
2.6.1.73 additional information involved in the biosynthesis of methionine-derived glucosinolates Brassica carinata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.73 ? x * 51000, SDS-PAGE Brassica napus
2.6.1.73 ? x * 49000, SDS-PAGE Brassica carinata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.73 30
-
 assay at Brassica napus
2.6.1.73 30
-
 assay at Brassica carinata