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Literature summary extracted from

  • Kamel, M.Y.; Anderson, R.L.
    Acyl phosphate: hexose phosphotransferase. Purification and properties of the enzyme from Aerobacter aerogenes and evidence for its common identity with hexose phosphate: hexose phosphotransferase (1967), Arch. Biochem. Biophys., 120, 322-331.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.61 stable to repeated freezing and thawing Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.61 additional information not inhibitory: EDTA Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.61 0.16
-
D-glucose pH 7.5, 25°C Klebsiella aerogenes
2.7.1.61 0.4
-
acetyl phosphate carbamoyl phosphate, D-mannose 6-phosphate, pH 7.5, 25°C Klebsiella aerogenes
2.7.1.61 2
-
D-ribose 5-phosphate pH 7.5, 25°C Klebsiella aerogenes
2.7.1.61 12
-
D-mannose pH 7.5, 25°C Klebsiella aerogenes
2.7.1.61 67
-
mannitol pH 7.5, 25°C Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.61 additional information no stimulation by addition of various cations Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.61 acyl phosphate + D-hexose Klebsiella aerogenes
-
an acid + D-hexose phosphate
-
?
2.7.1.61 D-glucose + acetyl phosphate Klebsiella aerogenes
-
D-glucose 6-phosphate + acetate
-
?
2.7.1.61 D-glucose + acetyl phosphate Klebsiella aerogenes PRL-R3
-
D-glucose 6-phosphate + acetate
-
?
2.7.1.61 D-glucose + D-mannose 6-phosphate Klebsiella aerogenes
-
D-glucose 6-phosphate + D-mannose
-
?
2.7.1.61 D-glucose + D-mannose 6-phosphate Klebsiella aerogenes PRL-R3
-
D-glucose 6-phosphate + D-mannose
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.61 Klebsiella aerogenes
-
PRL-R3
-
2.7.1.61 Klebsiella aerogenes PRL-R3
-
PRL-R3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.61
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.61 1.3
-
pH 7.5, 25°C Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.61 acyl phosphate + D-hexose
-
Klebsiella aerogenes an acid + D-hexose phosphate
-
?
2.7.1.61 D-fructose + acetyl phosphate
-
Klebsiella aerogenes D-fructose 1-phosphate + D-fructose 6-phosphate + acetate
-
?
2.7.1.61 D-glucose + acetyl phosphate
-
Klebsiella aerogenes D-glucose 6-phosphate + acetate
-
?
2.7.1.61 D-glucose + acetyl phosphate
-
Klebsiella aerogenes PRL-R3 D-glucose 6-phosphate + acetate
-
?
2.7.1.61 D-glucose + carbamoyl phosphate
-
Klebsiella aerogenes D-glucose 6-phosphate + carbamate
-
?
2.7.1.61 D-glucose + carbamoyl phosphate
-
Klebsiella aerogenes PRL-R3 D-glucose 6-phosphate + carbamate
-
?
2.7.1.61 D-glucose + D-fructose 1-phosphate 15% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes D-glucose 6-phosphate + D-fructose
-
?
2.7.1.61 D-glucose + D-fructose 6-phosphate 25% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes D-glucose 6-phosphate + D-fructose
-
?
2.7.1.61 D-glucose + D-glucose 1-phosphate 5% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes D-glucose 6-phosphate + D-glucose
-
?
2.7.1.61 D-glucose + D-mannose 6-phosphate
-
Klebsiella aerogenes D-glucose 6-phosphate + D-mannose
-
?
2.7.1.61 D-glucose + D-mannose 6-phosphate 71% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes D-glucose 6-phosphate + D-mannose
-
?
2.7.1.61 D-glucose + D-mannose 6-phosphate
-
Klebsiella aerogenes PRL-R3 D-glucose 6-phosphate + D-mannose
-
?
2.7.1.61 D-glucose + D-mannose 6-phosphate 71% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes PRL-R3 D-glucose 6-phosphate + D-mannose
-
?
2.7.1.61 D-glucose + D-ribose 5-phosphate 18% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes D-glucose 6-phosphate + D-ribose
-
?
2.7.1.61 D-glucose + D-sorbitol 6-phosphate 2.5% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes D-glucose 6-phosphate + D-sorbitol
-
?
2.7.1.61 D-mannitol + acetyl phosphate
-
Klebsiella aerogenes ?
-
?
2.7.1.61 D-mannose + acetyl phosphate
-
Klebsiella aerogenes D-mannose 6-phosphate + acetate
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.61 9
-
-
Klebsiella aerogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.61 7.5 9 pH 7.5: 50% of maximum activity, pH 9: maximum activity Klebsiella aerogenes