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Literature summary extracted from

  • Anderson, R.L.; Kamel, M.Y.
    Acyl phosphate:hexose phosphotransferase (hexose phosphate:hexose phosphotransferase) (1966), Methods Enzymol., 9, 392-396.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.61 stable to repeated freezing and thawing Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.61 D-mannose inhibits phosphorylation of D-glucose, competitive to acetyl phosphate or D-mannose 6-phosphate Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.61 0.16
-
 D-glucose pH 7.5, 25°C Klebsiella aerogenes
2.7.1.61 0.4
-
 acetyl phosphate carbamoyl phosphate, D-mannose 6-phosphate, pH 7.5, 25°C Klebsiella aerogenes
2.7.1.61 2
-
 D-ribose 5-phosphate pH 7.5, 25°C Klebsiella aerogenes
2.7.1.61 12
-
 D-mannose pH 7.5, 25°C Klebsiella aerogenes
2.7.1.61 67
-
 mannitol pH 7.5, 25°C Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.61 D-glucose + acetyl phosphate Klebsiella aerogenes
-
 D-glucose 6-phosphate + acetate
-
 ?
2.7.1.61 D-glucose + acetyl phosphate Klebsiella aerogenes PRL-R3
-
 D-glucose 6-phosphate + acetate
-
 ?
2.7.1.61 D-glucose + D-mannose 6-phosphate Klebsiella aerogenes
-
 D-glucose 6-phosphate + D-mannose
-
 ?
2.7.1.61 D-glucose + D-mannose 6-phosphate Klebsiella aerogenes PRL-R3
-
 D-glucose 6-phosphate + D-mannose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.61 Klebsiella aerogenes
-
 PRL-R3
-
2.7.1.61 Klebsiella aerogenes PRL-R3
-
 PRL-R3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.61
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.61 1.3
-
 pH 7.5, 25°C Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.7.1.61 -20°C, stable Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.61 D-fructose + acetyl phosphate
-
 Klebsiella aerogenes D-fructose 1-phosphate + D-fructose 6-phosphate + acetate D-fructose 1-phosphate is the predominant product ?
2.7.1.61 D-glucose + acetyl phosphate
-
 Klebsiella aerogenes D-glucose 6-phosphate + acetate
-
 ?
2.7.1.61 D-glucose + acetyl phosphate
-
 Klebsiella aerogenes PRL-R3 D-glucose 6-phosphate + acetate
-
 ?
2.7.1.61 D-glucose + carbamoyl phosphate
-
 Klebsiella aerogenes D-glucose 6-phosphate + carbamate
-
 ?
2.7.1.61 D-glucose + carbamoyl phosphate
-
 Klebsiella aerogenes PRL-R3 D-glucose 6-phosphate + carbamate
-
 ?
2.7.1.61 D-glucose + D-fructose 1-phosphate 15% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes D-glucose 6-phosphate + D-fructose
-
 ?
2.7.1.61 D-glucose + D-fructose 6-phosphate 25% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes D-glucose 6-phosphate + D-fructose
-
 ?
2.7.1.61 D-glucose + D-glucose 1-phosphate 5% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes D-glucose 6-phosphate + D-glucose
-
 ?
2.7.1.61 D-glucose + D-mannose 6-phosphate
-
 Klebsiella aerogenes D-glucose 6-phosphate + D-mannose
-
 ?
2.7.1.61 D-glucose + D-mannose 6-phosphate 71% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes D-glucose 6-phosphate + D-mannose
-
 r
2.7.1.61 D-glucose + D-mannose 6-phosphate
-
 Klebsiella aerogenes PRL-R3 D-glucose 6-phosphate + D-mannose
-
 ?
2.7.1.61 D-glucose + D-mannose 6-phosphate 71% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes PRL-R3 D-glucose 6-phosphate + D-mannose
-
 r
2.7.1.61 D-glucose + D-ribose 5-phosphate 18% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes D-glucose 6-phosphate + D-ribose
-
 ?
2.7.1.61 D-glucose + D-sorbitol 6-phosphate 2.5% of the activity with acetyl phosphate or carbamoyl phosphate Klebsiella aerogenes D-glucose 6-phosphate + D-sorbitol
-
 ?
2.7.1.61 D-mannitol + acetyl phosphate
-
 Klebsiella aerogenes ?
-
 ?
2.7.1.61 D-mannose + acetyl phosphate
-
 Klebsiella aerogenes D-mannose 6-phosphate + acetate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.61 25
-
 assay at Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.61 9
-
-
 Klebsiella aerogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.61 7.5 9 pH 7.5: 50% of maximum activity, pH 9: maximum activity Klebsiella aerogenes