EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.22 | 5-fluorouracil | - |
Rattus norvegicus | |
2.6.1.22 | 5-Iodouracil | - |
Rattus norvegicus | |
2.6.1.22 | 6-Azathymine | - |
Rattus norvegicus | |
2.6.1.22 | 6-Azauracil | - |
Rattus norvegicus | |
2.6.1.22 | gabaculine | - |
Rattus norvegicus | |
2.6.1.22 | additional information | not inhibitory: 6-azauridine, 6-azauridine 5-phosphate | Rattus norvegicus | |
2.6.1.40 | alpha-fluoro-beta-alanine | - |
Rattus norvegicus | |
2.6.1.40 | gabaculine | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.22 | 1.1 | - |
beta-Alanine | pH 8.8, 37°C | Rattus norvegicus | |
2.6.1.22 | 2.7 | - |
(S)-3-amino-2-methylpropanoate | pH 8.8, 37°C | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.6.1.22 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.22 | 105000 | - |
gel filtration | Rattus norvegicus |
2.6.1.40 | 52000 | - |
4 * 52000, SDS-PAGE | Rattus norvegicus |
2.6.1.40 | 220000 | - |
gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.22 | Rattus norvegicus | - |
- |
- |
2.6.1.40 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.22 | - |
Rattus norvegicus |
2.6.1.40 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.6.1.22 | brain | - |
Rattus norvegicus | - |
2.6.1.22 | liver | - |
Rattus norvegicus | - |
2.6.1.40 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.22 | 0.047 | - |
kidney enzyme, pH 8.8, 37°C | Rattus norvegicus |
2.6.1.22 | 0.096 | - |
brain enzyme, pH 8.8, 37°C | Rattus norvegicus |
2.6.1.22 | 0.133 | - |
liver enzyme, pH 8.8, 37°C | Rattus norvegicus |
2.6.1.40 | additional information | - |
assay principle | Rattus norvegicus |
2.6.1.40 | 11.3 | - |
pH 8.8, 37°C | Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.6.1.22 | -25°C, in 0.1 M phosphate buffer, pH 7.0, 1mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal-5'-phosphate, few days, 50% loss of activity | Rattus norvegicus |
2.6.1.22 | 4°C, in 0.1 M phosphate buffer, pH 7.0, 1mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal-5'-phosphate, 1 week, no loss of activity | Rattus norvegicus |
2.6.1.40 | 4°C, 0.05 mg protein/ml, in 0.1 M potassium phosphate buffer, pH 7, 1 mM EDTA, 2 mM 2-mercaptoethanol, about 50% loss of activity within a few days, 80% loss within 1 week | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.22 | (S)-3-amino-2-methylpropanoate + pyruvate | - |
Rattus norvegicus | D-methylmalonate semialdehyde + L-alanine | - |
r | |
2.6.1.22 | 4-aminobutanoate + 2-oxoglutarate | transamination at 150% the rate of (S)-3-amino-2-methylpropanoate | Rattus norvegicus | succinic semialdehyde + L-glutamate | - |
r | |
2.6.1.22 | 5-aminopentanoate + 2-oxoglutarate | transamination at 145% the rate of (S)-3-amino-2-methylpropanoate | Rattus norvegicus | 5-oxopentanoate + L-glutamate | - |
r | |
2.6.1.22 | beta-alanine + 2-oxoglutarate | as good as (S)-3-amino-2-methylpropanoate | Rattus norvegicus | malonic semialdehyde + L-glutamate | - |
r | |
2.6.1.22 | additional information | no substrates are glyoxylate, pyruvate, oxalacetate, phenylpyruvate | Rattus norvegicus | ? | - |
? | |
2.6.1.40 | (R)-3-amino-2-methylpropanoate + glyoxylate | with 90% efficiency compared to pyruvate | Rattus norvegicus | 2-methyl-3-oxopropanoate + glycine | - |
r | |
2.6.1.40 | (R)-3-amino-2-methylpropanoate + oxaloacetate | with 63% efficiency compared to pyruvate | Rattus norvegicus | 2-methyl-3-oxopropanoate + L-aspartate | - |
r | |
2.6.1.40 | beta-alanine + pyruvate | transamination at 66% the rate of (R)-3-amino-2-methylpropanoate | Rattus norvegicus | malonic semialdehyde + L-alanine | - |
? | |
2.6.1.40 | additional information | overview | Rattus norvegicus | ? | - |
? | |
2.6.1.40 | additional information | no substrate: 2-oxoglutarate | Rattus norvegicus | ? | - |
? | |
2.6.1.40 | additional information | gamma-aminobutanoate is neither substrate nor inhibitor | Rattus norvegicus | ? | - |
? | |
2.6.1.40 | additional information | no substrate: phenylpyruvate | Rattus norvegicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.22 | dimer | 2 x 56000, SDS-PAGE | Rattus norvegicus |
2.6.1.40 | tetramer | 4 * 52000, SDS-PAGE | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.22 | 9.2 | - |
- |
Rattus norvegicus |
2.6.1.40 | 9.5 | - |
- |
Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.22 | 0.0071 | - |
gabaculine | pH 8.8, 37°C | Rattus norvegicus | |
2.6.1.22 | 0.7 | - |
6-Azauracil | pH 8.8, 37°C | Rattus norvegicus | |
2.6.1.22 | 1.8 | - |
5-fluorouracil | uncompetitive against 2-oxoglutarate, pH 8.8, 37°C | Rattus norvegicus | |
2.6.1.22 | 1.9 | - |
5-fluorouracil | competitive to beta-alanine, pH 8.8, 37°C | Rattus norvegicus | |
2.6.1.40 | additional information | - |
additional information | gamma-aminobutanoate is neither substrate nor inhibitor | Rattus norvegicus |