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Literature summary extracted from

  • Tamaki, N.; Sakata, S.F.; Matsuda, K.
    Purification, properties, and sequencing of aminoisobutyrate aminotransferases from rat liver (2000), Methods Enzymol., 324, 376-389.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.22 5-fluorouracil
-
Rattus norvegicus
2.6.1.22 5-Iodouracil
-
Rattus norvegicus
2.6.1.22 6-Azathymine
-
Rattus norvegicus
2.6.1.22 6-Azauracil
-
Rattus norvegicus
2.6.1.22 gabaculine
-
Rattus norvegicus
2.6.1.22 additional information not inhibitory: 6-azauridine, 6-azauridine 5’-phosphate Rattus norvegicus
2.6.1.40 alpha-fluoro-beta-alanine
-
Rattus norvegicus
2.6.1.40 gabaculine
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.22 1.1
-
beta-Alanine pH 8.8, 37°C Rattus norvegicus
2.6.1.22 2.7
-
(S)-3-amino-2-methylpropanoate pH 8.8, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.22 mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.22 105000
-
gel filtration Rattus norvegicus
2.6.1.40 52000
-
4 * 52000, SDS-PAGE Rattus norvegicus
2.6.1.40 220000
-
gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.22 Rattus norvegicus
-
-
-
2.6.1.40 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.22
-
Rattus norvegicus
2.6.1.40
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.22 brain
-
Rattus norvegicus
-
2.6.1.22 liver
-
Rattus norvegicus
-
2.6.1.40 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.22 0.047
-
kidney enzyme, pH 8.8, 37°C Rattus norvegicus
2.6.1.22 0.096
-
brain enzyme, pH 8.8, 37°C Rattus norvegicus
2.6.1.22 0.133
-
liver enzyme, pH 8.8, 37°C Rattus norvegicus
2.6.1.40 additional information
-
assay principle Rattus norvegicus
2.6.1.40 11.3
-
pH 8.8, 37°C Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.22 -25°C, in 0.1 M phosphate buffer, pH 7.0, 1mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal-5'-phosphate, few days, 50% loss of activity Rattus norvegicus
2.6.1.22 4°C, in 0.1 M phosphate buffer, pH 7.0, 1mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal-5'-phosphate, 1 week, no loss of activity Rattus norvegicus
2.6.1.40 4°C, 0.05 mg protein/ml, in 0.1 M potassium phosphate buffer, pH 7, 1 mM EDTA, 2 mM 2-mercaptoethanol, about 50% loss of activity within a few days, 80% loss within 1 week Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.22 (S)-3-amino-2-methylpropanoate + pyruvate
-
Rattus norvegicus D-methylmalonate semialdehyde + L-alanine
-
r
2.6.1.22 4-aminobutanoate + 2-oxoglutarate transamination at 150% the rate of (S)-3-amino-2-methylpropanoate Rattus norvegicus succinic semialdehyde + L-glutamate
-
r
2.6.1.22 5-aminopentanoate + 2-oxoglutarate transamination at 145% the rate of (S)-3-amino-2-methylpropanoate Rattus norvegicus 5-oxopentanoate + L-glutamate
-
r
2.6.1.22 beta-alanine + 2-oxoglutarate as good as (S)-3-amino-2-methylpropanoate Rattus norvegicus malonic semialdehyde + L-glutamate
-
r
2.6.1.22 additional information no substrates are glyoxylate, pyruvate, oxalacetate, phenylpyruvate Rattus norvegicus ?
-
?
2.6.1.40 (R)-3-amino-2-methylpropanoate + glyoxylate with 90% efficiency compared to pyruvate Rattus norvegicus 2-methyl-3-oxopropanoate + glycine
-
r
2.6.1.40 (R)-3-amino-2-methylpropanoate + oxaloacetate with 63% efficiency compared to pyruvate Rattus norvegicus 2-methyl-3-oxopropanoate + L-aspartate
-
r
2.6.1.40 beta-alanine + pyruvate transamination at 66% the rate of (R)-3-amino-2-methylpropanoate Rattus norvegicus malonic semialdehyde + L-alanine
-
?
2.6.1.40 additional information overview Rattus norvegicus ?
-
?
2.6.1.40 additional information no substrate: 2-oxoglutarate Rattus norvegicus ?
-
?
2.6.1.40 additional information gamma-aminobutanoate is neither substrate nor inhibitor Rattus norvegicus ?
-
?
2.6.1.40 additional information no substrate: phenylpyruvate Rattus norvegicus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.22 dimer 2 x 56000, SDS-PAGE Rattus norvegicus
2.6.1.40 tetramer 4 * 52000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.22 9.2
-
-
Rattus norvegicus
2.6.1.40 9.5
-
-
Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.22 0.0071
-
gabaculine pH 8.8, 37°C Rattus norvegicus
2.6.1.22 0.7
-
6-Azauracil pH 8.8, 37°C Rattus norvegicus
2.6.1.22 1.8
-
5-fluorouracil uncompetitive against 2-oxoglutarate, pH 8.8, 37°C Rattus norvegicus
2.6.1.22 1.9
-
5-fluorouracil competitive to beta-alanine, pH 8.8, 37°C Rattus norvegicus
2.6.1.40 additional information
-
additional information gamma-aminobutanoate is neither substrate nor inhibitor Rattus norvegicus