BRENDA - Enzyme Database show

beta-Aminoisobutyrate-alpha-ketoglutarate transaminase in relation to beta-aminoisobutyric aciduria

Kakimoto, Y.; Kanazawa, A.; Taniguchi, K.; Sano, I.; Biochim. Biophys. Acta 156, 374-380 (1968)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.22
2-oxoglutarate
in excess, substrate inhibition
Sus scrofa
2.6.1.22
pyridoxal 5'-phosphate
inhibits at high concentrations, activates at low concentrations
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.22
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.22
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.22
kidney
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.6.1.22
0.185
-
pH 7.5, 37C
Sus scrofa
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.22
-20C, in 0.05 M phosphate buffer, pH 7.5, at least 3 weeks
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.22
(S)-3-amino-2-methylpropanoate + 2-oxoglutarate
i.e. L-beta-aminoisobutyrate, not D-isomer
636962
Sus scrofa
2-methyl-3-oxopropanoate + L-glutamate
-
636962
Sus scrofa
r
2.6.1.22
4-aminobutanoate + 2-oxoglutarate
transamination at 92% the rate of (S)-3-amino-2-methylpropanoate
636962
Sus scrofa
succinic semialdehyde + L-glutamate
-
636962
Sus scrofa
?
2.6.1.22
5-aminopentanoate + 2-oxoglutarate
transamination at 33% the rate of (S)-3-amino-2-methylpropanoate
636962
Sus scrofa
5-oxopentanoate + L-glutamate
-
-
-
?
2.6.1.22
beta-alanine + 2-oxoglutarate
as good as (S)-3-amino-2-methylpropanoate
636962
Sus scrofa
malonic semialdehyde + L-glutamate
-
-
-
?
2.6.1.22
additional information
no substrates are 3-aminobutanoate, 2-ethyl-beta-alanine, glycine, L-alpha-alanine, 2-aminoisobutanoate, lysine, taurine, O-phosphoethanolamine, homocarnosine. Poor substrates are 6-aminohexanoate or ornithine, no substrates are pyruvate, oxaloacetate or glyoxylate
636962
Sus scrofa
?
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.6.1.22
37
-
assay at
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.22
9.1
-
-
Sus scrofa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.6.1.22
8.5
9.5
about half-maximal activity at pH 8.5 and 9.5
Sus scrofa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.22
pyridoxal 5'-phosphate
activates at low concentrations, inhibits at high concentrations
Sus scrofa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.22
pyridoxal 5'-phosphate
activates at low concentrations, inhibits at high concentrations
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.22
2-oxoglutarate
in excess, substrate inhibition
Sus scrofa
2.6.1.22
pyridoxal 5'-phosphate
inhibits at high concentrations, activates at low concentrations
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.22
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.22
kidney
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.6.1.22
0.185
-
pH 7.5, 37C
Sus scrofa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.22
-20C, in 0.05 M phosphate buffer, pH 7.5, at least 3 weeks
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.22
(S)-3-amino-2-methylpropanoate + 2-oxoglutarate
i.e. L-beta-aminoisobutyrate, not D-isomer
636962
Sus scrofa
2-methyl-3-oxopropanoate + L-glutamate
-
636962
Sus scrofa
r
2.6.1.22
4-aminobutanoate + 2-oxoglutarate
transamination at 92% the rate of (S)-3-amino-2-methylpropanoate
636962
Sus scrofa
succinic semialdehyde + L-glutamate
-
636962
Sus scrofa
?
2.6.1.22
5-aminopentanoate + 2-oxoglutarate
transamination at 33% the rate of (S)-3-amino-2-methylpropanoate
636962
Sus scrofa
5-oxopentanoate + L-glutamate
-
-
-
?
2.6.1.22
beta-alanine + 2-oxoglutarate
as good as (S)-3-amino-2-methylpropanoate
636962
Sus scrofa
malonic semialdehyde + L-glutamate
-
-
-
?
2.6.1.22
additional information
no substrates are 3-aminobutanoate, 2-ethyl-beta-alanine, glycine, L-alpha-alanine, 2-aminoisobutanoate, lysine, taurine, O-phosphoethanolamine, homocarnosine. Poor substrates are 6-aminohexanoate or ornithine, no substrates are pyruvate, oxaloacetate or glyoxylate
636962
Sus scrofa
?
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.6.1.22
37
-
assay at
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.22
9.1
-
-
Sus scrofa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.6.1.22
8.5
9.5
about half-maximal activity at pH 8.5 and 9.5
Sus scrofa