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Literature summary extracted from

  • Cynkin, M.A.; Ashwell, G.
    Uronic acid metabolism in bacteria: IV. Purification and properties of 2-keto-3-deoxy-D-gluconokinase in Escherichia coli (1969), J. Biol. Chem., 235, 1576-1579.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.45 Mn2+ divalent cation required, most effective at a ratio of Mn2+ and ATP of 1:3, deviation from this ratio is inhibitory at several concentration levels Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.45 1
-
 2-dehydro-3-deoxy-D-gluconate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.45 Co2+ divalent cation required Escherichia coli
2.7.1.45 Mg2+ divalent cation required Escherichia coli
2.7.1.45 Mn2+ divalent cation required, most effective at a ratio of Mn2+ and ATP of 1:3, deviation from this ratio is inhibitory at several concentration levels Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.45 Escherichia coli
-
-
-
2.7.1.45 Klebsiella aerogenes
-
-
-
2.7.1.45 no activity in Azobacter agilis
-
-
-
2.7.1.45 no activity in Neurospora crassa
-
-
-
2.7.1.45 no activity in Salmonella typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.45
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.45 additional information
-
 assay method Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.1.45 -15°C, retains more than 50% of activity after 2 weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.45 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Escherichia coli ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.45 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Klebsiella aerogenes ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.45 CTP + 2-dehydro-3-deoxy-D-gluconate 8% of the activity with ATP Escherichia coli CDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.45 CTP + 2-dehydro-3-deoxy-D-gluconate 8% of the activity with ATP Klebsiella aerogenes CDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.45 GTP + 2-dehydro-3-deoxy-D-gluconate 34% of the activity with ATP Escherichia coli GDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.45 GTP + 2-dehydro-3-deoxy-D-gluconate 34% of the activity with ATP Klebsiella aerogenes GDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.45 ITP + 2-dehydro-3-deoxy-D-gluconate 75% of the activity with ATP Escherichia coli IDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.45 ITP + 2-dehydro-3-deoxy-D-gluconate 75% of the activity with ATP Klebsiella aerogenes IDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.45 UTP + 2-dehydro-3-deoxy-D-gluconate 7% of the activity with ATP Escherichia coli UDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.45 UTP + 2-dehydro-3-deoxy-D-gluconate 7% of the activity with ATP Klebsiella aerogenes UDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.45 5.6 6 acetate buffer Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.45 4.7 8 pH 4.7: about 50% of maximum activity, pH 8: about 85% of maximum activity, acetate buffer Escherichia coli