EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.18 | - |
Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.18 | Y300A | mutant enzyme shows less than 1% of the wild-type activity | Escherichia coli |
2.4.1.18 | Y300D | mutant enzyme shows less than 1% of the wild-type activity | Escherichia coli |
2.4.1.18 | Y300F | mutant enzyme shows 25% of the wild-type activity, no effect on Km-value, heat stability is lowered significantly compared to that of the wild-type enzyme, lower relative activity at elevated temperatures compared to wild-type enzyme | Escherichia coli |
2.4.1.18 | Y300L | mutant enzyme shows less than 1% of the wild-type activity | Escherichia coli |
2.4.1.18 | Y300S | mutant enzyme shows less than 1% of the wild-type activity | Escherichia coli |
2.4.1.18 | Y300W | mutant enzyme shows less than 1% of the wild-type activity | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.18 | 0.0142 | - |
amylose | amylose AS-320, pH 7.0, 30°C, wild-type enzyme | Escherichia coli | |
2.4.1.18 | 0.0175 | - |
amylose | amylose AS-320, pH 7.0, 30°C, mutant enzyme Y300F | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.18 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.18 | amylose | wild-type enzyme and mutant enzyme Y300F both preferentially transfer chains between DP5 and DP16, with a chain of DP11 being transferred at the highest frequency | Escherichia coli | amylose containing alpha-1,6-glucosidic linkages | - |
? |