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Literature summary extracted from

  • Hegde, S.S.; Dam, T.K.; Brewer, C.F.; Blanchard, J.S.
    Thermodynamics of aminoglycoside and acyl-Coenzyme A binding to the Salmonella enterica AAC(6')-Iy aminoglycoside N-acetyltransferase (2002), Biochemistry, 41, 7519-7527.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.82 C109A/C70A mutant enzyme is not inactivated by iodoacetamide. Double mutant exhibits large increases in Km-values for both acetyl-CoA and aminoglycoside substrates Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.82 additional information Salmonella enterica involved in resistance to antibiotics ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Salmonella enterica
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 wild-type enzyme and mutant enzymes C109A and C109A/C70A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.82
-
 Salmonella enterica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.82 additional information
-
 equilibrium binding and the directly determined thermodynamic parameters for different aminoglycosides and acyl-CoA derivatives to the wild-type enzyme and two mutant enzymes c109A and C109A/C70A using fluorescence spectroscopy and isothermal titration calorimetry Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 additional information C70 is directly involved in aminoglycoside binding Salmonella enterica ?
-
 ?
2.3.1.82 additional information involved in resistance to antibiotics Salmonella enterica ?
-
 ?