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Literature summary extracted from

  • Martel, A.; Masson, M.; Moreau, N.; Le Goffic, F.
    Kinetic studies of aminoglycoside acetyltransferase and phosphotransferase from Staphylococcus aureus RPAL. Relationship between the two activities (1983), Eur. J. Biochem., 133, 515-521.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.82 GTP, CoA, gentamicin C, neamine protect against thermal inactivation Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.82 6'-N-acetylneamine product inhibition Staphylococcus aureus
2.3.1.82 AMP
-
 Staphylococcus aureus
2.3.1.82 CoA product inhibition Staphylococcus aureus
2.3.1.82 gentamicin C1 competitive inhibitor of neamine, non-competitive inhibitor of acetyl-CoA Staphylococcus aureus
2.3.1.82 additional information no inhibition by GTP Staphylococcus aureus
2.3.1.82 paromomycin inhibits reaction with neamine Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.82 55000
-
 sucrose density gradient centrifugation Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.82 additional information Staphylococcus aureus involved in resistance to antibiotics ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Staphylococcus aureus
-
 harbouring plasmid RPAL
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.82 unseparable from 2-O-aminoglycoside phosphotransferase by affinity chromatography or sucrose density gradient ultracentrifugation Staphylococcus aureus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.82 acetyl-CoA + kanamycin-B = CoA + N6'-acetylkanamycin-B mechanism Staphylococcus aureus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.82 0.304
-
-
 Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 acetyl-CoA + gentamicin C1a
-
 Staphylococcus aureus CoA + N6'-acetylgentamicin C1a
-
 ?
2.3.1.82 acetyl-CoA + kanamycin B
-
 Staphylococcus aureus CoA + N6'-acetylkanamycin B
-
 ?
2.3.1.82 acetyl-CoA + neamine
-
 Staphylococcus aureus CoA + 6'-N-acetylneamine
-
 ?
2.3.1.82 acetyl-CoA + neamine
-
 Staphylococcus aureus CoA + N6'-acetylneamine
-
 ?
2.3.1.82 acetyl-CoA + tobramycin
-
 Staphylococcus aureus CoA + N6'-acetyltobramycin
-
 ?
2.3.1.82 additional information involved in resistance to antibiotics Staphylococcus aureus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.82 additional information
-
 GTP, CoA, gentamicin C, neamine protect against thermal inactivation Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.82 0.0036
-
 CoA pH 6.9, 37°C Staphylococcus aureus
2.3.1.82 4
-
 gentamicin C1 pH 6.9, 37°C, inhibition of the reaction with neamine Staphylococcus aureus
2.3.1.82 6
-
 AMP pH 6.9, 37°C, inhibition of the reaction with neamine Staphylococcus aureus
2.3.1.82 7.7
-
 paromomycin pH 6.9, 37°C, inhibition of the reaction with neamine Staphylococcus aureus