BRENDA - Enzyme Database

Partial purification and characterization of beta-mannosyltransferase from suspension-cultured soybean cells

Kaushal, G.P.; Elbein, A.D.; Biochemistry 26, 7953-7960 (1987)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.142
NP-40
-
Glycine max
2.4.1.142
phosphatidic acid
-
Glycine max
2.4.1.142
phosphatidylcholine
-
Glycine max
2.4.1.142
phosphatidylserine
-
Glycine max
2.4.1.142
Phospholipid
-
Glycine max
2.4.1.142
Triton X-100
optimum activity at 0.08-0.1%
Glycine max
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.142
GDP
-
Glycine max
2.4.1.142
GDP-glucose
-
Glycine max
2.4.1.142
GMP
-
Glycine max
2.4.1.142
GTP
-
Glycine max
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.142
0.0017
-
GDP-mannose
-
Glycine max
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.142
microsome
-
Glycine max
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.142
Mg2+
requirement for activity
Glycine max
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.142
Glycine max
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.142
DEAE-cellulose, GDP-affinity column
Glycine max
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.142
additional information
-
-
Glycine max
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.142
frozen, Tris-buffer, pH 7.0, 0.5 mM DTT, 20% glycerol, 0.1% detergent, 1 month
Glycine max
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.142
GDP-alpha-D-mannose + chitobiosyldiphosphodolichol
-
636890
Glycine max
GDP + beta-(1->4)-D-mannosylchitobiosyldiphosphodolichol
-
636890
Glycine max
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.142
6.9
7
-
Glycine max
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.142
NP-40
-
Glycine max
2.4.1.142
phosphatidic acid
-
Glycine max
2.4.1.142
phosphatidylcholine
-
Glycine max
2.4.1.142
phosphatidylserine
-
Glycine max
2.4.1.142
Phospholipid
-
Glycine max
2.4.1.142
Triton X-100
optimum activity at 0.08-0.1%
Glycine max
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.142
GDP
-
Glycine max
2.4.1.142
GDP-glucose
-
Glycine max
2.4.1.142
GMP
-
Glycine max
2.4.1.142
GTP
-
Glycine max
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.142
0.0017
-
GDP-mannose
-
Glycine max
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.142
microsome
-
Glycine max
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.142
Mg2+
requirement for activity
Glycine max
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.142
DEAE-cellulose, GDP-affinity column
Glycine max
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.142
additional information
-
-
Glycine max
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.142
frozen, Tris-buffer, pH 7.0, 0.5 mM DTT, 20% glycerol, 0.1% detergent, 1 month
Glycine max
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.142
GDP-alpha-D-mannose + chitobiosyldiphosphodolichol
-
636890
Glycine max
GDP + beta-(1->4)-D-mannosylchitobiosyldiphosphodolichol
-
636890
Glycine max
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.142
6.9
7
-
Glycine max