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Literature summary extracted from

  • Leal, N.A.; Park, S.D.; Kima, P.E.; Bobik, T.A.
    Identification of the human and bovine ATP:Cob(I)alamin adenosyltransferase cDNAs based on complementation of a bacterial mutant (2003), J. Biol. Chem., 278, 9227-9234.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.17 expressed in Escherichia coli Bos taurus
2.5.1.17 expressed in Escherichia coli Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.17 soluble from skin cells Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.17 K+
-
 Bos taurus
2.5.1.17 K+
-
 Homo sapiens
2.5.1.17 Mg2+
-
 Bos taurus
2.5.1.17 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.17 52000
-
 SDS-PAGE, recombinant enzyme without mitochondrial targeting sequence Homo sapiens
2.5.1.17 54000
-
 calculated from amino acid sequence of recombinant enzyme without mitochondrial targeting sequence Bos taurus
2.5.1.17 55000
-
 calculated from amino acid sequence of recombinant enzyme without mitochondrial targeting sequence Homo sapiens
2.5.1.17 56000
-
 SDS-PAGE, recombinant enzyme with mitochondrial targeting sequence Homo sapiens
2.5.1.17 58000
-
 calculated from amino acid sequence of recombinant enzyme with mitochondrial targeting sequence Bos taurus
2.5.1.17 58000
-
 calculated from amino acid sequence of recombinant enzyme with mitochondrial targeting sequence Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.17 Bos taurus
-
-
-
2.5.1.17 Homo sapiens Q96EY8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.17 centrifugation and SDS-PAGE Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.17 liver mRNA expression detected Bos taurus
-
2.5.1.17 liver mRNA expression detected Homo sapiens
-
2.5.1.17 skin skin fibroblasts show low abundance Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.17 additional information
-
 data for ATR inclusion bodies from the enzyme expressed in E. coli with no mitochondrial targeting sequence also available Bos taurus
2.5.1.17 0.047
-
 pH 8.0, 37°C Bos taurus
2.5.1.17 0.047
-
 bovine ATR containing mitochondrial targeting sequence, expressed in E. coli, soluble fraction Bos taurus
2.5.1.17 0.061
-
 pH 8.0, 37°C Homo sapiens
2.5.1.17 0.061
-
 human ATR containing mitochondrial targeting sequence, expressed in E. coli and forming inclusion bodies Homo sapiens
2.5.1.17 0.098
-
 pH 8.0, 37°C Homo sapiens
2.5.1.17 0.098
-
 human ATR with no mitochondrial targeting sequence, expressed in E. coli and forming inclusion bodies Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.17 ATP + cob(I)alamin
-
 Bos taurus triphosphate + coenzyme B12
-
 ?
2.5.1.17 ATP + cob(I)alamin
-
 Homo sapiens triphosphate + coenzyme B12
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.17 37
-
 assay at Bos taurus
2.5.1.17 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.17 8
-
 assay at Bos taurus
2.5.1.17 8
-
 assay at Homo sapiens