BRENDA - Enzyme Database

Purification and partial characterization of Cob(I)alamin adenosyltransferase from Pseudomonas denitrificans

Debussche, L.; Couder, M.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.; J. Bacteriol. 173, 6300-6302 (1991)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.5.1.17
DTT stabilizes during purification and storage, removal leads to complete loss of activity
Pseudomonas denitrificans (nomen rejiciendum)
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.17
hydrogenobyrinic acid a,c-diamide
cobalt-free analogue of cobyrinic acid a,c-diamide
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
additional information
no inhibition by cobyrinic acid, hydrogenobyrinic acid
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
tripolyphosphate
-
Pseudomonas denitrificans (nomen rejiciendum)
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.5.1.17
soluble
-
Pseudomonas denitrificans (nomen rejiciendum)
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.5.1.17
Mn2+
-
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.17
28000
-
2 * 28000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
56000
-
HPLC gel filtration
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
70000
-
PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.17
ATP + cob(I)alamin
Pseudomonas denitrificans (nomen rejiciendum)
-
triphosphate + coenzyme B12
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.17
Pseudomonas denitrificans (nomen rejiciendum)
P29930
recombinant strain SC510 Rifr containing plasmid pXL227, this DNA seuqence encodes for several enzymes
-
Purification (Commentary)
EC Number
Commentary
Organism
2.5.1.17
centrifugation, Mono Q chromatography and gel filtration
Pseudomonas denitrificans (nomen rejiciendum)
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.5.1.17
0.089
-
in the presence of cobalamin as substrate; pH 8.0, 30C
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
0.13
-
in the presence of cobyrinic acid a,c-diamide as substrate; pH 8.0, 30C
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
0.14
-
in the presence of cobyric acid as substrate; pH 8.0, 30C
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
0.18
-
in the presence of cobinamide as substrate; pH 8.0, 30C
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.17
ATP + cob(I)alamin
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
triphosphate + coenzyme B12
-
-
-
-
2.5.1.17
ATP + cob(I)alamin
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
triphosphate + coenzyme B12
adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12
636868
Pseudomonas denitrificans (nomen rejiciendum)
?
2.5.1.17
cob(I)inamide + ATP
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
5'-deoxy-5'-adenosyl-cob(I)inamide + polyphosphate
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
-
2.5.1.17
cob(I)yric acid + ATP
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
5'-deoxy-5'-adenosyl-cob(I)yric acid + polyphosphate
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
-
2.5.1.17
cob(I)yrinic acid a,c-diamide + ATP
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
5'-deoxy-5'-adenosyl-cob(I)yrinic acid a,c-diamide + polyphosphate
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
-
2.5.1.17
additional information
cobyrinic acid is not a substrate
636868
Pseudomonas denitrificans (nomen rejiciendum)
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.5.1.17
dimer
2 * 28000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.5.1.17
30
-
assay at
Pseudomonas denitrificans (nomen rejiciendum)
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.17
8
-
assay at
Pseudomonas denitrificans (nomen rejiciendum)
General Stability (protein specific)
EC Number
General Stability
Organism
2.5.1.17
DTT stabilizes during purification and storage, removal leads to complete loss of activity
Pseudomonas denitrificans (nomen rejiciendum)
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.17
hydrogenobyrinic acid a,c-diamide
cobalt-free analogue of cobyrinic acid a,c-diamide
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
additional information
no inhibition by cobyrinic acid, hydrogenobyrinic acid
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
tripolyphosphate
-
Pseudomonas denitrificans (nomen rejiciendum)
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.5.1.17
soluble
-
Pseudomonas denitrificans (nomen rejiciendum)
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.5.1.17
Mn2+
-
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.17
28000
-
2 * 28000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
56000
-
HPLC gel filtration
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
70000
-
PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.17
ATP + cob(I)alamin
Pseudomonas denitrificans (nomen rejiciendum)
-
triphosphate + coenzyme B12
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.17
centrifugation, Mono Q chromatography and gel filtration
Pseudomonas denitrificans (nomen rejiciendum)
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.5.1.17
0.089
-
in the presence of cobalamin as substrate; pH 8.0, 30C
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
0.13
-
in the presence of cobyrinic acid a,c-diamide as substrate; pH 8.0, 30C
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
0.14
-
in the presence of cobyric acid as substrate; pH 8.0, 30C
Pseudomonas denitrificans (nomen rejiciendum)
2.5.1.17
0.18
-
in the presence of cobinamide as substrate; pH 8.0, 30C
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.17
ATP + cob(I)alamin
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
triphosphate + coenzyme B12
-
-
-
-
2.5.1.17
ATP + cob(I)alamin
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
triphosphate + coenzyme B12
adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12
636868
Pseudomonas denitrificans (nomen rejiciendum)
?
2.5.1.17
cob(I)inamide + ATP
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
5'-deoxy-5'-adenosyl-cob(I)inamide + polyphosphate
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
-
2.5.1.17
cob(I)yric acid + ATP
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
5'-deoxy-5'-adenosyl-cob(I)yric acid + polyphosphate
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
-
2.5.1.17
cob(I)yrinic acid a,c-diamide + ATP
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
5'-deoxy-5'-adenosyl-cob(I)yrinic acid a,c-diamide + polyphosphate
-
636868
Pseudomonas denitrificans (nomen rejiciendum)
-
2.5.1.17
additional information
cobyrinic acid is not a substrate
636868
Pseudomonas denitrificans (nomen rejiciendum)
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.5.1.17
dimer
2 * 28000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.5.1.17
30
-
assay at
Pseudomonas denitrificans (nomen rejiciendum)
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.17
8
-
assay at
Pseudomonas denitrificans (nomen rejiciendum)