EC Number | General Stability | Organism |
---|---|---|
2.5.1.17 | 2-mercaptoethanol stabilizes during purification | Clostridium tetanomorphum |
2.5.1.17 | 2-mercaptoethanol stabilizes during purification | Lactobacillus leichmannii |
2.5.1.17 | repeated freeze-thawing inactivates | Clostridium tetanomorphum |
2.5.1.17 | repeated freeze-thawing inactivates | Lactobacillus leichmannii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.17 | Ca2+ | - |
Lactobacillus leichmannii | |
2.5.1.17 | Cd2+ | - |
Lactobacillus leichmannii | |
2.5.1.17 | diphosphate | - |
Clostridium tetanomorphum | |
2.5.1.17 | diphosphate | - |
Lactobacillus leichmannii | |
2.5.1.17 | Mn2+ | above 10 mM | Lactobacillus leichmannii | |
2.5.1.17 | tripolyphosphate | - |
Clostridium tetanomorphum | |
2.5.1.17 | tripolyphosphate | - |
Lactobacillus leichmannii | |
2.5.1.17 | Zn2+ | - |
Lactobacillus leichmannii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.17 | 1.3 | - |
ATP | pH 8.0, 37°C | Lactobacillus leichmannii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.5.1.17 | additional information | subcellular distribution | Lactobacillus leichmannii | - |
- |
2.5.1.17 | ribosome | - |
Lactobacillus delbrueckii | 5840 | - |
2.5.1.17 | ribosome | - |
Lactobacillus leichmannii | 5840 | - |
2.5.1.17 | soluble | - |
Clostridium tetanomorphum | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.17 | Cs+ | activation | Lactobacillus leichmannii | |
2.5.1.17 | K+ | - |
Clostridium tetanomorphum | |
2.5.1.17 | K+ | activation | Lactobacillus leichmannii | |
2.5.1.17 | K+ | may be acting upon a step in the reduction of hydroxocobamide | Propionibacterium freudenreichii subsp. shermanii | |
2.5.1.17 | Mg2+ | - |
Propionibacterium freudenreichii subsp. shermanii | |
2.5.1.17 | Mg2+ | activation, less effective than Mn2+ | Clostridium tetanomorphum | |
2.5.1.17 | Mg2+ | activation, less effective than Mn2+ | Lactobacillus leichmannii | |
2.5.1.17 | Mn2+ | - |
Clostridium tetanomorphum | |
2.5.1.17 | Mn2+ | - |
Lactobacillus leichmannii | |
2.5.1.17 | Na+ | activation | Lactobacillus leichmannii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.17 | ATP + cob(I)alamin | Propionibacterium freudenreichii subsp. shermanii | - |
triphosphate + coenzyme B12 | - |
? | |
2.5.1.17 | ATP + cob(I)alamin | Lactobacillus delbrueckii | - |
triphosphate + coenzyme B12 | - |
? | |
2.5.1.17 | ATP + cob(I)alamin | Clostridium tetanomorphum | - |
triphosphate + coenzyme B12 | - |
? | |
2.5.1.17 | ATP + cob(I)alamin | Lactobacillus leichmannii | - |
triphosphate + coenzyme B12 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.17 | Clostridium tetanomorphum | - |
- |
- |
2.5.1.17 | Lactobacillus delbrueckii | - |
- |
- |
2.5.1.17 | Lactobacillus leichmannii | - |
- |
- |
2.5.1.17 | Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.17 | partial purification of the enzyme is achieved from ribosomal fraction by salt extraction and ammonium sulfate fractionation, followed by two chromatographic steps | Lactobacillus leichmannii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.17 | 0.000125 | - |
activity measured using washed ribosomes | Lactobacillus leichmannii |
2.5.1.17 | 0.000125 | - |
pH 8.0, 37°C | Lactobacillus leichmannii |
EC Number | Storage Stability | Organism |
---|---|---|
2.5.1.17 | -20°C, more than 50% loss of activity within 1 week, with 50% glycerol only 20% loss of activity | Lactobacillus leichmannii |
2.5.1.17 | 2-mercaptoethanol stabilizes during storage | Clostridium tetanomorphum |
2.5.1.17 | 2-mercaptoethanol stabilizes during storage | Lactobacillus leichmannii |
2.5.1.17 | DTT stabilizes during storage | Lactobacillus leichmannii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.17 | ATP + cob(I)alamin | - |
Propionibacterium freudenreichii subsp. shermanii | triphosphate + coenzyme B12 | - |
? | |
2.5.1.17 | ATP + cob(I)alamin | - |
Lactobacillus delbrueckii | triphosphate + coenzyme B12 | - |
? | |
2.5.1.17 | ATP + cob(I)alamin | - |
Clostridium tetanomorphum | triphosphate + coenzyme B12 | - |
? | |
2.5.1.17 | ATP + cob(I)alamin | - |
Lactobacillus leichmannii | triphosphate + coenzyme B12 | - |
? | |
2.5.1.17 | ATP + cob(I)alamin | - |
Propionibacterium freudenreichii subsp. shermanii | triphosphate + coenzyme B12 | adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 | ? | |
2.5.1.17 | ATP + cob(I)alamin | - |
Lactobacillus delbrueckii | triphosphate + coenzyme B12 | adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 | ? | |
2.5.1.17 | ATP + cob(I)alamin | - |
Clostridium tetanomorphum | triphosphate + coenzyme B12 | adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 | ? | |
2.5.1.17 | ATP + cob(I)alamin | - |
Lactobacillus leichmannii | triphosphate + coenzyme B12 | adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 | ? | |
2.5.1.17 | additional information | - |
Propionibacterium freudenreichii subsp. shermanii | ? | - |
? | |
2.5.1.17 | additional information | - |
Clostridium tetanomorphum | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.17 | 37 | - |
assay at | Clostridium tetanomorphum |
2.5.1.17 | 37 | - |
assay at | Lactobacillus leichmannii |