BRENDA - Enzyme Database

Some molecular and enzymatic properties of a homogeneous preparation of thiaminase I purified from carp liver

Bos, M.; Kozik, A.; J. Protein Chem. 19, 75-84 (2000) View publication on PubMed

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.5.1.2
cell associated
-
Cyprinus carpio
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.2
55000
-
gel filtration
Cyprinus carpio
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.5.1.2
Cyprinus carpio
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.5.1.2
by ammonium precipitation, CM-cellulose column, DEAE-cellulose column, Cibacron blue Sepharose column, Sephacryl S-300 column, Superdex-200 and Superdex-75 column
Cyprinus carpio
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.5.1.2
liver
-
Cyprinus carpio
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.5.1.2
1'-methylthiamine + aniline
22% of activity with thiamine
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl)-thiazole + 4-amino-5-(anilinomethyl)-1,2-dimethylpyrimidin-1-ium
-
-
-
?
2.5.1.2
2'-ethylthiamine + aniline
63% of activity with thiamine
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(anilinomethyl)-2-ethylpyrimidin-4-amine
-
-
-
?
2.5.1.2
2-(1-hydroxyethyl)thiamine + aniline
92% of activity with thiamine
636860
Cyprinus carpio
4-methyl-2-(1-hydroxyethyl)-5-(2-hydroxyethyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
5-chloroethylthiamine + aniline
67% of activity with thiamine
636860
Cyprinus carpio
4-methyl-5-chloroethyl-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
5-nor-thiamine + aniline
88% of activity with thiamine
636860
Cyprinus carpio
4-methyl-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
additional information
no activity with 4'-deaminothiamine and 4'-oxythiamine suggest that 4'-amino group is essential for enzyme activity
636860
Cyprinus carpio
?
-
-
-
?
2.5.1.2
O-(beta-D-galactosyl)thiamine + aniline
63% of activity with thiamine
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl-O-beta-D-galactosyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
O-benzoylthiamine + aniline
91% of activity with thiamine
636860
Cyprinus carpio
2-(4-methyl-1,3-thiazol-5-yl)ethyl benzoate + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
O-S-diacetylthiamine + aniline
50% of activity with thiamine
636860
Cyprinus carpio
1-acetyl-4-methyl-5-(2-hydroxyethyl-O-acetyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
O-succinylthiamine + aniline
83% of activity with thiamine
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl-O-succinyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
pyrithiamine + aniline
72% of activity with thiamine
636860
Cyprinus carpio
2-(2-methylpyridin-3-yl)ethanol + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
tetrahydrothiamine + aniline
84% of activity with thiamine
636860
Cyprinus carpio
?
-
-
-
?
2.5.1.2
thiamine + 2-mercaptoethanol
63% of activity with aniline
636860
Cyprinus carpio
?
-
-
-
?
2.5.1.2
thiamine + aniline
taken as 100% of activity of purified enzyme
636860
Cyprinus carpio
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636860
Cyprinus carpio
?
2.5.1.2
thiamine + imidazole
24% of activity with aniline
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
thiamine + L-cysteine
50% of activity with aniline
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid
-
-
-
?
2.5.1.2
thiamine + pyridine
best substrate with aniline
636860
Cyprinus carpio
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636860
Cyprinus carpio
?
2.5.1.2
thiamine disulfide + aniline
26% of activity with thiamine
636860
Cyprinus carpio
?
-
-
-
?
2.5.1.2
thiamine phosphate + aniline
64% of activity with thiamine
636860
Cyprinus carpio
2-(4-methyl-1,3-thiazol-5-yl)ethyl dihydrogenphosphate + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
thiothiamine + aniline
12% of activity with thiamine
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl)-2-thio-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.2
40
-
-
Cyprinus carpio
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.2
6
7
-
Cyprinus carpio
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.5.1.2
cell associated
-
Cyprinus carpio
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.2
55000
-
gel filtration
Cyprinus carpio
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.2
by ammonium precipitation, CM-cellulose column, DEAE-cellulose column, Cibacron blue Sepharose column, Sephacryl S-300 column, Superdex-200 and Superdex-75 column
Cyprinus carpio
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.5.1.2
liver
-
Cyprinus carpio
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.5.1.2
1'-methylthiamine + aniline
22% of activity with thiamine
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl)-thiazole + 4-amino-5-(anilinomethyl)-1,2-dimethylpyrimidin-1-ium
-
-
-
?
2.5.1.2
2'-ethylthiamine + aniline
63% of activity with thiamine
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(anilinomethyl)-2-ethylpyrimidin-4-amine
-
-
-
?
2.5.1.2
2-(1-hydroxyethyl)thiamine + aniline
92% of activity with thiamine
636860
Cyprinus carpio
4-methyl-2-(1-hydroxyethyl)-5-(2-hydroxyethyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
5-chloroethylthiamine + aniline
67% of activity with thiamine
636860
Cyprinus carpio
4-methyl-5-chloroethyl-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
5-nor-thiamine + aniline
88% of activity with thiamine
636860
Cyprinus carpio
4-methyl-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
additional information
no activity with 4'-deaminothiamine and 4'-oxythiamine suggest that 4'-amino group is essential for enzyme activity
636860
Cyprinus carpio
?
-
-
-
?
2.5.1.2
O-(beta-D-galactosyl)thiamine + aniline
63% of activity with thiamine
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl-O-beta-D-galactosyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
O-benzoylthiamine + aniline
91% of activity with thiamine
636860
Cyprinus carpio
2-(4-methyl-1,3-thiazol-5-yl)ethyl benzoate + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
O-S-diacetylthiamine + aniline
50% of activity with thiamine
636860
Cyprinus carpio
1-acetyl-4-methyl-5-(2-hydroxyethyl-O-acetyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
O-succinylthiamine + aniline
83% of activity with thiamine
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl-O-succinyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
pyrithiamine + aniline
72% of activity with thiamine
636860
Cyprinus carpio
2-(2-methylpyridin-3-yl)ethanol + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
tetrahydrothiamine + aniline
84% of activity with thiamine
636860
Cyprinus carpio
?
-
-
-
?
2.5.1.2
thiamine + 2-mercaptoethanol
63% of activity with aniline
636860
Cyprinus carpio
?
-
-
-
?
2.5.1.2
thiamine + aniline
taken as 100% of activity of purified enzyme
636860
Cyprinus carpio
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636860
Cyprinus carpio
?
2.5.1.2
thiamine + imidazole
24% of activity with aniline
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
thiamine + L-cysteine
50% of activity with aniline
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid
-
-
-
?
2.5.1.2
thiamine + pyridine
best substrate with aniline
636860
Cyprinus carpio
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636860
Cyprinus carpio
?
2.5.1.2
thiamine disulfide + aniline
26% of activity with thiamine
636860
Cyprinus carpio
?
-
-
-
?
2.5.1.2
thiamine phosphate + aniline
64% of activity with thiamine
636860
Cyprinus carpio
2-(4-methyl-1,3-thiazol-5-yl)ethyl dihydrogenphosphate + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
thiothiamine + aniline
12% of activity with thiamine
636860
Cyprinus carpio
4-methyl-5-(2-hydroxyethyl)-2-thio-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.2
40
-
-
Cyprinus carpio
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.2
6
7
-
Cyprinus carpio