BRENDA - Enzyme Database

The purification and properties of a thiaminase I enzyme from nardoo (Marsilea Drumondii)

McCleary, B.V.; Chick, B.F.; Phytochemistry 16, 207-213 (1977)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.5.1.2
2-mercaptoethanol
complete reactivation at 3.3 mM but inhibition at 33 mM
Marsilea drummondii
2.5.1.2
cysteine
complete reactivation at 3.3 mM but inhibition at 33 mM
Marsilea drummondii
2.5.1.2
Thioglycollate
greater reactivation at 33 mM than at 3.3 mM
Marsilea drummondii
2.5.1.2
thiosulphate
greater reactivation at 33 mM than at 3.3 mM
Marsilea drummondii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.2
Ag+
100% inhibition at 1 mM
Marsilea drummondii
2.5.1.2
Cu2+
50% of inhibition at 1 mM
Marsilea drummondii
2.5.1.2
Fe2+
70% of inhibition at 1 mM
Marsilea drummondii
2.5.1.2
HgCl2
potent inhibitor at 0.001 mM
Cheilanthes sieberi
2.5.1.2
HgCl2
potent inhibitor at 0.001 mM
Marsilea drummondii
2.5.1.2
HgCl2
potent inhibitor at 0.001 mM
Pteridium esculentum
2.5.1.2
HgCl2
potent inhibitor at 0.001 mM
Velesunio ambiguus
2.5.1.2
Mersalyl acid
potent inhibitor at 0.001 mM
Cheilanthes sieberi
2.5.1.2
Mersalyl acid
potent inhibitor at 0.001 mM
Marsilea drummondii
2.5.1.2
Mersalyl acid
potent inhibitor at 0.001 mM
Pteridium esculentum
2.5.1.2
Mersalyl acid
potent inhibitor at 0.001 mM
Velesunio ambiguus
2.5.1.2
Mn2+
no inhibition
Marsilea drummondii
2.5.1.2
additional information
not inhibited by EDTA
Marsilea drummondii
2.5.1.2
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Cheilanthes sieberi
2.5.1.2
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Marsilea drummondii
2.5.1.2
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Pteridium esculentum
2.5.1.2
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Velesunio ambiguus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.2
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Marsilea drummondii
2.5.1.2
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Pteridium esculentum
2.5.1.2
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Cheilanthes sieberi
2.5.1.2
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Velesunio ambiguus
2.5.1.2
0.5
-
beta-picoline
-
Marsilea drummondii
2.5.1.2
0.7
-
Pyridine
-
Pteridium esculentum
2.5.1.2
0.7
-
Pyridine
-
Velesunio ambiguus
2.5.1.2
0.9
-
nicotinic acid
-
Velesunio ambiguus
2.5.1.2
0.9
-
gamma-Picoline
-
Marsilea drummondii
2.5.1.2
1.4
-
aniline
lower maximal activity than pyridine and nicotinic acid
Marsilea drummondii
2.5.1.2
1.4
-
aniline
lower maximal activity than pyridine and nicotinic acid
Pteridium esculentum
2.5.1.2
1.7
-
m-phenylene diamine
-
Marsilea drummondii
2.5.1.2
1.7
-
o-phenylene diamine
-
Marsilea drummondii
2.5.1.2
2
-
aniline
lower maximal activity than pyridine and nicotinic acid
Velesunio ambiguus
2.5.1.2
2.4
-
m-Toluidine
-
Marsilea drummondii
2.5.1.2
2.5
-
Pyridine
-
Marsilea drummondii
2.5.1.2
2.8
-
3-hydroxyaniline
-
Marsilea drummondii
2.5.1.2
2.9
-
3-aminopyridine
-
Marsilea drummondii
2.5.1.2
3.3
-
Quinoline
-
Marsilea drummondii
2.5.1.2
3.3
-
alpha-picoline
-
Marsilea drummondii
2.5.1.2
3.3
-
p-phenylene diamine
-
Marsilea drummondii
2.5.1.2
3.4
-
gamma-picolinic acid
-
Marsilea drummondii
2.5.1.2
4.2
-
2-hydroxyaniline
-
Marsilea drummondii
2.5.1.2
5
-
Pyridine
-
Cheilanthes sieberi
2.5.1.2
5
-
o-mercaptobenzoic acid
best cosubstrate
Marsilea drummondii
2.5.1.2
5.1
-
aniline
lower maximal activity than pyridine and nicotinic acid
Cheilanthes sieberi
2.5.1.2
6.4
-
o-toluidine
-
Marsilea drummondii
2.5.1.2
9.5
-
adenine
-
Marsilea drummondii
2.5.1.2
12.5
-
2-aminopyridine
-
Marsilea drummondii
2.5.1.2
15.4
-
4-aminopyridine
-
Marsilea drummondii
2.5.1.2
20
-
4-Hydroxyaniline
-
Marsilea drummondii
2.5.1.2
21.2
-
alpha-picolinic acid
-
Marsilea drummondii
2.5.1.2
25
-
nicotinic acid
-
Marsilea drummondii
2.5.1.2
25
-
beta-picolinic acid
-
Marsilea drummondii
2.5.1.2
29
-
nicotinic acid
-
Pteridium esculentum
2.5.1.2
32
-
methylamine
-
Marsilea drummondii
2.5.1.2
33
-
L-cysteine
-
Marsilea drummondii
2.5.1.2
37
-
o-aminobenzoic acid
-
Marsilea drummondii
2.5.1.2
37
-
m-aminobenzoic acid
-
Marsilea drummondii
2.5.1.2
38
-
6-aminohexanoic acid
-
Marsilea drummondii
2.5.1.2
50
-
Sulfanilic acid
-
Marsilea drummondii
2.5.1.2
50
-
3-nitroaniline
-
Marsilea drummondii
2.5.1.2
55
-
nicotinic acid
-
Cheilanthes sieberi
2.5.1.2
77
-
sulfanilamide
-
Marsilea drummondii
2.5.1.2
111
-
proline
-
Marsilea drummondii
2.5.1.2
125
-
imidazole
-
Marsilea drummondii
2.5.1.2
200
-
Tris
-
Marsilea drummondii
2.5.1.2
200
-
p-Aminobenzoic acid
-
Marsilea drummondii
2.5.1.2
400
-
hydroxyproline
-
Marsilea drummondii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.5.1.2
additional information
Ca2+, Mo+, Mn2+, Zn2+, Mg2+ have no effect on activity
Marsilea drummondii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.2
93000
-
gel filtration
Pteridium esculentum
2.5.1.2
107000
-
gel filtration
Cheilanthes sieberi
2.5.1.2
110000
-
gel filtration
Velesunio ambiguus
2.5.1.2
115000
-
gel filtration
Marsilea drummondii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.5.1.2
thiamine + adenine
Marsilea drummondii
-
?
-
-
?
2.5.1.2
thiamine + hydroxyproline
Marsilea drummondii
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
?
2.5.1.2
thiamine + hydroxyproline
Pteridium esculentum
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
?
2.5.1.2
thiamine + proline
Marsilea drummondii
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
?
2.5.1.2
thiamine + proline
Pteridium esculentum
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.5.1.2
Cheilanthes sieberi
-
rock fern
-
2.5.1.2
Marsilea angustifolia
-
-
-
2.5.1.2
Marsilea drummondii
-
nardoo
-
2.5.1.2
Marsilea mutica
-
-
-
2.5.1.2
Pteridium esculentum
-
bracken fern
-
2.5.1.2
Velesunio ambiguus
-
freshwater mussel
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.5.1.2
by DEAE-cellulose chromatrography, ultrogel AcA 34 column, Sephadex G-100 column and phosphocellulose column
Marsilea drummondii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.5.1.2
frond
extremely high levels of the enzyme, lush fronds have much more enzyme than old fronds
Marsilea drummondii
-
2.5.1.2
frond
activity found in lush fronds
Marsilea mutica
-
2.5.1.2
frond
activity found in lush fronds
Cheilanthes sieberi
-
2.5.1.2
frond
very low activity in lush fronds
Marsilea angustifolia
-
2.5.1.2
frond
very low activity in youngs and old fronds
Pteridium esculentum
-
2.5.1.2
root
lush growth
Marsilea drummondii
-
2.5.1.2
tuber
very low activity
Pteridium esculentum
-
2.5.1.2
whole body
activity found
Velesunio ambiguus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.5.1.2
thiamine + 2-aminopyridine
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + 2-hydroxyaniline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-hydroxyphenyl)amine
-
-
-
?
2.5.1.2
thiamine + 2-mercaptobenzoic acid
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + 2-methylbenzeneamine
i.e. o-toluidine
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-methylphenyl)amine
-
-
-
?
2.5.1.2
thiamine + 2-pyridinecarboxylic acid
i.e. alpha-picolinic acid
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxypyridinium
-
-
-
?
2.5.1.2
thiamine + 3-aminopyridine
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + 3-hydroxyaniline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-hydroxyphenyl)amine
-
-
-
?
2.5.1.2
thiamine + 3-methylbenzeneamine
i.e. m-toluidine
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-methylphenyl)amine
-
-
-
?
2.5.1.2
thiamine + 3-nitroaniline
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + 3-pyridinecarboxylic acid
i.e. beta-picolinic acid
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-carboxypyridinium
-
-
-
?
2.5.1.2
thiamine + 4-aminopyridine
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + 4-hydroxyaniline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-hydroxyphenyl)amine
-
-
-
?
2.5.1.2
thiamine + 4-methylbenzeneamine
i.e. p-toluidine
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-methylphenyl)amine
-
-
-
?
2.5.1.2
thiamine + 4-pyridinecarboxylic acid
i.e. gamma-picolinic acid
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-carboxypyridinium
-
-
-
?
2.5.1.2
thiamine + 6-aminohexanoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 6-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]hexanoic acid
-
-
-
?
2.5.1.2
thiamine + adenine
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + alpha-picoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]alpha-picolinium
-
-
-
?
2.5.1.2
thiamine + aniline
-
636851
Marsilea drummondii
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Marsilea drummondii
?
2.5.1.2
thiamine + aniline
-
636851
Pteridium esculentum
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Pteridium esculentum
?
2.5.1.2
thiamine + aniline
-
636851
Cheilanthes sieberi
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Cheilanthes sieberi
?
2.5.1.2
thiamine + aniline
-
636851
Velesunio ambiguus
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Velesunio ambiguus
?
2.5.1.2
thiamine + beta-picoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]beta-picolinium
-
-
-
?
2.5.1.2
thiamine + gamma-picoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]gamma-picolinium
-
-
-
?
2.5.1.2
thiamine + hydroxyproline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
-
?
2.5.1.2
thiamine + hydroxyproline
-
636851
Pteridium esculentum
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
-
?
2.5.1.2
thiamine + imidazole
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
thiamine + L-cysteine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid
-
-
-
?
2.5.1.2
thiamine + m-aminobenzoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 3-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
-
-
?
2.5.1.2
thiamine + m-phenylene diamine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-aminophenyl)amine
-
-
-
?
2.5.1.2
thiamine + methylamine
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + nicotinic acid
-
636851
Pteridium esculentum
?
-
-
-
?
2.5.1.2
thiamine + nicotinic acid
-
636851
Cheilanthes sieberi
?
-
-
-
?
2.5.1.2
thiamine + nicotinic acid
-
636851
Velesunio ambiguus
?
-
-
-
?
2.5.1.2
thiamine + o-aminobenzoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
-
-
?
2.5.1.2
thiamine + o-phenylene diamine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-aminophenyl)amine
-
-
-
?
2.5.1.2
thiamine + p-aminobenzoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 4-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
-
-
?
2.5.1.2
thiamine + p-phenylene diamine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-aminophenyl)amine
-
-
-
?
2.5.1.2
thiamine + proline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
-
?
2.5.1.2
thiamine + proline
-
636851
Pteridium esculentum
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
-
?
2.5.1.2
thiamine + pyridine
-
636851
Marsilea drummondii
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Marsilea drummondii
?
2.5.1.2
thiamine + pyridine
-
636851
Pteridium esculentum
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Pteridium esculentum
?
2.5.1.2
thiamine + pyridine
-
636851
Cheilanthes sieberi
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Cheilanthes sieberi
?
2.5.1.2
thiamine + pyridine
-
636851
Velesunio ambiguus
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Velesunio ambiguus
?
2.5.1.2
thiamine + quinoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium
-
-
-
?
2.5.1.2
thiamine + sulfanilamide
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + sulfanilic acid
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + Tris
-
636851
Marsilea drummondii
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.2
8
9
-
Marsilea drummondii
2.5.1.2
8
9
-
Pteridium esculentum
2.5.1.2
8
9
-
Cheilanthes sieberi
2.5.1.2
8
9
-
Velesunio ambiguus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.5.1.2
3
12
-
Marsilea drummondii
2.5.1.2
3
12
-
Pteridium esculentum
2.5.1.2
3
12
-
Cheilanthes sieberi
2.5.1.2
3
12
-
Velesunio ambiguus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.5.1.2
3
12
stable in this range
Marsilea drummondii
2.5.1.2
3
12
stable in this range
Pteridium esculentum
2.5.1.2
3
12
stable in this range
Cheilanthes sieberi
2.5.1.2
3
12
stable in this range
Velesunio ambiguus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.5.1.2
2-mercaptoethanol
complete reactivation at 3.3 mM but inhibition at 33 mM
Marsilea drummondii
2.5.1.2
cysteine
complete reactivation at 3.3 mM but inhibition at 33 mM
Marsilea drummondii
2.5.1.2
Thioglycollate
greater reactivation at 33 mM than at 3.3 mM
Marsilea drummondii
2.5.1.2
thiosulphate
greater reactivation at 33 mM than at 3.3 mM
Marsilea drummondii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.2
Ag+
100% inhibition at 1 mM
Marsilea drummondii
2.5.1.2
Cu2+
50% of inhibition at 1 mM
Marsilea drummondii
2.5.1.2
Fe2+
70% of inhibition at 1 mM
Marsilea drummondii
2.5.1.2
HgCl2
potent inhibitor at 0.001 mM
Marsilea drummondii
2.5.1.2
HgCl2
potent inhibitor at 0.001 mM
Pteridium esculentum
2.5.1.2
HgCl2
potent inhibitor at 0.001 mM
Cheilanthes sieberi
2.5.1.2
HgCl2
potent inhibitor at 0.001 mM
Velesunio ambiguus
2.5.1.2
Mersalyl acid
potent inhibitor at 0.001 mM
Marsilea drummondii
2.5.1.2
Mersalyl acid
potent inhibitor at 0.001 mM
Pteridium esculentum
2.5.1.2
Mersalyl acid
potent inhibitor at 0.001 mM
Cheilanthes sieberi
2.5.1.2
Mersalyl acid
potent inhibitor at 0.001 mM
Velesunio ambiguus
2.5.1.2
Mn2+
no inhibition
Marsilea drummondii
2.5.1.2
additional information
not inhibited by EDTA
Marsilea drummondii
2.5.1.2
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Marsilea drummondii
2.5.1.2
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Pteridium esculentum
2.5.1.2
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Cheilanthes sieberi
2.5.1.2
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Velesunio ambiguus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.2
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Marsilea drummondii
2.5.1.2
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Pteridium esculentum
2.5.1.2
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Cheilanthes sieberi
2.5.1.2
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Velesunio ambiguus
2.5.1.2
0.5
-
beta-picoline
-
Marsilea drummondii
2.5.1.2
0.7
-
Pyridine
-
Pteridium esculentum
2.5.1.2
0.7
-
Pyridine
-
Velesunio ambiguus
2.5.1.2
0.9
-
nicotinic acid
-
Velesunio ambiguus
2.5.1.2
0.9
-
gamma-Picoline
-
Marsilea drummondii
2.5.1.2
1.4
-
aniline
lower maximal activity than pyridine and nicotinic acid
Marsilea drummondii
2.5.1.2
1.4
-
aniline
lower maximal activity than pyridine and nicotinic acid
Pteridium esculentum
2.5.1.2
1.7
-
m-phenylene diamine
-
Marsilea drummondii
2.5.1.2
1.7
-
o-phenylene diamine
-
Marsilea drummondii
2.5.1.2
2
-
aniline
lower maximal activity than pyridine and nicotinic acid
Velesunio ambiguus
2.5.1.2
2.4
-
m-Toluidine
-
Marsilea drummondii
2.5.1.2
2.5
-
Pyridine
-
Marsilea drummondii
2.5.1.2
2.8
-
3-hydroxyaniline
-
Marsilea drummondii
2.5.1.2
2.9
-
3-aminopyridine
-
Marsilea drummondii
2.5.1.2
3.3
-
Quinoline
-
Marsilea drummondii
2.5.1.2
3.3
-
alpha-picoline
-
Marsilea drummondii
2.5.1.2
3.3
-
p-phenylene diamine
-
Marsilea drummondii
2.5.1.2
3.4
-
gamma-picolinic acid
-
Marsilea drummondii
2.5.1.2
4.2
-
2-hydroxyaniline
-
Marsilea drummondii
2.5.1.2
5
-
Pyridine
-
Cheilanthes sieberi
2.5.1.2
5
-
o-mercaptobenzoic acid
best cosubstrate
Marsilea drummondii
2.5.1.2
5.1
-
aniline
lower maximal activity than pyridine and nicotinic acid
Cheilanthes sieberi
2.5.1.2
6.4
-
o-toluidine
-
Marsilea drummondii
2.5.1.2
9.5
-
adenine
-
Marsilea drummondii
2.5.1.2
12.5
-
2-aminopyridine
-
Marsilea drummondii
2.5.1.2
15.4
-
4-aminopyridine
-
Marsilea drummondii
2.5.1.2
20
-
4-Hydroxyaniline
-
Marsilea drummondii
2.5.1.2
21.2
-
alpha-picolinic acid
-
Marsilea drummondii
2.5.1.2
25
-
nicotinic acid
-
Marsilea drummondii
2.5.1.2
25
-
beta-picolinic acid
-
Marsilea drummondii
2.5.1.2
29
-
nicotinic acid
-
Pteridium esculentum
2.5.1.2
32
-
methylamine
-
Marsilea drummondii
2.5.1.2
33
-
L-cysteine
-
Marsilea drummondii
2.5.1.2
37
-
o-aminobenzoic acid
-
Marsilea drummondii
2.5.1.2
37
-
m-aminobenzoic acid
-
Marsilea drummondii
2.5.1.2
38
-
6-aminohexanoic acid
-
Marsilea drummondii
2.5.1.2
50
-
Sulfanilic acid
-
Marsilea drummondii
2.5.1.2
50
-
3-nitroaniline
-
Marsilea drummondii
2.5.1.2
55
-
nicotinic acid
-
Cheilanthes sieberi
2.5.1.2
77
-
sulfanilamide
-
Marsilea drummondii
2.5.1.2
111
-
proline
-
Marsilea drummondii
2.5.1.2
125
-
imidazole
-
Marsilea drummondii
2.5.1.2
200
-
Tris
-
Marsilea drummondii
2.5.1.2
200
-
p-Aminobenzoic acid
-
Marsilea drummondii
2.5.1.2
400
-
hydroxyproline
-
Marsilea drummondii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.5.1.2
additional information
Ca2+, Mo+, Mn2+, Zn2+, Mg2+ have no effect on activity
Marsilea drummondii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.2
93000
-
gel filtration
Pteridium esculentum
2.5.1.2
107000
-
gel filtration
Cheilanthes sieberi
2.5.1.2
110000
-
gel filtration
Velesunio ambiguus
2.5.1.2
115000
-
gel filtration
Marsilea drummondii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.5.1.2
thiamine + adenine
Marsilea drummondii
-
?
-
-
?
2.5.1.2
thiamine + hydroxyproline
Marsilea drummondii
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
?
2.5.1.2
thiamine + hydroxyproline
Pteridium esculentum
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
?
2.5.1.2
thiamine + proline
Marsilea drummondii
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
?
2.5.1.2
thiamine + proline
Pteridium esculentum
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.2
by DEAE-cellulose chromatrography, ultrogel AcA 34 column, Sephadex G-100 column and phosphocellulose column
Marsilea drummondii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.5.1.2
frond
extremely high levels of the enzyme, lush fronds have much more enzyme than old fronds
Marsilea drummondii
-
2.5.1.2
frond
activity found in lush fronds
Marsilea mutica
-
2.5.1.2
frond
activity found in lush fronds
Cheilanthes sieberi
-
2.5.1.2
frond
very low activity in lush fronds
Marsilea angustifolia
-
2.5.1.2
frond
very low activity in youngs and old fronds
Pteridium esculentum
-
2.5.1.2
root
lush growth
Marsilea drummondii
-
2.5.1.2
tuber
very low activity
Pteridium esculentum
-
2.5.1.2
whole body
activity found
Velesunio ambiguus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.5.1.2
thiamine + 2-aminopyridine
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + 2-hydroxyaniline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-hydroxyphenyl)amine
-
-
-
?
2.5.1.2
thiamine + 2-mercaptobenzoic acid
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + 2-methylbenzeneamine
i.e. o-toluidine
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-methylphenyl)amine
-
-
-
?
2.5.1.2
thiamine + 2-pyridinecarboxylic acid
i.e. alpha-picolinic acid
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxypyridinium
-
-
-
?
2.5.1.2
thiamine + 3-aminopyridine
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + 3-hydroxyaniline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-hydroxyphenyl)amine
-
-
-
?
2.5.1.2
thiamine + 3-methylbenzeneamine
i.e. m-toluidine
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-methylphenyl)amine
-
-
-
?
2.5.1.2
thiamine + 3-nitroaniline
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + 3-pyridinecarboxylic acid
i.e. beta-picolinic acid
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-carboxypyridinium
-
-
-
?
2.5.1.2
thiamine + 4-aminopyridine
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + 4-hydroxyaniline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-hydroxyphenyl)amine
-
-
-
?
2.5.1.2
thiamine + 4-methylbenzeneamine
i.e. p-toluidine
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-methylphenyl)amine
-
-
-
?
2.5.1.2
thiamine + 4-pyridinecarboxylic acid
i.e. gamma-picolinic acid
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-carboxypyridinium
-
-
-
?
2.5.1.2
thiamine + 6-aminohexanoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 6-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]hexanoic acid
-
-
-
?
2.5.1.2
thiamine + adenine
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + alpha-picoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]alpha-picolinium
-
-
-
?
2.5.1.2
thiamine + aniline
-
636851
Marsilea drummondii
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Marsilea drummondii
?
2.5.1.2
thiamine + aniline
-
636851
Pteridium esculentum
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Pteridium esculentum
?
2.5.1.2
thiamine + aniline
-
636851
Cheilanthes sieberi
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Cheilanthes sieberi
?
2.5.1.2
thiamine + aniline
-
636851
Velesunio ambiguus
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Velesunio ambiguus
?
2.5.1.2
thiamine + beta-picoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]beta-picolinium
-
-
-
?
2.5.1.2
thiamine + gamma-picoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]gamma-picolinium
-
-
-
?
2.5.1.2
thiamine + hydroxyproline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
-
?
2.5.1.2
thiamine + hydroxyproline
-
636851
Pteridium esculentum
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
-
?
2.5.1.2
thiamine + imidazole
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine
-
-
-
?
2.5.1.2
thiamine + L-cysteine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid
-
-
-
?
2.5.1.2
thiamine + m-aminobenzoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 3-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
-
-
?
2.5.1.2
thiamine + m-phenylene diamine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-aminophenyl)amine
-
-
-
?
2.5.1.2
thiamine + methylamine
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + nicotinic acid
-
636851
Pteridium esculentum
?
-
-
-
?
2.5.1.2
thiamine + nicotinic acid
-
636851
Cheilanthes sieberi
?
-
-
-
?
2.5.1.2
thiamine + nicotinic acid
-
636851
Velesunio ambiguus
?
-
-
-
?
2.5.1.2
thiamine + o-aminobenzoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
-
-
?
2.5.1.2
thiamine + o-phenylene diamine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-aminophenyl)amine
-
-
-
?
2.5.1.2
thiamine + p-aminobenzoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 4-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
-
-
?
2.5.1.2
thiamine + p-phenylene diamine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-aminophenyl)amine
-
-
-
?
2.5.1.2
thiamine + proline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
-
?
2.5.1.2
thiamine + proline
-
636851
Pteridium esculentum
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
-
?
2.5.1.2
thiamine + pyridine
-
636851
Marsilea drummondii
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Marsilea drummondii
?
2.5.1.2
thiamine + pyridine
-
636851
Pteridium esculentum
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Pteridium esculentum
?
2.5.1.2
thiamine + pyridine
-
636851
Cheilanthes sieberi
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Cheilanthes sieberi
?
2.5.1.2
thiamine + pyridine
-
636851
Velesunio ambiguus
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Velesunio ambiguus
?
2.5.1.2
thiamine + quinoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium
-
-
-
?
2.5.1.2
thiamine + sulfanilamide
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + sulfanilic acid
-
636851
Marsilea drummondii
?
-
-
-
?
2.5.1.2
thiamine + Tris
-
636851
Marsilea drummondii
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.2
8
9
-
Marsilea drummondii
2.5.1.2
8
9
-
Pteridium esculentum
2.5.1.2
8
9
-
Cheilanthes sieberi
2.5.1.2
8
9
-
Velesunio ambiguus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.5.1.2
3
12
-
Marsilea drummondii
2.5.1.2
3
12
-
Pteridium esculentum
2.5.1.2
3
12
-
Cheilanthes sieberi
2.5.1.2
3
12
-
Velesunio ambiguus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.5.1.2
3
12
stable in this range
Marsilea drummondii
2.5.1.2
3
12
stable in this range
Pteridium esculentum
2.5.1.2
3
12
stable in this range
Cheilanthes sieberi
2.5.1.2
3
12
stable in this range
Velesunio ambiguus