The purification and properties of a thiaminase I enzyme from nardoo (Marsilea Drumondii)

Data extracted from this reference:

EC Number

Activating Compound

Commentary

Organism

Structure

2.5.1.2

2-mercaptoethanol

complete reactivation at 3.3 mM but inhibition at 33 mM

Marsilea drummondii

2.5.1.2

cysteine

complete reactivation at 3.3 mM but inhibition at 33 mM

Marsilea drummondii

2.5.1.2

Thioglycollate

greater reactivation at 33 mM than at 3.3 mM

Marsilea drummondii

2.5.1.2

thiosulphate

greater reactivation at 33 mM than at 3.3 mM

Marsilea drummondii

EC Number

Inhibitors

Commentary

Organism

Structure

2.5.1.2

Ag+

100% inhibition at 1 mM

Marsilea drummondii

2.5.1.2

Cu2+

50% of inhibition at 1 mM

Marsilea drummondii

2.5.1.2

Fe2+

70% of inhibition at 1 mM

Marsilea drummondii

2.5.1.2

HgCl2

potent inhibitor at 0.001 mM

Cheilanthes sieberi

2.5.1.2

HgCl2

potent inhibitor at 0.001 mM

Marsilea drummondii

2.5.1.2

HgCl2

potent inhibitor at 0.001 mM

Pteridium esculentum

2.5.1.2

HgCl2

potent inhibitor at 0.001 mM

Velesunio ambiguus

2.5.1.2

Mersalyl acid

potent inhibitor at 0.001 mM

Cheilanthes sieberi

2.5.1.2

Mersalyl acid

potent inhibitor at 0.001 mM

Marsilea drummondii

2.5.1.2

Mersalyl acid

potent inhibitor at 0.001 mM

Pteridium esculentum

2.5.1.2

Mersalyl acid

potent inhibitor at 0.001 mM

Velesunio ambiguus

2.5.1.2

Mn2+

no inhibition

Marsilea drummondii

2.5.1.2

additional information

not inhibited by EDTA

Marsilea drummondii

2.5.1.2

p-chloromercuribenzoate

complete inhibition at 0.001 mM

Cheilanthes sieberi

2.5.1.2

p-chloromercuribenzoate

complete inhibition at 0.001 mM

Marsilea drummondii

2.5.1.2

p-chloromercuribenzoate

complete inhibition at 0.001 mM

Pteridium esculentum

2.5.1.2

p-chloromercuribenzoate

complete inhibition at 0.001 mM

Velesunio ambiguus

EC Number

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

2.5.1.2

0.003

-

thiamine

measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate

Marsilea drummondii

2.5.1.2

0.003

-

thiamine

measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate

Pteridium esculentum

2.5.1.2

0.003

-

thiamine

measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate

Cheilanthes sieberi

2.5.1.2

0.003

-

thiamine

measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate

Velesunio ambiguus

2.5.1.2

0.5

-

beta-picoline

-

Marsilea drummondii

2.5.1.2

0.7

-

Pyridine

-

Pteridium esculentum

2.5.1.2

0.7

-

Pyridine

-

Velesunio ambiguus

2.5.1.2

0.9

-

nicotinic acid

-

Velesunio ambiguus

2.5.1.2

0.9

-

gamma-Picoline

-

Marsilea drummondii

2.5.1.2

1.4

-

aniline

lower maximal activity than pyridine and nicotinic acid

Marsilea drummondii

2.5.1.2

1.4

-

aniline

lower maximal activity than pyridine and nicotinic acid

Pteridium esculentum

2.5.1.2

1.7

-

m-phenylene diamine

-

Marsilea drummondii

2.5.1.2

1.7

-

o-phenylene diamine

-

Marsilea drummondii

2.5.1.2

2

-

aniline

lower maximal activity than pyridine and nicotinic acid

Velesunio ambiguus

2.5.1.2

2.4

-

m-Toluidine

-

Marsilea drummondii

2.5.1.2

2.5

-

Pyridine

-

Marsilea drummondii

2.5.1.2

2.8

-

3-hydroxyaniline

-

Marsilea drummondii

2.5.1.2

2.9

-

3-aminopyridine

-

Marsilea drummondii

2.5.1.2

3.3

-

Quinoline

-

Marsilea drummondii

2.5.1.2

3.3

-

alpha-picoline

-

Marsilea drummondii

2.5.1.2

3.3

-

p-phenylene diamine

-

Marsilea drummondii

2.5.1.2

3.4

-

gamma-picolinic acid

-

Marsilea drummondii

2.5.1.2

4.2

-

2-hydroxyaniline

-

Marsilea drummondii

2.5.1.2

5

-

Pyridine

-

Cheilanthes sieberi

2.5.1.2

5

-

o-mercaptobenzoic acid

best cosubstrate

Marsilea drummondii

2.5.1.2

5.1

-

aniline

lower maximal activity than pyridine and nicotinic acid

Cheilanthes sieberi

2.5.1.2

6.4

-

o-toluidine

-

Marsilea drummondii

2.5.1.2

9.5

-

adenine

-

Marsilea drummondii

2.5.1.2

12.5

-

2-aminopyridine

-

Marsilea drummondii

2.5.1.2

15.4

-

4-aminopyridine

-

Marsilea drummondii

2.5.1.2

20

-

4-Hydroxyaniline

-

Marsilea drummondii

2.5.1.2

21.2

-

alpha-picolinic acid

-

Marsilea drummondii

2.5.1.2

25

-

nicotinic acid

-

Marsilea drummondii

2.5.1.2

25

-

beta-picolinic acid

-

Marsilea drummondii

2.5.1.2

29

-

nicotinic acid

-

Pteridium esculentum

2.5.1.2

32

-

methylamine

-

Marsilea drummondii

2.5.1.2

33

-

L-cysteine

-

Marsilea drummondii

2.5.1.2

37

-

o-aminobenzoic acid

-

Marsilea drummondii

2.5.1.2

37

-

m-aminobenzoic acid

-

Marsilea drummondii

2.5.1.2

38

-

6-aminohexanoic acid

-

Marsilea drummondii

2.5.1.2

50

-

Sulfanilic acid

-

Marsilea drummondii

2.5.1.2

50

-

3-nitroaniline

-

Marsilea drummondii

2.5.1.2

55

-

nicotinic acid

-

Cheilanthes sieberi

2.5.1.2

77

-

sulfanilamide

-

Marsilea drummondii

2.5.1.2

111

-

proline

-

Marsilea drummondii

2.5.1.2

125

-

imidazole

-

Marsilea drummondii

2.5.1.2

200

-

Tris

-

Marsilea drummondii

2.5.1.2

200

-

p-Aminobenzoic acid

-

Marsilea drummondii

2.5.1.2

400

-

hydroxyproline

-

Marsilea drummondii

EC Number

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2.5.1.2

thiamine + adenine

Marsilea drummondii

-

?

-

-

?

2.5.1.2

thiamine + hydroxyproline

Marsilea drummondii

-

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid

-

-

?

2.5.1.2

thiamine + hydroxyproline

Pteridium esculentum

-

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid

-

-

?

2.5.1.2

thiamine + proline

Marsilea drummondii

-

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid

-

-

?

2.5.1.2

thiamine + proline

Pteridium esculentum

-

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid

-

-

?

EC Number

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2.5.1.2

thiamine + 2-aminopyridine

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + 2-hydroxyaniline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-hydroxyphenyl)amine

-

-

-

?

2.5.1.2

thiamine + 2-mercaptobenzoic acid

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + 2-methylbenzeneamine

i.e. o-toluidine

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-methylphenyl)amine

-

-

-

?

2.5.1.2

thiamine + 2-pyridinecarboxylic acid

i.e. alpha-picolinic acid

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxypyridinium

-

-

-

?

2.5.1.2

thiamine + 3-aminopyridine

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + 3-hydroxyaniline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-hydroxyphenyl)amine

-

-

-

?

2.5.1.2

thiamine + 3-methylbenzeneamine

i.e. m-toluidine

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-methylphenyl)amine

-

-

-

?

2.5.1.2

thiamine + 3-nitroaniline

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + 3-pyridinecarboxylic acid

i.e. beta-picolinic acid

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-carboxypyridinium

-

-

-

?

2.5.1.2

thiamine + 4-aminopyridine

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + 4-hydroxyaniline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-hydroxyphenyl)amine

-

-

-

?

2.5.1.2

thiamine + 4-methylbenzeneamine

i.e. p-toluidine

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-methylphenyl)amine

-

-

-

?

2.5.1.2

thiamine + 4-pyridinecarboxylic acid

i.e. gamma-picolinic acid

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-carboxypyridinium

-

-

-

?

2.5.1.2

thiamine + 6-aminohexanoic acid

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 6-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]hexanoic acid

-

-

-

?

2.5.1.2

thiamine + adenine

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + alpha-picoline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]alpha-picolinium

-

-

-

?

2.5.1.2

thiamine + aniline

-

636851

Marsilea drummondii

5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Marsilea drummondii

?

2.5.1.2

thiamine + aniline

-

636851

Pteridium esculentum

5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Pteridium esculentum

?

2.5.1.2

thiamine + aniline

-

636851

Cheilanthes sieberi

5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Cheilanthes sieberi

?

2.5.1.2

thiamine + aniline

-

636851

Velesunio ambiguus

5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Velesunio ambiguus

?

2.5.1.2

thiamine + beta-picoline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]beta-picolinium

-

-

-

?

2.5.1.2

thiamine + gamma-picoline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]gamma-picolinium

-

-

-

?

2.5.1.2

thiamine + hydroxyproline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid

-

-

-

?

2.5.1.2

thiamine + hydroxyproline

-

636851

Pteridium esculentum

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid

-

-

-

?

2.5.1.2

thiamine + imidazole

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine

-

-

-

?

2.5.1.2

thiamine + L-cysteine

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid

-

-

-

?

2.5.1.2

thiamine + m-aminobenzoic acid

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 3-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid

-

-

-

?

2.5.1.2

thiamine + m-phenylene diamine

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-aminophenyl)amine

-

-

-

?

2.5.1.2

thiamine + methylamine

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + nicotinic acid

-

636851

Pteridium esculentum

?

-

-

-

?

2.5.1.2

thiamine + nicotinic acid

-

636851

Cheilanthes sieberi

?

-

-

-

?

2.5.1.2

thiamine + nicotinic acid

-

636851

Velesunio ambiguus

?

-

-

-

?

2.5.1.2

thiamine + o-aminobenzoic acid

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid

-

-

-

?

2.5.1.2

thiamine + o-phenylene diamine

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-aminophenyl)amine

-

-

-

?

2.5.1.2

thiamine + p-aminobenzoic acid

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 4-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid

-

-

-

?

2.5.1.2

thiamine + p-phenylene diamine

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-aminophenyl)amine

-

-

-

?

2.5.1.2

thiamine + proline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid

-

-

-

?

2.5.1.2

thiamine + proline

-

636851

Pteridium esculentum

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid

-

-

-

?

2.5.1.2

thiamine + pyridine

-

636851

Marsilea drummondii

heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Marsilea drummondii

?

2.5.1.2

thiamine + pyridine

-

636851

Pteridium esculentum

heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Pteridium esculentum

?

2.5.1.2

thiamine + pyridine

-

636851

Cheilanthes sieberi

heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Cheilanthes sieberi

?

2.5.1.2

thiamine + pyridine

-

636851

Velesunio ambiguus

heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Velesunio ambiguus

?

2.5.1.2

thiamine + quinoline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium

-

-

-

?

2.5.1.2

thiamine + sulfanilamide

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + sulfanilic acid

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + Tris

-

636851

Marsilea drummondii

?

-

-

-

?

EC Number

Activating Compound

Commentary

Organism

Structure

2.5.1.2

2-mercaptoethanol

complete reactivation at 3.3 mM but inhibition at 33 mM

Marsilea drummondii

2.5.1.2

cysteine

complete reactivation at 3.3 mM but inhibition at 33 mM

Marsilea drummondii

2.5.1.2

Thioglycollate

greater reactivation at 33 mM than at 3.3 mM

Marsilea drummondii

2.5.1.2

thiosulphate

greater reactivation at 33 mM than at 3.3 mM

Marsilea drummondii

EC Number

Inhibitors

Commentary

Organism

Structure

2.5.1.2

Ag+

100% inhibition at 1 mM

Marsilea drummondii

2.5.1.2

Cu2+

50% of inhibition at 1 mM

Marsilea drummondii

2.5.1.2

Fe2+

70% of inhibition at 1 mM

Marsilea drummondii

2.5.1.2

HgCl2

potent inhibitor at 0.001 mM

Marsilea drummondii

2.5.1.2

HgCl2

potent inhibitor at 0.001 mM

Pteridium esculentum

2.5.1.2

HgCl2

potent inhibitor at 0.001 mM

Cheilanthes sieberi

2.5.1.2

HgCl2

potent inhibitor at 0.001 mM

Velesunio ambiguus

2.5.1.2

Mersalyl acid

potent inhibitor at 0.001 mM

Marsilea drummondii

2.5.1.2

Mersalyl acid

potent inhibitor at 0.001 mM

Pteridium esculentum

2.5.1.2

Mersalyl acid

potent inhibitor at 0.001 mM

Cheilanthes sieberi

2.5.1.2

Mersalyl acid

potent inhibitor at 0.001 mM

Velesunio ambiguus

2.5.1.2

Mn2+

no inhibition

Marsilea drummondii

2.5.1.2

additional information

not inhibited by EDTA

Marsilea drummondii

2.5.1.2

p-chloromercuribenzoate

complete inhibition at 0.001 mM

Marsilea drummondii

2.5.1.2

p-chloromercuribenzoate

complete inhibition at 0.001 mM

Pteridium esculentum

2.5.1.2

p-chloromercuribenzoate

complete inhibition at 0.001 mM

Cheilanthes sieberi

2.5.1.2

p-chloromercuribenzoate

complete inhibition at 0.001 mM

Velesunio ambiguus

EC Number

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

2.5.1.2

0.003

-

thiamine

measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate

Marsilea drummondii

2.5.1.2

0.003

-

thiamine

measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate

Pteridium esculentum

2.5.1.2

0.003

-

thiamine

measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate

Cheilanthes sieberi

2.5.1.2

0.003

-

thiamine

measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate

Velesunio ambiguus

2.5.1.2

0.5

-

beta-picoline

-

Marsilea drummondii

2.5.1.2

0.7

-

Pyridine

-

Pteridium esculentum

2.5.1.2

0.7

-

Pyridine

-

Velesunio ambiguus

2.5.1.2

0.9

-

nicotinic acid

-

Velesunio ambiguus

2.5.1.2

0.9

-

gamma-Picoline

-

Marsilea drummondii

2.5.1.2

1.4

-

aniline

lower maximal activity than pyridine and nicotinic acid

Marsilea drummondii

2.5.1.2

1.4

-

aniline

lower maximal activity than pyridine and nicotinic acid

Pteridium esculentum

2.5.1.2

1.7

-

m-phenylene diamine

-

Marsilea drummondii

2.5.1.2

1.7

-

o-phenylene diamine

-

Marsilea drummondii

2.5.1.2

2

-

aniline

lower maximal activity than pyridine and nicotinic acid

Velesunio ambiguus

2.5.1.2

2.4

-

m-Toluidine

-

Marsilea drummondii

2.5.1.2

2.5

-

Pyridine

-

Marsilea drummondii

2.5.1.2

2.8

-

3-hydroxyaniline

-

Marsilea drummondii

2.5.1.2

2.9

-

3-aminopyridine

-

Marsilea drummondii

2.5.1.2

3.3

-

Quinoline

-

Marsilea drummondii

2.5.1.2

3.3

-

alpha-picoline

-

Marsilea drummondii

2.5.1.2

3.3

-

p-phenylene diamine

-

Marsilea drummondii

2.5.1.2

3.4

-

gamma-picolinic acid

-

Marsilea drummondii

2.5.1.2

4.2

-

2-hydroxyaniline

-

Marsilea drummondii

2.5.1.2

5

-

Pyridine

-

Cheilanthes sieberi

2.5.1.2

5

-

o-mercaptobenzoic acid

best cosubstrate

Marsilea drummondii

2.5.1.2

5.1

-

aniline

lower maximal activity than pyridine and nicotinic acid

Cheilanthes sieberi

2.5.1.2

6.4

-

o-toluidine

-

Marsilea drummondii

2.5.1.2

9.5

-

adenine

-

Marsilea drummondii

2.5.1.2

12.5

-

2-aminopyridine

-

Marsilea drummondii

2.5.1.2

15.4

-

4-aminopyridine

-

Marsilea drummondii

2.5.1.2

20

-

4-Hydroxyaniline

-

Marsilea drummondii

2.5.1.2

21.2

-

alpha-picolinic acid

-

Marsilea drummondii

2.5.1.2

25

-

nicotinic acid

-

Marsilea drummondii

2.5.1.2

25

-

beta-picolinic acid

-

Marsilea drummondii

2.5.1.2

29

-

nicotinic acid

-

Pteridium esculentum

2.5.1.2

32

-

methylamine

-

Marsilea drummondii

2.5.1.2

33

-

L-cysteine

-

Marsilea drummondii

2.5.1.2

37

-

o-aminobenzoic acid

-

Marsilea drummondii

2.5.1.2

37

-

m-aminobenzoic acid

-

Marsilea drummondii

2.5.1.2

38

-

6-aminohexanoic acid

-

Marsilea drummondii

2.5.1.2

50

-

Sulfanilic acid

-

Marsilea drummondii

2.5.1.2

50

-

3-nitroaniline

-

Marsilea drummondii

2.5.1.2

55

-

nicotinic acid

-

Cheilanthes sieberi

2.5.1.2

77

-

sulfanilamide

-

Marsilea drummondii

2.5.1.2

111

-

proline

-

Marsilea drummondii

2.5.1.2

125

-

imidazole

-

Marsilea drummondii

2.5.1.2

200

-

Tris

-

Marsilea drummondii

2.5.1.2

200

-

p-Aminobenzoic acid

-

Marsilea drummondii

2.5.1.2

400

-

hydroxyproline

-

Marsilea drummondii

EC Number

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2.5.1.2

thiamine + adenine

Marsilea drummondii

-

?

-

-

?

2.5.1.2

thiamine + hydroxyproline

Marsilea drummondii

-

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid

-

-

?

2.5.1.2

thiamine + hydroxyproline

Pteridium esculentum

-

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid

-

-

?

2.5.1.2

thiamine + proline

Marsilea drummondii

-

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid

-

-

?

2.5.1.2

thiamine + proline

Pteridium esculentum

-

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid

-

-

?

EC Number

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2.5.1.2

thiamine + 2-aminopyridine

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + 2-hydroxyaniline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-hydroxyphenyl)amine

-

-

-

?

2.5.1.2

thiamine + 2-mercaptobenzoic acid

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + 2-methylbenzeneamine

i.e. o-toluidine

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-methylphenyl)amine

-

-

-

?

2.5.1.2

thiamine + 2-pyridinecarboxylic acid

i.e. alpha-picolinic acid

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxypyridinium

-

-

-

?

2.5.1.2

thiamine + 3-aminopyridine

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + 3-hydroxyaniline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-hydroxyphenyl)amine

-

-

-

?

2.5.1.2

thiamine + 3-methylbenzeneamine

i.e. m-toluidine

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-methylphenyl)amine

-

-

-

?

2.5.1.2

thiamine + 3-nitroaniline

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + 3-pyridinecarboxylic acid

i.e. beta-picolinic acid

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-carboxypyridinium

-

-

-

?

2.5.1.2

thiamine + 4-aminopyridine

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + 4-hydroxyaniline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-hydroxyphenyl)amine

-

-

-

?

2.5.1.2

thiamine + 4-methylbenzeneamine

i.e. p-toluidine

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-methylphenyl)amine

-

-

-

?

2.5.1.2

thiamine + 4-pyridinecarboxylic acid

i.e. gamma-picolinic acid

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-carboxypyridinium

-

-

-

?

2.5.1.2

thiamine + 6-aminohexanoic acid

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 6-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]hexanoic acid

-

-

-

?

2.5.1.2

thiamine + adenine

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + alpha-picoline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]alpha-picolinium

-

-

-

?

2.5.1.2

thiamine + aniline

-

636851

Marsilea drummondii

5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Marsilea drummondii

?

2.5.1.2

thiamine + aniline

-

636851

Pteridium esculentum

5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Pteridium esculentum

?

2.5.1.2

thiamine + aniline

-

636851

Cheilanthes sieberi

5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Cheilanthes sieberi

?

2.5.1.2

thiamine + aniline

-

636851

Velesunio ambiguus

5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Velesunio ambiguus

?

2.5.1.2

thiamine + beta-picoline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]beta-picolinium

-

-

-

?

2.5.1.2

thiamine + gamma-picoline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]gamma-picolinium

-

-

-

?

2.5.1.2

thiamine + hydroxyproline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid

-

-

-

?

2.5.1.2

thiamine + hydroxyproline

-

636851

Pteridium esculentum

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid

-

-

-

?

2.5.1.2

thiamine + imidazole

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine

-

-

-

?

2.5.1.2

thiamine + L-cysteine

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid

-

-

-

?

2.5.1.2

thiamine + m-aminobenzoic acid

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 3-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid

-

-

-

?

2.5.1.2

thiamine + m-phenylene diamine

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-aminophenyl)amine

-

-

-

?

2.5.1.2

thiamine + methylamine

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + nicotinic acid

-

636851

Pteridium esculentum

?

-

-

-

?

2.5.1.2

thiamine + nicotinic acid

-

636851

Cheilanthes sieberi

?

-

-

-

?

2.5.1.2

thiamine + nicotinic acid

-

636851

Velesunio ambiguus

?

-

-

-

?

2.5.1.2

thiamine + o-aminobenzoic acid

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid

-

-

-

?

2.5.1.2

thiamine + o-phenylene diamine

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-aminophenyl)amine

-

-

-

?

2.5.1.2

thiamine + p-aminobenzoic acid

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 4-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid

-

-

-

?

2.5.1.2

thiamine + p-phenylene diamine

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-aminophenyl)amine

-

-

-

?

2.5.1.2

thiamine + proline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid

-

-

-

?

2.5.1.2

thiamine + proline

-

636851

Pteridium esculentum

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid

-

-

-

?

2.5.1.2

thiamine + pyridine

-

636851

Marsilea drummondii

heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Marsilea drummondii

?

2.5.1.2

thiamine + pyridine

-

636851

Pteridium esculentum

heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Pteridium esculentum

?

2.5.1.2

thiamine + pyridine

-

636851

Cheilanthes sieberi

heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Cheilanthes sieberi

?

2.5.1.2

thiamine + pyridine

-

636851

Velesunio ambiguus

heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole

-

636851

Velesunio ambiguus

?

2.5.1.2

thiamine + quinoline

-

636851

Marsilea drummondii

4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium

-

-

-

?

2.5.1.2

thiamine + sulfanilamide

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + sulfanilic acid

-

636851

Marsilea drummondii

?

-

-

-

?

2.5.1.2

thiamine + Tris

-

636851

Marsilea drummondii

?

-

-

-

?