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Literature summary extracted from

  • Agee, C.C.; Airth, R.L.
    Reversible inactivation of thiaminase I of Bacillus thiaminolyticus by its primary substrate, thiamine (1973), J. Bacteriol., 115, 957-965.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.2 2-mercaptoethanol reactivates enzyme less effectively than dithiothreitol Paenibacillus thiaminolyticus
2.5.1.2 dithiothreitol reactivates 60-100% of the original activity Paenibacillus thiaminolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.2 additional information acidic conditions prevent inactivation but low pH does not restore activity after inactivation, quaternary nitrogen linked to a substituted pyrimidine ring through a methylene bridge is shared by compounds that inactivates the enzyme, inactivation alters either the net charge or the structure of the protein or both Paenibacillus thiaminolyticus
2.5.1.2 thiamine inactivation is proportional to thiamine concentration and the thiamine/enzyme ratio is 1 mol/1 mol Paenibacillus thiaminolyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.2 extracellular
-
 Paenibacillus thiaminolyticus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.2 Paenibacillus thiaminolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.2 Tris-glycine polyacrylamide electrophoresis Paenibacillus thiaminolyticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.2 cell culture
-
 Paenibacillus thiaminolyticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.2 thiamine + aniline one molecule of thiamine is cleaved releasing one molecule of methylhydroxyethylthiazole for one molecule of enzyme Paenibacillus thiaminolyticus 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.2 25
-
 assay at Paenibacillus thiaminolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.2 5.8
-
 0.1 M sodium phosphate buffer Paenibacillus thiaminolyticus