Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wittliff, J.L.; Airth, R.L.
    The extracellular thiaminase I of Bacillus thiaminolyticus. I. Purification and physicochemical properties (1968), Biochemistry, 7, 736-744.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.2 aniline highly inhibitory above 2.4 mM Paenibacillus thiaminolyticus
2.5.1.2 thiamine slightly inhibitory above 0.035 mM Paenibacillus thiaminolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.2 0.0087
-
 thiamine pH 5.8, 25°C, the enzyme does not follow strict Michaelis-Menten kinetics Paenibacillus thiaminolyticus
2.5.1.2 2.9
-
 aniline pH 5.8, 25°C, the enzyme does not follow strict Michaelis-Menten kinetics Paenibacillus thiaminolyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.2 extracellular
-
 Paenibacillus thiaminolyticus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.2 44000
-
 sedimentation and diffusion data Paenibacillus thiaminolyticus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.2 Paenibacillus thiaminolyticus
-
 M strain
-
2.5.1.2 Paenibacillus thiaminolyticus M
-
 M strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.2 by ammonium precipitation, Sephadex G-25 column, Sephadex G-100 column and DEAE-Sephadex column Paenibacillus thiaminolyticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.2 culture filtrate
-
 Paenibacillus thiaminolyticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.2 thiamine + aniline
-
 Paenibacillus thiaminolyticus 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + aniline
-
 Paenibacillus thiaminolyticus M 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.2 37
-
 in 0.1 M sodium phosphate buffer at pH 5.8 Paenibacillus thiaminolyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.2 60
-
 more than 90% of activity remains after 20 min Paenibacillus thiaminolyticus
2.5.1.2 65
-
 activity abolished after 20 min Paenibacillus thiaminolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.2 5.8 6.8 assay at 25ºC Paenibacillus thiaminolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.2 5.8
-
 0.1 M sodium phosphate buffer Paenibacillus thiaminolyticus