EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.145 | I102M/N117S/M118V/D208N | clones harbouring the mutant enzyme are more sensitive to 3'-azido-2',3'-dideoxythymidine and beta-D-arabinofuranosylcytosine | Drosophila melanogaster |
2.7.1.145 | I47T/N210D | clones harbouring the mutant enzyme are more sensitive to 3'-azido-2',3'-dideoxythymidine and beta-D-arabinofuranosylcytosine | Drosophila melanogaster |
2.7.1.145 | M1T/T85A/N121S | clones harbouring the mutant enzyme are more sensitive to 1-beta-D-arabinofuranosylcytosine and 2',3'-dideoxycytidine | Drosophila melanogaster |
2.7.1.145 | N210D/L239P | clones harbouring the mutant enzyme are more sensitive to 3'-azido-2',3'-dideoxythymidine and beta-D-arabinofuranosylcytosine | Drosophila melanogaster |
2.7.1.145 | N38D/N64D | clones harbouring the mutant enzyme are more sensitive specifically to 3'-azido-2',3'-dideoxythymidine | Drosophila melanogaster |
2.7.1.145 | N45D/N64D | clones harbouring the mutant enzyme are more sensitive to 3'-azido-2',3'-dideoxythymidine and other nucleoside analogs | Drosophila melanogaster |
2.7.1.145 | N45D/N64D | mutant enzyme with the largest und universal increase in sensitivity of all mutants tested. 316fold increase to 3'-azido-2',3'-dideoxythymidine, more than 11fold to 2',3'-dideoxycytidine, and 3.2fold increase in sensitivity to beta-D-arabinofuranosylcytosine and 2',3'-dideoxyadenosine. Higher Km-values for native substrates than wild-type enzyme and Vmax-values are substantially lower, decrease in feedback inhibition by TTP, Km and Vmax values for 3'-azido-2',3'-dideoxythymidine and Km value for 2',3'-dideoxycytidine are nearly unchanged | Drosophila melanogaster |
2.7.1.145 | N59D/M118V/Y179H | clones harbouring the mutant enzyme are more sensitive to 3'-azido-2',3'-dideoxythymidine and beta-D-arabinofuranosylcytosine | Drosophila melanogaster |
2.7.1.145 | N64S/L68S/M69L | clones harbouring the mutant enzyme are more sensitive specifically to 3'-azido-2',3'-dideoxythymidine | Drosophila melanogaster |
2.7.1.145 | T12A/V84A/N213S | clones harbouring the mutant enzyme are more sensitive to 1-beta-D-arabinofuranosylcytosine | Drosophila melanogaster |
2.7.1.145 | T85A | clones harbouring the mutant enzyme are more sensitive to 1-beta-D-arabinofuranosylcytosine and 2',3'-dideoxycytidine | Drosophila melanogaster |
2.7.1.145 | V84A | clones harbouring the mutant enzyme are more sensitive to 1-beta-D-arabinofuranosylcytosine | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.145 | TTP | mutant enzyme N45D/N64D shows a decrease in feedback inhibition compared to the wild-type enzyme | Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.145 | 0.0072 | - |
3'-azido-2',3'-dideoxythymidine | mutant enzyme N45D/N64D | Drosophila melanogaster | |
2.7.1.145 | 0.0083 | - |
3'-azido-2',3'-dideoxythymidine | - |
Drosophila melanogaster | |
2.7.1.145 | 0.0242 | - |
thymidine | mutant enzyme N45D/N64D | Drosophila melanogaster | |
2.7.1.145 | 0.0964 | - |
2'-deoxycytidine | mutant enzyme N45D/N64D | Drosophila melanogaster | |
2.7.1.145 | 1.093 | - |
2',3'-dideoxycytidine | mutant enzyme N45D/N64D | Drosophila melanogaster | |
2.7.1.145 | 1.124 | - |
2',3'-dideoxycytidine | - |
Drosophila melanogaster | |
2.7.1.145 | 2.004 | - |
2'-deoxyguanosine | mutant enzyme N45D/N64D | Drosophila melanogaster | |
2.7.1.145 | 3.16 | - |
2'-deoxyadenosine | mutant enzyme N45D/N64D | Drosophila melanogaster |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.145 | Drosophila melanogaster | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.145 | (E)-5-(2-bromovinyl)-2'-deoxyuridine + ATP | 54% of the activity with thymidine | Drosophila melanogaster | (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | 1-beta-D-arabinofuranosylcytosine + ATP | 58.2% of the activity with thymidine | Drosophila melanogaster | 1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | 1-beta-D-arabinofuranosylthymine + ATP | 53.9% of the activity with thymidine | Drosophila melanogaster | 1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | 2',3'-dideoxyadenosine + ATP | 0.9% of the activity with thymidine | Drosophila melanogaster | 2',3'-dideoxyadenosine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | 2',3'-dideoxycytidine + ATP | - |
Drosophila melanogaster | 2',3'-dideoxycytidine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | 2'-deoxyadenosine + ATP | - |
Drosophila melanogaster | 2'-deoxyadenosine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | 2'-deoxycytidine + ATP | - |
Drosophila melanogaster | 2'-deoxycytidine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | 2'-deoxyguanosine + ATP | - |
Drosophila melanogaster | 2'-deoxyguanosine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | 2'-deoxyuridine + ATP | 113% of the activity with thymidine | Drosophila melanogaster | 2'-deoxyuridine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | 2-chloro-2'-deoxyadenosine + ATP | 126% of the activity with thymidine | Drosophila melanogaster | 2-chloro-2'-deoxyadenosine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | 3'-azido-2',3'-dideoxythymidine + ATP | - |
Drosophila melanogaster | 3'-azido-2',3'-dideoxythymidine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | 5-fluoro-2'-deoxyuridine + ATP | 92% of the activity with thymidine | Drosophila melanogaster | 5-fluoro-2'-deoxyuridine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | adenosine + ATP | 0.82% of the activity with thymidine | Drosophila melanogaster | adenosine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | cytidine + ATP | 16% of the activity with thymidine | Drosophila melanogaster | cytidine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | guanosine + ATP | 1.18% of the activity with thymidine | Drosophila melanogaster | guanosine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | thymidine + ATP | - |
Drosophila melanogaster | thymidine 5'-phosphate + ADP | - |
? | |
2.7.1.145 | uridine + ATP | 6.45% of the activity with thymidine | Drosophila melanogaster | uridine 5'-phosphate + ADP | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.145 | 0.036 | - |
3'-azido-2',3'-dideoxythymidine | - |
Drosophila melanogaster | |
2.7.1.145 | 0.052 | - |
3'-azido-2',3'-dideoxythymidine | mutant enzyme N45D/N64D | Drosophila melanogaster | |
2.7.1.145 | 0.076 | - |
2'-deoxyguanosine | mutant enzyme N45D/N64D | Drosophila melanogaster | |
2.7.1.145 | 0.54 | - |
2',3'-dideoxycytidine | mutant enzyme N45D/N64D | Drosophila melanogaster | |
2.7.1.145 | 0.828 | - |
2'-deoxyadenosine | mutant enzyme N45D/N64D | Drosophila melanogaster | |
2.7.1.145 | 1.22 | - |
thymidine | mutant enzyme N45D/N64D | Drosophila melanogaster | |
2.7.1.145 | 4.03 | - |
2'-deoxycytidine | mutant enzyme N45D/N64D | Drosophila melanogaster | |
2.7.1.145 | 4.2 | - |
2',3'-dideoxycytidine | - |
Drosophila melanogaster | |
2.7.1.145 | 14.2 | - |
thymidine | pH 7.8, 22°C, recombinant enzyme | Drosophila melanogaster | |
2.7.1.145 | 15.1 | - |
2'-deoxyguanosine | pH 7.8, 22°C, recombinant enzyme | Drosophila melanogaster | |
2.7.1.145 | 16.5 | - |
2'-deoxycytidine | pH 7.8, 22°C, recombinant enzyme | Drosophila melanogaster | |
2.7.1.145 | 20.6 | - |
2'-deoxyadenosine | pH 7.8, 22°C, recombinant enzyme | Drosophila melanogaster |