BRENDA - Enzyme Database show

Cloning and characterization of the multisubstrate deoxyribonucleoside kinase of Drosophila melanogaster

Johansson, M.; van Rompay, A.R.; Degreve, B.; Balzarini, J.; Karlsson, A.; J. Biol. Chem. 274, 23814-23819 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.145
expression in Escherichia coli
Drosophila melanogaster
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.145
(E)-5-(2-bromovinyl)-1-beta-D-arabinofuranosyluracil
IC50: 0.0277 mM for reaction with thymidine and 0.0158 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
(E)-5-(2-bromovinyl)-2'-deoxyuridine
IC50: 0.00257 mM for reaction with thymidine and 0.00194 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil
IC50: 0.0163 mM for reaction with thymidine and 0.0154 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
1-beta-D-arabinofuranosylcytosine
IC50: 0.0534 mM for reaction with thymidine and 0.089 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
1-beta-D-arabinofuranosylthymidine
IC50: 0.0648 mM for reaction with thymidine and 0.0348 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
1-beta-D-arabinofuranosyluracil
IC50: 0.057 mM for reaction with thymidine and 0.058 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
2',2'-difluorodeoxycytidine
IC50: 0.145 mM for reaction with thymidine and 0.102 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
2',3'-dehydro-3'-deoxythymidine
IC50 for reaction with thymidine is greater than 1000 mM, IC50 for reaction with 2'-deoxycytidine is 0.805 mM
Drosophila melanogaster
2.7.1.145
2',3'-dideoxycytidine
IC50: 0.76 mM for reaction with thymidine and 0.838 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
2',3'-dideoxythymidine
IC50: 0.075 mM for reaction with thymidine and 0.0703 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
2'-deoxy-3'-thiacytidine
IC50: 0.74 mM for reaction with thymidine and 0.868 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
2'-deoxycytidine
competitive with thymidine
Drosophila melanogaster
2.7.1.145
2'-deoxyuridine
reaction with thymidine and 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
2-chloro-2'-deoxyadenosine
IC50: 0.123 mM for reaction with thymidine and 0.12 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
3'-azido-2',3'-dideoxythymidine
IC50: 0.0419 mM for reaction with thymidine and 0.0296 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
5-fluoro-2'-deoxyuridine
IC50: 0.0258 mM for reaction with thymidine and 0.02235 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
thymidine
competitive with 2'-deoxycytidine
Drosophila melanogaster
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.0016
-
thymidine
pH 8, 37°C
Drosophila melanogaster
2.7.1.145
0.0026
-
2'-deoxycytidine
pH 8, 37°C
Drosophila melanogaster
2.7.1.145
0.373
-
2'-deoxyadenosine
pH 8, 37°C
Drosophila melanogaster
2.7.1.145
2
-
2'-deoxyguanosine
pH 8, 37°C
Drosophila melanogaster
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.145
29000
-
1 * 29000, calculation from nucleotide sequence
Drosophila melanogaster
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.145
Drosophila melanogaster
Q9XZT6
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
2'-deoxycytidine + ATP
-
636841
Drosophila melanogaster
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
additional information
the recombinant enzyme preferentially phosphorylates the pyrimidine nucleosides, but phosphorylation of the purine nucleosidesis also efficiently catalyzed
636841
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
no activity with TMP
636841
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
thymidine + ATP
-
636841
Drosophila melanogaster
thymidine 5'-phosphate + ADP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.145
monomer
1 * 29000, calculation from nucleotide sequence
Drosophila melanogaster
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.145
0.00257
-
IC50: 0.00257 mM for reaction with thymidine and 0.00194 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
(E)-5-(2-bromovinyl)-2'-deoxyuridine
2.7.1.145
0.0163
-
IC50: 0.0163 mM for reaction with thymidine and 0.0154 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil
2.7.1.145
0.0258
-
IC50: 0.0258 mM for reaction with thymidine and 0.02235 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
5-fluoro-2'-deoxyuridine
2.7.1.145
0.0277
-
IC50: 0.0277 mM for reaction with thymidine and 0.0158 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
(E)-5-(2-bromovinyl)-1-beta-D-arabinofuranosyluracil
2.7.1.145
0.0419
-
IC50: 0.0419 mM for reaction with thymidine and 0.0296 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
3'-azido-2',3'-dideoxythymidine
2.7.1.145
0.0534
-
IC50: 0.0534 mM for reaction with thymidine and 0.089 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
1-beta-D-arabinofuranosylcytosine
2.7.1.145
0.057
-
IC50: 0.057 mM for reaction with thymidine and 0.058 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
1-beta-D-arabinofuranosyluracil
2.7.1.145
0.0648
-
IC50: 0.0648 mM for reaction with thymidine and 0.0348 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
1-beta-D-arabinofuranosylthymidine
2.7.1.145
0.075
-
IC50: 0.075 mM for reaction with thymidine and 0.0703 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2',3'-dideoxythymidine
2.7.1.145
0.123
-
IC50: 0.123 mM for reaction with thymidine and 0.12 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2-chloro-2'-deoxyadenosine
2.7.1.145
0.145
-
IC50: 0.145 mM for reaction with thymidine and 0.102 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2',2'-difluorodeoxycytidine
2.7.1.145
0.74
-
IC50: 0.74 mM for reaction with thymidine and 0.868 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2'-deoxy-3'-thiacytidine
2.7.1.145
0.76
-
IC50: 0.76 mM for reaction with thymidine and 0.838 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2',3'-dideoxycytidine
2.7.1.145
0.805
-
IC50 for reaction with 2'-deoxycytidine is 0.805 mM
Drosophila melanogaster
2',3'-dehydro-3'-deoxythymidine
2.7.1.145
1
-
IC50 for reaction with thymidine is greater than 1000 microM
Drosophila melanogaster
2',3'-dehydro-3'-deoxythymidine
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.145
expression in Escherichia coli
Drosophila melanogaster
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.145
0.00257
-
IC50: 0.00257 mM for reaction with thymidine and 0.00194 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
(E)-5-(2-bromovinyl)-2'-deoxyuridine
2.7.1.145
0.0163
-
IC50: 0.0163 mM for reaction with thymidine and 0.0154 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil
2.7.1.145
0.0258
-
IC50: 0.0258 mM for reaction with thymidine and 0.02235 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
5-fluoro-2'-deoxyuridine
2.7.1.145
0.0277
-
IC50: 0.0277 mM for reaction with thymidine and 0.0158 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
(E)-5-(2-bromovinyl)-1-beta-D-arabinofuranosyluracil
2.7.1.145
0.0419
-
IC50: 0.0419 mM for reaction with thymidine and 0.0296 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
3'-azido-2',3'-dideoxythymidine
2.7.1.145
0.0534
-
IC50: 0.0534 mM for reaction with thymidine and 0.089 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
1-beta-D-arabinofuranosylcytosine
2.7.1.145
0.057
-
IC50: 0.057 mM for reaction with thymidine and 0.058 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
1-beta-D-arabinofuranosyluracil
2.7.1.145
0.0648
-
IC50: 0.0648 mM for reaction with thymidine and 0.0348 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
1-beta-D-arabinofuranosylthymidine
2.7.1.145
0.075
-
IC50: 0.075 mM for reaction with thymidine and 0.0703 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2',3'-dideoxythymidine
2.7.1.145
0.123
-
IC50: 0.123 mM for reaction with thymidine and 0.12 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2-chloro-2'-deoxyadenosine
2.7.1.145
0.145
-
IC50: 0.145 mM for reaction with thymidine and 0.102 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2',2'-difluorodeoxycytidine
2.7.1.145
0.74
-
IC50: 0.74 mM for reaction with thymidine and 0.868 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2'-deoxy-3'-thiacytidine
2.7.1.145
0.76
-
IC50: 0.76 mM for reaction with thymidine and 0.838 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2',3'-dideoxycytidine
2.7.1.145
0.805
-
IC50 for reaction with 2'-deoxycytidine is 0.805 mM
Drosophila melanogaster
2',3'-dehydro-3'-deoxythymidine
2.7.1.145
1
-
IC50 for reaction with thymidine is greater than 1000 microM
Drosophila melanogaster
2',3'-dehydro-3'-deoxythymidine
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.145
(E)-5-(2-bromovinyl)-1-beta-D-arabinofuranosyluracil
IC50: 0.0277 mM for reaction with thymidine and 0.0158 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
(E)-5-(2-bromovinyl)-2'-deoxyuridine
IC50: 0.00257 mM for reaction with thymidine and 0.00194 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil
IC50: 0.0163 mM for reaction with thymidine and 0.0154 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
1-beta-D-arabinofuranosylcytosine
IC50: 0.0534 mM for reaction with thymidine and 0.089 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
1-beta-D-arabinofuranosylthymidine
IC50: 0.0648 mM for reaction with thymidine and 0.0348 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
1-beta-D-arabinofuranosyluracil
IC50: 0.057 mM for reaction with thymidine and 0.058 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
2',2'-difluorodeoxycytidine
IC50: 0.145 mM for reaction with thymidine and 0.102 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
2',3'-dehydro-3'-deoxythymidine
IC50 for reaction with thymidine is greater than 1000 mM, IC50 for reaction with 2'-deoxycytidine is 0.805 mM
Drosophila melanogaster
2.7.1.145
2',3'-dideoxycytidine
IC50: 0.76 mM for reaction with thymidine and 0.838 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
2',3'-dideoxythymidine
IC50: 0.075 mM for reaction with thymidine and 0.0703 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
2'-deoxy-3'-thiacytidine
IC50: 0.74 mM for reaction with thymidine and 0.868 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
2'-deoxycytidine
competitive with thymidine
Drosophila melanogaster
2.7.1.145
2'-deoxyuridine
reaction with thymidine and 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
2-chloro-2'-deoxyadenosine
IC50: 0.123 mM for reaction with thymidine and 0.12 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
3'-azido-2',3'-dideoxythymidine
IC50: 0.0419 mM for reaction with thymidine and 0.0296 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
5-fluoro-2'-deoxyuridine
IC50: 0.0258 mM for reaction with thymidine and 0.02235 mM for reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
thymidine
competitive with 2'-deoxycytidine
Drosophila melanogaster
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.0016
-
thymidine
pH 8, 37°C
Drosophila melanogaster
2.7.1.145
0.0026
-
2'-deoxycytidine
pH 8, 37°C
Drosophila melanogaster
2.7.1.145
0.373
-
2'-deoxyadenosine
pH 8, 37°C
Drosophila melanogaster
2.7.1.145
2
-
2'-deoxyguanosine
pH 8, 37°C
Drosophila melanogaster
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.145
29000
-
1 * 29000, calculation from nucleotide sequence
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
2'-deoxycytidine + ATP
-
636841
Drosophila melanogaster
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
additional information
the recombinant enzyme preferentially phosphorylates the pyrimidine nucleosides, but phosphorylation of the purine nucleosidesis also efficiently catalyzed
636841
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
no activity with TMP
636841
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
thymidine + ATP
-
636841
Drosophila melanogaster
thymidine 5'-phosphate + ADP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.145
monomer
1 * 29000, calculation from nucleotide sequence
Drosophila melanogaster