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Literature summary extracted from
- The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: Current perspectives and a new unifying nomenclature (2002), Plant Cell Physiol., 43, 1421-1435.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.207 | additional information | - |
Pisum sativum | |
| 2.4.1.207 | additional information | At-XTH22 is strongly induced in response to mechanical perturbations such as wind or touch | Arabidopsis thaliana |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.207 | gene structure, genomic localization and phylogenetic relationship of the XTH gene family | Arabidopsis thaliana |
| 3.2.1.120 | overview: gene family | Arabidopsis thaliana |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 2.4.1.207 | E97Q | At-XTH22: a Glu to Gln substitution, converting the active site sequence DEIDFEFL to DQIDFEFL, abolishes XET activity | Arabidopsis thaliana |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.207 | additional information | - |
additional information | recombinant TCH4 protein, Km: 0.62 mg/ml for nonfucosylated tamarind xyloglucan as donor, 1.8 mg/ml for fucosylated pea xyloglucan | Arabidopsis thaliana | |
| 2.4.1.207 | 0.0003 | - |
xyloglucan acceptor | At-XTH22, high MW xyloglucan | Arabidopsis thaliana |  |
| 2.4.1.207 | 0.019 | - |
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc | - |
Pisum sativum | |
| 2.4.1.207 | 0.033 | - |
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc | - |
Pisum sativum | |
| 2.4.1.207 | 0.05 | - |
Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc | - |
Pisum sativum | |
| 2.4.1.207 | 0.073 | - |
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | recombinant At-XTH22 | Arabidopsis thaliana | |
| 2.4.1.207 | 0.2 | - |
Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc | - |
Pisum sativum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.4.1.207 | apoplast | - |
Vigna angularis | 48046 | - |
| 2.4.1.207 | cell wall | - |
Pisum sativum | 5618 | - |
| 2.4.1.207 | cell wall | - |
Tropaeolum majus | 5618 | - |
| 2.4.1.207 | cell wall | - |
Vigna angularis | 5618 | - |
| 2.4.1.207 | cell wall | bound to | Nicotiana tabacum | 5618 | - |
| 2.4.1.207 | additional information | - |
Pisum sativum | - |
- |
| 2.4.1.207 | additional information | - |
Nicotiana tabacum | - |
- |
| 2.4.1.207 | additional information | expression profiles of members of the XTH gene family, each XTH gene is likely to have a unique fingerprint of expression and regulation | Arabidopsis thaliana | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.207 | 33000 | - |
x * 33000, SDS-PAGE | Vigna angularis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | Pisum sativum | - |
? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | Nicotiana tabacum | - |
? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | Solanum lycopersicum | - |
? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | Vigna angularis | - |
? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | Arabidopsis thaliana | existence of different classes of XET with differing roles in vivo | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | Tropaeolum majus | existence of different classes of XET with differing roles in vivo | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | Arabidopsis thaliana | enzyme is capable of splitting and reconnecting xyloglucan molecules in rapidly growing plant tissues, expression and presumed physiological roles of At-XTH22 and 24 | ? | - |
? | |
| 3.2.1.120 | additional information | Arabidopsis thaliana | main roles of enzyme are wall restructuring and integration/wall assembly | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.207 | Apium sp. | - |
- |
- |
| 2.4.1.207 | Arabidopsis thaliana | - |
ecotype Columbia | - |
| 2.4.1.207 | Arabidopsis thaliana | - |
systematic nomenclature of the XTH gene family with 3 subfamilies | - |
| 2.4.1.207 | Nicotiana tabacum | - |
- |
- |
| 2.4.1.207 | Pisum sativum | - |
- |
- |
| 2.4.1.207 | Solanum lycopersicum | - |
- |
- |
| 2.4.1.207 | Spinacia oleracea | - |
spinach | - |
| 2.4.1.207 | Tropaeolum majus | - |
- |
- |
| 2.4.1.207 | Vigna angularis | - |
azuki bean | - |
| 3.2.1.120 | Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.207 | - |
Vigna angularis |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 2.4.1.207 | 2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc | double-displacement reaction mechanism | Tropaeolum majus | |
| 3.2.1.120 | R1-Glcbeta(1-4)Glcbeta[Xylbeta(1-6)Glcbeta(1-4)](1-4)Glcbeta(1-4)Glcbeta(1-4)-R2 + H2O = R1-Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)-R2 + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose | mechanism | Arabidopsis thaliana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.4.1.207 | epicotyl | - |
Pisum sativum | - |
| 2.4.1.207 | epicotyl | - |
Tropaeolum majus | - |
| 2.4.1.207 | epicotyl | - |
Vigna angularis | - |
| 2.4.1.207 | petiole | young petioles, high activity in thick-walled collenchyma cells | Apium sp. | - |
| 2.4.1.207 | root | - |
Tropaeolum majus | - |
| 2.4.1.207 | root | XET activity in the initiating root hairs | Arabidopsis thaliana | - |
| 2.4.1.207 | root | XET activity in the root cell elongation zone | Nicotiana tabacum | - |
| 2.4.1.207 | root | XET activity in the root cell elongation zone | Arabidopsis thaliana | - |
| 2.4.1.207 | seed | - |
Pisum sativum | - |
| 2.4.1.207 | seed | - |
Tropaeolum majus | - |
| 2.4.1.207 | stem | - |
Pisum sativum | - |
| 2.4.1.207 | stem | - |
Solanum lycopersicum | - |
| 2.4.1.207 | stem | - |
Tropaeolum majus | - |
| 2.4.1.207 | stem | - |
Vigna angularis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.120 | additional information | - |
overview: assay methods | Arabidopsis thaliana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | - |
Spinacia oleracea | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | - |
Nicotiana tabacum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Pisum sativum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Arabidopsis thaliana | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Solanum lycopersicum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Tropaeolum majus | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Vigna angularis | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Apium sp. | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | preference for solanaceous xyloglucan without Fuc over xyloglucan from other sources as donor | Solanum lycopersicum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | donor xyloglucan cleavage sites of XTHs from stems and epicotyls | Vigna angularis | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | donor: Glc8-based XXXGXXXG | Tropaeolum majus | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc | - |
Arabidopsis thaliana | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc | preference of XLLG over XXXG over XXFG over XXG | Pisum sativum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc | XXFG | Spinacia oleracea | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc | XXFG | Pisum sativum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc | - |
Tropaeolum majus | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc | XXG, preference of XLLG over XXXG over XXFG over XXG | Pisum sativum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | reaction results in a hybrid product and a leaving group | Arabidopsis thaliana | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | XLLG-ol | Pisum sativum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | XLLG-ol | Arabidopsis thaliana | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | XLLG-ol | Tropaeolum majus | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc | preference of XLLG over XXXG over XXFG over XXG | Pisum sativum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc | XLLG | Pisum sativum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc | XLLG | Tropaeolum majus | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc | preference of XLLG over XXXG over XXFG over XXG | Pisum sativum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc | XXXG | Pisum sativum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc | XXXG | Tropaeolum majus | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta1-PA | pyridylamino XXXG | Vigna angularis | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | - |
Pisum sativum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | - |
Nicotiana tabacum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | - |
Solanum lycopersicum | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | - |
Vigna angularis | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | existence of different classes of XET with differing roles in vivo | Arabidopsis thaliana | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | existence of different classes of XET with differing roles in vivo | Tropaeolum majus | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + xyloglucan acceptor | enzyme is capable of splitting and reconnecting xyloglucan molecules in rapidly growing plant tissues, expression and presumed physiological roles of At-XTH22 and 24 | Arabidopsis thaliana | ? | - |
? | |
| 2.4.1.207 | Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc | acceptor and donor: Glc8-based XXXGXXXG | Tropaeolum majus | Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc | products: XXXGXXXGXXXG + XXXG | ? | |
| 3.2.1.120 | additional information | main roles of enzyme are wall restructuring and integration/wall assembly | Arabidopsis thaliana | ? | - |
? | |
| 3.2.1.120 | xyloglucan fragment oligosaccharide + H2O | - |
Arabidopsis thaliana | isoprimeverose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.207 | ? | - |
Arabidopsis thaliana |
| 2.4.1.207 | ? | - |
Solanum lycopersicum |
| 2.4.1.207 | ? | - |
Tropaeolum majus |
| 2.4.1.207 | ? | x * 33000, SDS-PAGE | Vigna angularis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.207 | XTH | - |
- |
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