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Literature summary extracted from

  • Bourquin, V.; Nishikubo, N.; Abe, H.; Brumer, H.; Denman, S.; Eklund, M.; Christiernin, M.; Teeri, T.T.; Sundberg, B.; Mellerowicz, E.J.
    Xyloglucan endotransglycosylases have a function during the formation of secondary cell walls of vascular tissues (2002), Plant Cell, 14, 3073-3088.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.207 PttXET16A, a XET isoform in secondary vascular tissues and a member of XET subfamily I, is cloned, sequenced and expressed in Escherichia coli BL21(DE3), structure of the coding sequence Populus tremula x Populus tremuloides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.207 donor xyloglucan + xyloglucan acceptor Populus tremula x Populus tremuloides multifunctional role in cell wall construction, role in restructuring primary cell walls at the time when secondary wall layers are deposited, probably creating and reinforcing the connections between the primary and secondary wall layers ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Populus tremula x Populus tremuloides Q8GZD5 hybrid aspen, poplar, major isoenzyme PttXET16A, a member of XET subfamily I
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Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.207 glycoprotein PttXET16A contains a putative N-glycosylation site Populus tremula x Populus tremuloides
2.4.1.207 proteolytic modification PttXET16A has a putative 22 amino acids signal peptide at the N-terminus Populus tremula x Populus tremuloides

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.207 recombinant His6-tagged PttXET16A, expressed in Escherichia coli BL21(DE3) Populus tremula x Populus tremuloides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 leaf PttXET16A is expressed transiently in developing leaves Populus tremula x Populus tremuloides
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2.4.1.207 additional information detailed localization of XET in poplar stems, PttXET16A expression pattern, expression in secondary vascular tissues Populus tremula x Populus tremuloides
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2.4.1.207 phloem XET activity in xylem and phloem fibers at the stage of secondary wall formation, PttXET16A Populus tremula x Populus tremuloides
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2.4.1.207 root PttXET16A expression in young roots and root tips Populus tremula x Populus tremuloides
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2.4.1.207 stem
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 Populus tremula x Populus tremuloides
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2.4.1.207 xylem XET activity in xylem and phloem fibers at the stage of secondary wall formation, PttXET16A Populus tremula x Populus tremuloides
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 donor xyloglucan + acceptor xyloglucan acceptors: sulforhodamine-labeled xyloglucan-oligosaccharides Populus tremula x Populus tremuloides ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor multifunctional role in cell wall construction, role in restructuring primary cell walls at the time when secondary wall layers are deposited, probably creating and reinforcing the connections between the primary and secondary wall layers Populus tremula x Populus tremuloides ?
-
 ?
2.4.1.207 additional information contains strongly conserved sequence motif of XETs: DEIDFEFLG Populus tremula x Populus tremuloides ?
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 ?
2.4.1.207 additional information PttXET16A has 4 conserved Cys residues Populus tremula x Populus tremuloides ?
-
 ?