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Literature summary extracted from

  • Sulova, Z.; Lednicka, M.; Farkas, V.
    A colorimetric assay for xyloglucan-endotransglycosylase from germinating seeds (1995), Anal. Biochem., 229, 80-85.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.207 xyloglucan-derived oligosaccharide stimulates, highest stimulating effect with the nonasaccharide Glc4Xyl3Gal2, lowest one with the pentasaccharide Glc3Xyl2 Tropaeolum majus

General Stability

EC Number General Stability Organism
2.4.1.207 0.1 mM final concentration spermine stabilizes Tropaeolum majus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.207 donor xyloglucan + xyloglucan acceptor Phaseolus vulgaris one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor Pisum sativum one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor Lens culinaris one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor Tamarindus indica one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor Tropaeolum majus one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Lens culinaris
-
-
-
2.4.1.207 Phaseolus vulgaris
-
-
-
2.4.1.207 Pisum sativum
-
-
-
2.4.1.207 Tamarindus indica
-
-
-
2.4.1.207 Tropaeolum majus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.207
-
 Phaseolus vulgaris
2.4.1.207
-
 Pisum sativum
2.4.1.207
-
 Lens culinaris
2.4.1.207
-
 Tamarindus indica
2.4.1.207
-
 Tropaeolum majus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.207 2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides Phaseolus vulgaris
a
2.4.1.207 2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides Pisum sativum
a
2.4.1.207 2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides Lens culinaris
a
2.4.1.207 2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides Tamarindus indica
a
2.4.1.207 2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides Tropaeolum majus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 cotyledon of germinated seeds Phaseolus vulgaris
-
2.4.1.207 cotyledon of germinated seeds Pisum sativum
-
2.4.1.207 cotyledon of germinated seeds Lens culinaris
-
2.4.1.207 cotyledon of germinated seeds Tamarindus indica
-
2.4.1.207 cotyledon of germinated seeds Tropaeolum majus
-
2.4.1.207 seed cotyledons of germinated seeds Phaseolus vulgaris
-
2.4.1.207 seed cotyledons of germinated seeds Pisum sativum
-
2.4.1.207 seed cotyledons of germinated seeds Lens culinaris
-
2.4.1.207 seed cotyledons of germinated seeds Tamarindus indica
-
2.4.1.207 seed cotyledons of germinated seeds Tropaeolum majus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.207 additional information
-
 low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously Phaseolus vulgaris
2.4.1.207 additional information
-
 low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously Pisum sativum
2.4.1.207 additional information
-
 low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously Lens culinaris
2.4.1.207 additional information
-
 low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously Tamarindus indica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Phaseolus vulgaris ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Lens culinaris ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Tamarindus indica ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan acceptors: polymer xyloglucan or its derived oligosaccharides Tropaeolum majus ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan acceptor: fucosylated xyloglucan nonasaccharide Tropaeolum majus ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Phaseolus vulgaris ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Lens culinaris ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Tamarindus indica ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan xyloglucan from Tamarindus indica Tropaeolum majus ?
-
 ?
2.4.1.207 donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
-
 Phaseolus vulgaris ?
-
 ?
2.4.1.207 donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
-
 Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc Glc3Xyl2 Tropaeolum majus ?
-
 ?
2.4.1.207 donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
-
 Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc Glc4Xyl3Gal2 Tropaeolum majus ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination Phaseolus vulgaris ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination Pisum sativum ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination Lens culinaris ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination Tamarindus indica ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination Tropaeolum majus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.207 37
-
 assay at Phaseolus vulgaris
2.4.1.207 37
-
 assay at Pisum sativum
2.4.1.207 37
-
 assay at Lens culinaris
2.4.1.207 37
-
 assay at Tamarindus indica
2.4.1.207 37
-
 assay at Tropaeolum majus