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Literature summary extracted from

  • Schröder, R.; Atkinson, R.G.; Langenkämper, G.; Redgwell, R.J.
    Biochemical and molecular characterisation of xyloglucan endotransglycosylase from ripe kiwifruit (1998), Planta, 204, 242-251.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.207 6 isoforms, AdXET1-6 gene family is cloned, a full-length cDNA, AdXET5, is cloned, sequenced and overexpressed as active XET in Escherichia coli, purified XET is encoded by AdXET6 Actinidia deliciosa

General Stability

EC Number General Stability Organism
2.4.1.207 loss of activity if frozen and stored at -80°C Actinidia deliciosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.207 Ag+ 1 mM Actinidia deliciosa
2.4.1.207 Hg2+ at least 10 mM Actinidia deliciosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.207 additional information
-
 additional information Km: 0.6 mg/ml for kiwifruit xyloglucan Actinidia deliciosa
2.4.1.207 0.1
-
 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol reduced xyloglucan-derived heptasaccharide XXXG-ol, derived from kiwifruit xyloglucan Actinidia deliciosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.207 cell wall most of the XET activity is strongly associated with the cell wall Actinidia deliciosa 5618
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.207 34000
-
 x * 34000, predicted MW of the mature protein encoded by AdXET5 is 31.8 kDa, SDS-PAGE Actinidia deliciosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.207 donor xyloglucan + xyloglucan acceptor Actinidia deliciosa XET and xyloglucan may play a role in the cell wall changes that accompany fruit softening during ripening, substrates for XET action are located in the cell wall ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Actinidia deliciosa
-
 ripe kiwifruit, [A. Chev.] C.F. Liang et A.R. Ferguson var. deliciosa cv. Hayward, 6 isoenzymes: AdXET1-6 gene family
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.207 glycoprotein AdXET5 and AdXET6: asparagine-linked carbohydrate, N-glycosylated, glycosylation of the protein is not required for endo-transglycosylase activity Actinidia deliciosa
2.4.1.207 proteolytic modification 21 amino acids signal peptide at the N-terminus is cleaved to produce the mature protein Actinidia deliciosa

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.207 3000fold, AdXET6 Actinidia deliciosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 fruit core tissue of ripe kiwifruit Actinidia deliciosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.207 additional information
-
-
 Actinidia deliciosa

Storage Stability

EC Number Storage Stability Organism
2.4.1.207 -80°C, partially purified XET, ConA-eluate stage, 4 weeks, stable Actinidia deliciosa
2.4.1.207 -80°C, purified XET, loss of activity Actinidia deliciosa
2.4.1.207 4°C, purified XET, 24 h, stable Actinidia deliciosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 donor xyloglucan + acceptor xyloglucan kiwifruit xyloglucan donor, acceptor: XXXG-ol, a reduced heptasaccharide derived from kiwifruit xyloglucan Actinidia deliciosa ?
-
 ?
2.4.1.207 donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol XXXG-ol Actinidia deliciosa ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor XET and xyloglucan may play a role in the cell wall changes that accompany fruit softening during ripening, substrates for XET action are located in the cell wall Actinidia deliciosa ?
-
 ?
2.4.1.207 additional information in absence of reduced xyloglucan-derived heptasaccharide XXXG-ol enzyme depolymerizes xyloglucan by hydrolysis and in the presence of it by both hydrolysis and endotransglycosylation Actinidia deliciosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.207 ? x * 34000, predicted MW of the mature protein encoded by AdXET5 is 31.8 kDa, SDS-PAGE Actinidia deliciosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.207 22
-
 assay at Actinidia deliciosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.207 5.5 5.8
-
 Actinidia deliciosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.207 5 8 active between Actinidia deliciosa