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Literature summary extracted from
- Novel "dot-blot" assays for glycosyltransferases and glycosylhydrolases: Optimization for xyloglucan endotransglycosylase (XET) activity (1997), Plant J., 11, 1141-1150.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.207 | (NH4)2SO4 | 1 M, about 50% inhibition | Brassica oleracea |  |
| 2.4.1.207 | CaCl2 | 300 mM, about 50% inhibition | Brassica oleracea | |
| 2.4.1.207 | ethanol | 15%, about 3 M, about 50% inhibition of product formation | Brassica oleracea | |
| 2.4.1.207 | H2O2 | 1 M, about 50% inhibition | Brassica oleracea | |
| 2.4.1.207 | additional information | not inhibited by 1 M fluoride, 1 M urea, 1 M DMSO, 300 mM borate, 100 mM EDTA, 100 mM Triton X-100 | Brassica oleracea | |
| 2.4.1.207 | NaCl | 1 M, about 50% inhibition | Brassica oleracea | |
| 2.4.1.207 | NaN3 | 1 M, about 50% inhibition | Brassica oleracea | |
| 2.4.1.207 | phosphate | 300 mM, about 50% inhibition | Brassica oleracea | |
| 2.4.1.207 | Pyridine | 300 mM, about 50% inhibition | Brassica oleracea | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 2.4.1.207 | Ethanol | 15%, about 3 M, about 50% inhibition of product formation | Brassica oleracea |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.207 | Apium sp. | - |
celery | - |
| 2.4.1.207 | Arabidopsis sp. | - |
- |
- |
| 2.4.1.207 | Brassica oleracea | - |
cauliflower | - |
| 2.4.1.207 | Sinapis sp. | - |
mustard | - |
| 2.4.1.207 | Vigna radiata var. radiata | - |
mung bean | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.4.1.207 | petiole | activity is very pronounced in the vicinity of the vascular bundles | Apium sp. | - |
| 2.4.1.207 | seedling | - |
Vigna radiata var. radiata | - |
| 2.4.1.207 | seedling | - |
Sinapis sp. | - |
| 2.4.1.207 | sprout | - |
Vigna radiata var. radiata | - |
| 2.4.1.207 | stem | - |
Arabidopsis sp. | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.207 | additional information | - |
a rapid, semiquantitative assay suitable for testing crude plant extracts directly on to the test paper | Vigna radiata var. radiata |
| 2.4.1.207 | additional information | - |
a rapid, semiquantitative assay suitable for testing crude plant extracts directly on to the test paper | Arabidopsis sp. |
| 2.4.1.207 | additional information | - |
a rapid, semiquantitative assay suitable for testing crude plant extracts directly on to the test paper | Brassica oleracea |
| 2.4.1.207 | additional information | - |
a rapid, semiquantitative assay suitable for testing crude plant extracts directly on to the test paper | Sinapis sp. |
| 2.4.1.207 | additional information | - |
a rapid, semiquantitative assay suitable for testing crude plant extracts directly on to the test paper | Apium sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Vigna radiata var. radiata | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Arabidopsis sp. | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Brassica oleracea | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Sinapis sp. | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Apium sp. | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + acceptor xyloglucan | xyloglucan from Tropaeolum majus seed | Arabidopsis sp. | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate | XLLGol-SR | Vigna radiata var. radiata | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate | XLLGol-SR | Arabidopsis sp. | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate | XLLGol-SR | Brassica oleracea | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate | XLLGol-SR | Sinapis sp. | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate | XLLGol-SR | Apium sp. | ? | - |
? | |
| 2.4.1.207 | donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc | XLLG | Arabidopsis sp. | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.207 | 20 | - |
assay at | Vigna radiata var. radiata |
| 2.4.1.207 | 20 | - |
assay at | Arabidopsis sp. |
| 2.4.1.207 | 20 | - |
assay at | Brassica oleracea |
| 2.4.1.207 | 20 | - |
assay at | Sinapis sp. |
| 2.4.1.207 | 20 | - |
assay at | Apium sp. |
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