EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.3.3 | SIATL1 gene encoding STM is mapped to the long arm of chromosome 17 at q23-qter, full-length cDNA from normal human mammary epithelial cell strain 76N is cloned, sequenced and encodes a protein of 374 amino acids, expression in COS-7 cells | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.3.3 | 42000 | - |
x * 42000, predicted from the amino acid sequence | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.3 | CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | Homo sapiens | STM catalyzes the synthesis of O-glycans, SIATL1 gene encoding STM is widely expressed in normal human tissues, as well as in normal breast and prostate epithelial cells, but significantly down-regulated or absent in corresponding tumor cell lines | CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.3.3 | Homo sapiens | Q9UJ37 | STM, i.e. hST6GalNAc-II | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.4.3.3 | additional information | amino acid sequence of STM has a potential site for casein kinase II phosphorylation and 3 consensus motifs for N-glycosylation at positions Asn-85, Asn-130 and Asn-161 | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.3.3 | mammary epithelium | normal human mammary epithelial cell strains 70N, 76N and 81N, STM RNA expression is highest in 76N, followed by 81N and 70N | Homo sapiens | - |
2.4.3.3 | additional information | STM tissue distribution, SIATL1 gene encoding STM is widely expressed in normal human tissues, as well as in normal breast and prostate epithelial cells, but significantly down-regulated or absent in corresponding tumor cell lines, STM gene is down-regulated in T-47D, BT-474 and MDA-MB-435 breast tumor cell lines as compared to normal mammary epithelial cell strains, only RNA traces in other tumor cell lines tested, e.g. MDA-231, MDA-468 and MCF-7, only MDA-MB-361 metastatic tumor cell line expresses STM mRNA levels as high as in 76N | Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.3 | CMP-N-acetylneuraminate + asialo-fetuin | STM, i.e. hST6GalNAc-II, more effective than native fetuin | Homo sapiens | CMP + fetuin | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | STM catalyzes the synthesis of O-glycans, SIATL1 gene encoding STM is widely expressed in normal human tissues, as well as in normal breast and prostate epithelial cells, but significantly down-regulated or absent in corresponding tumor cell lines | Homo sapiens | CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + native fetuin | STM, i.e. hST6GalNAc-II, 83% as effective as asialo-fetuin | Homo sapiens | ? | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | acceptor substrate specificity | Homo sapiens | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | asialo-fetuin | Homo sapiens | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | Galbeta(1-3)GalNAc-O-Ser/Thr or Neu5Acalpha(2-3)Galbeta(1-3)GalNAc-O-Ser/Thr acceptor specificity | Homo sapiens | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | native fetuin | Homo sapiens | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | additional information | STM contains 2 catalytic domains, the L-sialylmotif, aa 148-194, and S-sialylmotif, aa 303-324, no acceptors: asialo-BSM, alpha1 acid glycoprotein, glycosphingolipids | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.3.3 | ? | x * 42000, predicted from the amino acid sequence | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.3.3 | STM | i.e. human ST6GalNAc-II | Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.3.3 | 37 | - |
assay at | Homo sapiens |