EC Number | General Stability | Organism |
---|---|---|
2.4.3.3 | 50 mM NaCl stabilizes during purification | Sus scrofa |
2.4.3.3 | glycerol, 50% w/v, stabilizes during storage | Sus scrofa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.3.3 | Antifreeze glycoprotein | competitive to ovine submaxillary asialo-mucin, non-competitive to CMP-NeuAc | Sus scrofa | |
2.4.3.3 | CTP | competitive to CMP-NeuAc | Sus scrofa | |
2.4.3.3 | additional information | not inhibited by EDTA; not inhibited by Mg2+; not inhibited by Zn2+, Mn2+ | Sus scrofa | |
2.4.3.3 | Triton X-100 | above critical micelle concentration of 0.016%, 15% inhibition, reversible | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.3 | 0.52 | - |
CMP-N-acetylneuraminate | pH 6.5, 37°C, antifreeze glycoprotein 8 as acceptor | Sus scrofa | |
2.4.3.3 | 0.66 | - |
CMP-N-acetylneuraminate | pH 6.5, 37°C, ovine submaxillary asialo-mucin as acceptor | Sus scrofa | |
2.4.3.3 | 1.02 | - |
Antifreeze glycoprotein | antifreeze glycoprotein 8, pH 6.5, 37°C | Sus scrofa | |
2.4.3.3 | 2.93 | - |
submaxillary asialo-mucin | ovine, pH 6.5, 37°C | Sus scrofa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.3.3 | membrane | - |
Sus scrofa | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.3.3 | additional information | no metal ion requirement, e.g. Mn2+, Mg2+ or Zn2+ | Sus scrofa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.3.3 | 100000 | 172000 | enzyme exists in several active molecular weight forms from 100000-172000, gel filtration or PAGE | Sus scrofa |
2.4.3.3 | 160000 | - |
x * 160000, enzyme consists of several electrophoretic forms, 160 kDa: largest one, SDS-PAGE | Sus scrofa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.3 | CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | Sus scrofa | involved in biosynthesis of O-linked oligosaccharide chains of glycoproteins | CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.3.3 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.3.3 | solubilized with Triton X-100, CDP-agarose affinity chromatography, 117000fold | Sus scrofa |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.4.3.3 | CMP-N-acetylneuraminate + glycano-(1->3)-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein = CMP + glycano-[(2->6)-alpha-N-acetylneuraminyl]-(N-acetyl-D-galactosaminyl)-glycoprotein | mechanism | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.3.3 | submaxillary gland | - |
Sus scrofa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.3.3 | 44.6 | - |
- |
Sus scrofa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.3 | CMP-N-acetylneuraminate + antifreeze glycoprotein | antifreeze glycoprotein 8, best acceptor | Sus scrofa | ? | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + asialo-fetuin | good substrate | Sus scrofa | CMP + fetuin | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | involved in biosynthesis of O-linked oligosaccharide chains of glycoproteins | Sus scrofa | CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | acceptor substrate specificity | Sus scrofa | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | antifreeze glycoprotein is the best substrate | Sus scrofa | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | R: H or a beta-galactoside | Sus scrofa | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | asialo-fetuin | Sus scrofa | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | specific for alpha-N-acetylgalactosamine attached through alpha-glycosidic linkage to threonine or serine in polypeptide chain | Sus scrofa | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | porcine submaxillary asialo-mucin | Sus scrofa | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | antifreeze glycoprotein | Sus scrofa | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser | Sus scrofa | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
2.4.3.3 | CMP-N-acetylneuraminate + submaxillary asialo-mucin | ovine and porcine submaxillary asialo-mucin are good acceptors | Sus scrofa | CMP + submaxillary mucin | linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser | ? | |
2.4.3.3 | additional information | no acceptors: glycoproteins with Asn-linked oligosaccharides, glycosides, mono- and oligosaccharides | Sus scrofa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.3.3 | ? | x * 160000, enzyme consists of several electrophoretic forms, 160 kDa: largest one, SDS-PAGE | Sus scrofa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.3.3 | 37 | - |
assay at | Sus scrofa |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.3 | 0.92 | - |
Antifreeze glycoprotein | pH 6.5, 37°C | Sus scrofa |