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Literature summary extracted from

  • Wang, C.; Luosujärvi, H.; Heikkinen, J.; Risteli, M.; Uitto, L.; Myllylä, R.
    The third activity for lysyl hydroxylase 3: galactosylation of hydroxylysyl residues in collagens in vitro (2002), Matrix Biol., 21, 559-566.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.66 a single gene codes for LH, GGT and GT activities Homo sapiens
2.4.1.66 LH cDNA with GGT activity is cloned and expressed in Escherichia coli BL21 (DE3) pLysS, a single gene codes for LH, GGT and GT activities Caenorhabditis elegans
2.4.1.66 LH3 cDNA with GGT activity is cloned and expressed in Sf9 insect cells and in Escherichia coli XL1-Blue Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.66 C144I mutant with dramatically reduced GGT activity Homo sapiens
2.4.1.66 additional information mutation of aspartates in the positions 187-191 significantly reduces GGT activity of LH3 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.66 0.017
-
 UDP-glucose recombinant LH3/GGT Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.66 soluble
-
 Caenorhabditis elegans
-
-
2.4.1.66 soluble LH3/GGT protein Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.66 81000
-
 x * 81000, LH3 with GGT activity, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen Homo sapiens posttranslational modification of collagen UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen Caenorhabditis elegans posttranslational modification of collagen UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.66 Caenorhabditis elegans
-
-
-
2.4.1.66 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.66 His-tagged recombinant LH3/GGT/GT protein expressed in Escherichia coli Homo sapiens
2.4.1.66 partial Homo sapiens
2.4.1.66 partial, His-tagged recombinant LH/GGT/GT protein expressed in Escherichia coli Caenorhabditis elegans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.66 additional information
-
-
 Homo sapiens
2.4.1.66 0.33
-
 GGT activity of LH3 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.66 additional information the amino acids important for the GGT activity are located in the N-terminal part of LH3/GGT protein separate from the C-terminal LH active site Homo sapiens ?
-
 ?
2.4.1.66 additional information multifunctional lysyl hydroxylase isoform 3, LH3 protein, possesses lysyl hydroxylase, collagen glucosyltransferase and galactosyltransferase activities Homo sapiens ?
-
 ?
2.4.1.66 additional information multifunctional lysyl hydroxylase possesses lysyl hydroxylase, collagen glucosyltransferase and galactosyltransferase activities Caenorhabditis elegans ?
-
 ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Caenorhabditis elegans UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen posttranslational modification of collagen Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen posttranslational modification of collagen Caenorhabditis elegans UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.66 ? x * 81000, LH3 with GGT activity, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.66 More GGT activity is associated with lysyl hydroxylase isoform 3, LH3, EC 1.14.11.4 Homo sapiens
2.4.1.66 More GGT activity is associated with lysyl hydroxylase, LH Caenorhabditis elegans
2.4.1.66 More cf. EC 2.4.1.50 Homo sapiens
2.4.1.66 More cf. EC 2.4.1.50 Caenorhabditis elegans