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Literature summary extracted from

  • Anttinen, H.; Kivirikko, K.I.
    Affinity chromatography of collagen glucosyltransferase on a UDP-glucose derivative coupled to agarose (1976), Biochim. Biophys. Acta, 429, 750-758.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.66 Mn2+ requirement Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.66 52000 54000 gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.66 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.66 3110fold Gallus gallus
2.4.1.66 affinity chromatography Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.66 embryo
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.66 0.046
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen peptides prepared by collagenase-digestion from bovine Achilles tendon collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen heat-denatured citrate-soluble rat skin collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen requires enzyme-Mn2+ for the binding of the substrate and co-substrate Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
2.4.1.66 UDP-glucose + D-galactosylhydroxylysine in vitro Gallus gallus UDP + 1,2-D-glucosyl-D-galactosylhydroxylysine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.66 37
-
 assay at Gallus gallus