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Literature summary extracted from

  • Anttinen, H.; Myllylä, R.; Kivirikko, K.I.
    Further characterization of galactosylhydroxylysyl glucosyltransferase from chick embryos. Amino acid composition and acceptor specificity (1978), Biochem. J., 175, 737-742.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.4.1.66 loses activity during purification mostly due to inactivation Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.66 2.5
-
 D-galactosylsphingosine
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.66 Mn2+
-
 Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.66 additional information
-
 amino acid composition Gallus gallus
2.4.1.66 70000
-
 about Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen Gallus gallus involved in collagen biosynthesis UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.66 Gallus gallus
-
 white Leghorn chicken
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.66 glycoprotein
-
 Gallus gallus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.66 39800fold from embryo Gallus gallus
2.4.1.66 affinity chromatography Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.66 embryo 14-15 days old Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.66 0.57
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.66 additional information not: casein, chondroitin sulfate, ovomucoid, alpha1-glycoprotein, alpha-lactalbumin, thyroglobulin Gallus gallus ?
-
 ?
2.4.1.66 additional information not: ovalbumin, transferrin, fetuin Gallus gallus ?
-
 ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen acceptor specificity Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen high specificity for galactosylhydroxylysine Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen heat-denatured citrate-soluble rat skin collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen specific for collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen involved in collagen biosynthesis Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
2.4.1.66 UDP-glucose + D-galactosylsphingosine in vitro Gallus gallus UDP + 1,2-D-glucosyl-D-galactosylsphingosine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.66 additional information
-
 additional information 0.7 mol of glucosylgalactosylhydroxylysine residues formed per s per mol of enzyme Gallus gallus