EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.21 | expression of wild-type, L201A and L201W mutant enzymes in Escherichia coli | Bacillus sp. (in: Bacteria) |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.6.1.21 | L201A | 2% of wild-type kcat | Bacillus sp. (in: Bacteria) |
2.6.1.21 | L201W | 0.043% of wild-type kcat | Bacillus sp. (in: Bacteria) |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.21 | additional information | L201A and L201W mutant enzymes lose their activity by incubation with D-alanine with biphasic kinetics | Bacillus sp. (in: Bacteria) |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.21 | 0.63 | - |
2-oxoglutarate | pH 8.0, 37°C, wild-type enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 0.67 | - |
2-oxoglutarate | pH 8.0, 37°C, L201W mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 1.2 | - |
D-alanine | pH 8.0, 37°C, L201W mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 1.5 | - |
D-alanine | pH 8.0, 37°C, L201A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 4.8 | - |
2-oxoglutarate | pH 8.0, 37°C, L201A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 20 | - |
D-alanine | pH 8.0, 37°C, wild-type enzyme | Bacillus sp. (in: Bacteria) |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.21 | Bacillus sp. (in: Bacteria) | - |
YM-1, thermophile | - |
2.6.1.21 | Bacillus sp. (in: Bacteria) YM-1 | - |
YM-1, thermophile | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.21 | wild-type, L201A and L201W mutant enzyme, DEAE-toyopearl | Bacillus sp. (in: Bacteria) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.21 | D-alanine + 2-oxoglutarate | - |
Bacillus sp. (in: Bacteria) | pyruvate + D-glutamate | - |
r | |
2.6.1.21 | D-alanine + 2-oxoglutarate | - |
Bacillus sp. (in: Bacteria) YM-1 | pyruvate + D-glutamate | - |
r |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.21 | 0.12 | - |
D-alanine | pH 8.0, 37°C, L201W mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 5.7 | - |
D-alanine | pH 8.0, 37°C, L201A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 280 | - |
D-alanine | pH 8.0, 37°C, wild-type enzyme | Bacillus sp. (in: Bacteria) |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.21 | pyridoxal 5'-phosphate | wild-type, L201A and L201W mutant enzyme: 0.89, 0.78 and 0.56 mol per mol of subunit, respectively | Bacillus sp. (in: Bacteria) |