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Literature summary extracted from

  • Tamaki, N.; Aoyama, H.; Kubo, K.; Ikeda, T.; Hama, T.
    Purification and properties of beta-alanine aminotransferase from rabbit liver (1982), J. Biochem., 92, 1009-1017.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.2 2-oxoglutarate substrate inhibition at neutral pH, not seen at pH 8.8 Oryctolagus cuniculus
2.6.1.2 6-Azauracil 1.5 mM, 50% inhibition Oryctolagus cuniculus
2.6.1.2 additional information not inhibited by 6-azauridine or 6-azauridine 5'-phosphate Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.2 0.51
-
 4-aminobutanoate pH 8.8, 37°C, cosubstrate 2-oxoglutarate Oryctolagus cuniculus
2.6.1.2 0.54
-
 2-oxoglutarate pH 8.8, 37°C Oryctolagus cuniculus
2.6.1.2 1.4
-
 2-oxoglutarate pH 8.8, 37°C, cosubstrate beta-alanine Oryctolagus cuniculus
2.6.1.2 3.9
-
 beta-Alanine pH 8.8, 37°C, cosubstrate 2-oxoglutarate Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.2 48000
-
 2 * 48000, SDS-PAGE Oryctolagus cuniculus
2.6.1.2 95000
-
 gel filtration Oryctolagus cuniculus
2.6.1.2 118000
-
 sucrose density gradient centrifugation Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.2 L-alanine + 2-oxoglutarate Oryctolagus cuniculus important function in beta-alanine metabolism pyruvate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Oryctolagus cuniculus
-
-
-
2.6.1.19 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.2 heat treatment, ammonium sulfate, DEAE-Sepharose, hydroxylapatite, Sephacryl S-200 Oryctolagus cuniculus
2.6.1.19
-
 Oryctolagus cuniculus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.2 L-alanine + 2-oxoglutarate = pyruvate + L-glutamate ping pong bi bi mechanism Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.2 liver
-
 Oryctolagus cuniculus
-
2.6.1.19 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.2 1.58
-
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.6.1.2 -25°C, 10 mM potassium phosphate, pH 7.5, 1 mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal 5'-phosphate, a few days, 50% loss of activity Oryctolagus cuniculus
2.6.1.2 4°C, 80% saturated solution of ammonium sulfate, 10 days, 40% loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 2-oxoglutarate + 5-aminopentanoate 121% of the activity with beta-alanine Oryctolagus cuniculus glutamate + 5-oxopentanoate
-
 ?
2.6.1.2 2-oxoglutarate + 6-aminohexanoate 8% of the activity with beta-alanine Oryctolagus cuniculus glutamate + 6-oxohexanoate
-
 ?
2.6.1.2 2-oxoglutarate + DL-3-aminoisobutanoate 51% of the activity with beta-alanine Oryctolagus cuniculus glutamate + 3-oxoisobutanoate
-
 ?
2.6.1.2 4-aminobutanoate + 2-oxoglutarate at 131% of the activity with beta-alanine Oryctolagus cuniculus 4-oxobutanoate + glutamate
-
 ?
2.6.1.2 beta-alanine + glyoxylic acid 7% of the activity with 2-oxoglutarate Oryctolagus cuniculus malonic semialdehyde + glycine
-
 ?
2.6.1.2 beta-alanine + pyruvate 1% of the activity with 2-oxoglutarate Oryctolagus cuniculus malonic semialdehyde + alanine
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Oryctolagus cuniculus pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate important function in beta-alanine metabolism Oryctolagus cuniculus pyruvate + L-glutamate
-
 ?
2.6.1.19 4-aminobutanoate + 2-oxoglutarate
-
 Oryctolagus cuniculus 4-oxobutanoate + L-glutamate
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.2 dimer 2 * 48000, SDS-PAGE Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.2 8.8
-
-
 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.2 pyridoxal 5'-phosphate 1 mol of pyridoxal 5'-phosphate per mol of dimer Oryctolagus cuniculus