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Literature summary extracted from

  • Hatch, M.D.
    Separation and properties of leaf aspartate aminotransferase and alanine aminotransferase isoenzymes operative in the C4 pathway of photosynthesis (1973), Arch. Biochem. Biophys., 156, 207-214.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.1 Hadacidin i.e. N-formylhydroxyaminoacetic acid Atriplex spongiosa
2.6.1.1 malate oxoglutarate production, oxaloacetate production Atriplex spongiosa
2.6.1.2 malate 5 mM, 25% inhibition Atriplex spongiosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.1 0.045
-
 oxaloacetate isozyme Asp-DEAE-2, pH 8.0 Atriplex spongiosa
2.6.1.1 0.08
-
 2-oxoglutarate isozyme Asp-DEAE-1, pH 8.0 Atriplex spongiosa
2.6.1.1 0.1
-
 oxaloacetate isozyme Asp-DEAE-1, pH 8.0 Atriplex spongiosa
2.6.1.1 0.12
-
 2-oxoglutarate isozyme Asp-DEAE-2, pH 8.0 Atriplex spongiosa
2.6.1.1 0.5
-
 L-aspartate isozyme Asp-DEAE-2, pH 8.0 Atriplex spongiosa
2.6.1.1 0.75
-
 L-glutamate isozyme Asp-DEAE-2, pH 8.0 Atriplex spongiosa
2.6.1.1 0.85
-
 L-aspartate isozyme Asp-DEAE-1, pH 8.0 Atriplex spongiosa
2.6.1.1 1.6
-
 L-glutamate isozyme Asp-DEAE-1, pH 8.0 Atriplex spongiosa
2.6.1.2 0.022
-
 pyruvate pH 7.5, 22°C, isoenzyme 3 Atriplex spongiosa
2.6.1.2 0.028
-
 2-oxoglutarate pH 7.5, 22°C, isoenzyme 3 Atriplex spongiosa
2.6.1.2 0.04
-
 pyruvate pH 7.5, 22°C, isoenzyme 2 Atriplex spongiosa
2.6.1.2 0.09
-
 2-oxoglutarate pH 7.5, 22°C, isoenzyme 2 Atriplex spongiosa
2.6.1.2 0.25
-
 alanine pH 7.5, 22°C, isoenzyme 1 Atriplex spongiosa
2.6.1.2 0.3
-
 2-oxoglutarate pH 7.5, 22°C, isoenzyme 2 Atriplex spongiosa
2.6.1.2 0.45
-
 pyruvate pH 7.5, 22°C, isoenzyme 1 Atriplex spongiosa
2.6.1.2 0.55
-
 glutamate pH 7.5, 22°C, isoenzyme 1 Atriplex spongiosa
2.6.1.2 0.8
-
 glutamate pH 7.5, 22°C, isoenzyme 3 Atriplex spongiosa
2.6.1.2 1.15
-
 glutamate pH 7.5, 22°C, isoenzyme 2 Atriplex spongiosa
2.6.1.2 3
-
 alanine pH 7.5, 22°C, isoenzyme 2 Atriplex spongiosa
2.6.1.2 3.1
-
 alanine pH 7.5, 22°C, isoenzyme 3 Atriplex spongiosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate Atriplex spongiosa
-
 oxaloacetate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Atriplex spongiosa
-
 2 major isozymes: Asp-DEAE-1 and Asp-DEAE-2
-
2.6.1.2 Atriplex spongiosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 isoenzymes: Asp-DEAE-1 and Asp-DEAE-2 Atriplex spongiosa
2.6.1.2 ammonium sulfate, DEAE-cellulose, 3 isoforms Atriplex spongiosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.1 bundle sheath isozyme Asp-DEAE-2 Atriplex spongiosa
-
2.6.1.1 leaf
-
 Atriplex spongiosa
-
2.6.1.1 mesophyll isozyme Asp-DEAE-1 Atriplex spongiosa
-
2.6.1.2 leaf
-
 Atriplex spongiosa
-

Storage Stability

EC Number Storage Stability Organism
2.6.1.1 0°C, stable for at least several h in solution Atriplex spongiosa
2.6.1.2 0°C, stable for several h in solution Atriplex spongiosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Atriplex spongiosa oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + 2-oxoglutarate isozyme Asp-DEAE-1 performs preferably the reverse reaction, isozyme the forward reaction Atriplex spongiosa oxaloacetate + L-glutamate
-
 r
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Atriplex spongiosa pyruvate + L-glutamate
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.2 22
-
 isoenzymes 2 and 3, 10-20% loss of activity after 3 h Atriplex spongiosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 6.8 8.3 formation of oxaloacetate, isoenzyme Asp-DEAE-1 Atriplex spongiosa
2.6.1.1 8
-
 oxaloacetate formation, isoenzyme Asp-DEAE-2 Atriplex spongiosa
2.6.1.2 7.5
-
-
 Atriplex spongiosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.2 6.4 8.7 at least 50% of maximal activity at pH 6.4 and 8.7 Atriplex spongiosa