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Literature summary extracted from

  • Kim, A.D.; Baker, A.S.; Dunaway-Mariano, D.; Metcalf, W.W.; Wanner, B.L.; Martin, B.M.
    The 2-aminoethylphosphonate-specific transaminase of the 2-aminoethylphosphonate degradation pathway (2002), J. Bacteriol., 184, 4134-4140.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.37 expression in Escherichia coli Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.37 D168A no activity Salmonella enterica
2.6.1.37 K194L no activity Salmonella enterica
2.6.1.37 K194R no activity Salmonella enterica
2.6.1.37 R340A partial activity Salmonella enterica
2.6.1.37 R340K partial activity Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.37 0.009
-
 2-phosphonoacetaldehyde pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate Salmonella enterica
2.6.1.37 0.15
-
 pyruvate pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde Salmonella enterica
2.6.1.37 0.19
-
 2-phosphonoacetaldehyde pH 8.5, 25°C, R340A mutant enzyme Salmonella enterica
2.6.1.37 0.5
-
 pyruvate pH 8.5, 25°C, R340K mutant enzyme Salmonella enterica
2.6.1.37 1.11
-
 (2-aminoethyl)phosphonate pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde Salmonella enterica
2.6.1.37 1.4
-
 L-alanine pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate Salmonella enterica
2.6.1.37 2.8
-
 D-alanine pH 8.5, 25°C, R340K mutant enzyme Salmonella enterica
2.6.1.37 2.9
-
 2-phosphonoacetaldehyde pH 8.5, 25°C, R340K mutant enzyme Salmonella enterica
2.6.1.37 3.7
-
 D-alanine pH 8.5, 25°C, R340A mutant enzyme Salmonella enterica
2.6.1.37 6.1
-
 pyruvate pH 8.5, 25°C, R340A mutant enzyme Salmonella enterica
2.6.1.37 11
-
 D-alanine pH 8.5, 25°C Salmonella enterica
2.6.1.37 20
-
 L-alanine pH 8.5, 25°C, R340K mutant enzyme Salmonella enterica
2.6.1.37 26
-
 2-aminoethylphosphonate pH 8.5, 25°C, R340A mutant enzyme Salmonella enterica
2.6.1.37 140
-
 L-alanine pH 8.5, 25°C, R340A mutant enzyme Salmonella enterica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.37 16500
-
 4 * 16500, SDS-PAGE Pseudomonas aeruginosa
2.6.1.37 42000
-
 2 * 42000, SDS-PAGE Salmonella enterica
2.6.1.37 100000
-
 gel filtration Salmonella enterica

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.37 Pseudomonas aeruginosa Q51573 encoded by phnW gene
-
2.6.1.37 Salmonella enterica
-
 serovar Thyphimurium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.37 recombinant enzyme, amonium sulfate, DEAE-cellulose Salmonella enterica

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.37 (2-aminoethyl)phosphonate + pyruvate = 2-phosphonoacetaldehyde + L-alanine bi-bi ping-pong mechanism Salmonella enterica
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.37 (2-aminoethyl)phosphonate + pyruvate highly specific for (2-aminoethyl)phosphonate, no activity with oxaloacetate as NH3 donor Salmonella enterica 2-phosphonoacetaldehyde + L-alanine
-
 r
2.6.1.37 2-phosphonoacetaldehyde + D-alanine L-alanine is preferred but not absolutely required Salmonella enterica (2-aminoethyl)phosphonate + pyruvate
-
 ?
2.6.1.37 alpha-ketoglutarate + L-alanine 0.25% of the kcat observed with 2-phosphonoacetaldehyde Salmonella enterica L-glutamate + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.37 dimer 2 * 42000, SDS-PAGE Salmonella enterica
2.6.1.37 tetramer 4 * 16500, SDS-PAGE Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.37 0.02
-
 (2-aminoethyl)phosphonate pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde, R340K and R340A mutant enzymes Salmonella enterica
2.6.1.37 0.02
-
 2-phosphonoacetaldehyde pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate, R340A mutant enzyme Salmonella enterica
2.6.1.37 0.04
-
 D-alanine pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate Salmonella enterica
2.6.1.37 0.6
-
 2-phosphonoacetaldehyde pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate, R340K mutant enzyme Salmonella enterica
2.6.1.37 7.4
-
 (2-aminoethyl)phosphonate pH 8.5, 25°C, synthesis of 2-phosphonoacetaldehyde Salmonella enterica
2.6.1.37 9.3
-
 L-alanine pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate Salmonella enterica
2.6.1.37 9.3
-
 2-phosphonoacetaldehyde pH 8.5, 25°C, synthesis of (2-aminoethyl)phosphonate Salmonella enterica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.37 6.5 9.5 synthesis of 2-phosphonoacetaldehyde Salmonella enterica
2.6.1.37 7.5 8.5 synthesis of (2-aminoethyl)phosphonate Salmonella enterica