BRENDA - Enzyme Database show

Purification and properties of 2-aminoethylphosphonate:pyruvate aminotransferase from Pseudomonas aeruginosa

Dumora, C.; Lacoste, A.M.; Cassaigne, A.; Eur. J. Biochem. 133, 119-125 (1983)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.37
4-aminobutyrate
inhibitor/substrate ratio = 3, 8% inhibition
Pseudomonas aeruginosa
2.6.1.37
acetate
inhibitor/substrate ratio = 3, 20% inhibition
Pseudomonas aeruginosa
2.6.1.37
Aminomethylphosphonate
inhibitor/substrate ratio = 3, 50% inhibition
Pseudomonas aeruginosa
2.6.1.37
Aminomethylsulfonate
inhibitor/substrate ratio = 3, 20% inhibition
Pseudomonas aeruginosa
2.6.1.37
Aminooxyacetate
1 mM, 100% inhibition
Pseudomonas aeruginosa
2.6.1.37
beta-Alanine
inhibitor/substrate ratio = 3, 20% inhibition
Pseudomonas aeruginosa
2.6.1.37
D-cycloserine
1 mM, 40% inhibition
Pseudomonas aeruginosa
2.6.1.37
DL-1-Aminobutylphosphonate
inhibitor/substrate ratio = 3, 22% inhibition
Pseudomonas aeruginosa
2.6.1.37
DL-1-Aminopentylphosphonate
inhibitor/substrate ratio = 3, 22% inhibition
Pseudomonas aeruginosa
2.6.1.37
ethylphosphonate
inhibitor/substrate ratio = 3, 94% inhibition
Pseudomonas aeruginosa
2.6.1.37
ethylsulfonate
inhibitor/substrate ratio = 3, 12% inhibition
Pseudomonas aeruginosa
2.6.1.37
HgCl2
1 mM, 100% inhibition
Pseudomonas aeruginosa
2.6.1.37
hydroxylamine
1 mM, 50% inhibition
Pseudomonas aeruginosa
2.6.1.37
L-cysteine
10 mM, 35% inhibition
Pseudomonas aeruginosa
2.6.1.37
methylphosphonate
inhibitor/substrate ratio = 3, 90% inhibition
Pseudomonas aeruginosa
2.6.1.37
phenylhydrazine
1 mM, 20% inhibition
Pseudomonas aeruginosa
2.6.1.37
Propylphosphonate
inhibitor/substrate ratio = 3, 65% inhibition
Pseudomonas aeruginosa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.37
3.5
-
pyruvate
pH 8.5, 30C
Pseudomonas aeruginosa
2.6.1.37
3.85
-
2-aminoethylphosphonate
pH 8.5, 30C
Pseudomonas aeruginosa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.37
16500
-
4 * 16500, SDS-PAGE
Pseudomonas aeruginosa
2.6.1.37
65000
-
gel filtration
Pseudomonas aeruginosa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.37
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.37
poly(ethyleneimine), heat treatment, ammonium sulfate, DEAE-cellulose, hydroxyapatite, Ultrogel AcA
Pseudomonas aeruginosa
Reaction
EC Number
Reaction
Commentary
Organism
2.6.1.37
(2-aminoethyl)phosphonate + pyruvate = 2-phosphonoacetaldehyde + L-alanine
ping-pong bi-bi mechanism
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.6.1.37
16.64
-
-
Pseudomonas aeruginosa
Storage Stability
EC Number
Storage Stability
Organism
2.6.1.37
-20C, several months, no loss of activity
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.37
(2-aminoethyl)phosphonate + pyruvate
highly specific for pyruvate and (2-aminoethyl)phosphonate, no activity with D-penicillamine, N-ethylmaleimide, iodoacetamide, 3-aminopropylphosphonate and taurine
636706
Pseudomonas aeruginosa
2-phosphonoacetaldehyde + L-alanine
-
636706
Pseudomonas aeruginosa
?
2.6.1.37
(2-aminoethyl)phosphonate + pyruvate
trivial name ciliatine
636706
Pseudomonas aeruginosa
2-phosphonoacetaldehyde + L-alanine
-
636706
Pseudomonas aeruginosa
?
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.37
tetramer
4 * 16500, SDS-PAGE
Pseudomonas aeruginosa
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.6.1.37
50
-
-
Pseudomonas aeruginosa
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.6.1.37
70
-
inactivation
Pseudomonas aeruginosa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.37
8.5
9
-
Pseudomonas aeruginosa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.6.1.37
7.3
10.8
approx. 20% of maximal activity at pH 7.3, approx. 60% of maximal activity at pH 10.8, no activity above pH 11.5
Pseudomonas aeruginosa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.37
pyridoxal 5'-phosphate
a pyridoxal phosphate protein, 4 mol of pyridoxal phosphate per mol of enzyme
Pseudomonas aeruginosa
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.6.1.37
1
-
ethylphosphonate
-
Pseudomonas aeruginosa
2.6.1.37
1.55
-
methylphosphonate
-
Pseudomonas aeruginosa
2.6.1.37
2.8
-
Propylphosphonate
-
Pseudomonas aeruginosa
2.6.1.37
3.85
-
Aminomethylphosphonate
-
Pseudomonas aeruginosa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.37
pyridoxal 5'-phosphate
a pyridoxal phosphate protein, 4 mol of pyridoxal phosphate per mol of enzyme
Pseudomonas aeruginosa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.37
4-aminobutyrate
inhibitor/substrate ratio = 3, 8% inhibition
Pseudomonas aeruginosa
2.6.1.37
acetate
inhibitor/substrate ratio = 3, 20% inhibition
Pseudomonas aeruginosa
2.6.1.37
Aminomethylphosphonate
inhibitor/substrate ratio = 3, 50% inhibition
Pseudomonas aeruginosa
2.6.1.37
Aminomethylsulfonate
inhibitor/substrate ratio = 3, 20% inhibition
Pseudomonas aeruginosa
2.6.1.37
Aminooxyacetate
1 mM, 100% inhibition
Pseudomonas aeruginosa
2.6.1.37
beta-Alanine
inhibitor/substrate ratio = 3, 20% inhibition
Pseudomonas aeruginosa
2.6.1.37
D-cycloserine
1 mM, 40% inhibition
Pseudomonas aeruginosa
2.6.1.37
DL-1-Aminobutylphosphonate
inhibitor/substrate ratio = 3, 22% inhibition
Pseudomonas aeruginosa
2.6.1.37
DL-1-Aminopentylphosphonate
inhibitor/substrate ratio = 3, 22% inhibition
Pseudomonas aeruginosa
2.6.1.37
ethylphosphonate
inhibitor/substrate ratio = 3, 94% inhibition
Pseudomonas aeruginosa
2.6.1.37
ethylsulfonate
inhibitor/substrate ratio = 3, 12% inhibition
Pseudomonas aeruginosa
2.6.1.37
HgCl2
1 mM, 100% inhibition
Pseudomonas aeruginosa
2.6.1.37
hydroxylamine
1 mM, 50% inhibition
Pseudomonas aeruginosa
2.6.1.37
L-cysteine
10 mM, 35% inhibition
Pseudomonas aeruginosa
2.6.1.37
methylphosphonate
inhibitor/substrate ratio = 3, 90% inhibition
Pseudomonas aeruginosa
2.6.1.37
phenylhydrazine
1 mM, 20% inhibition
Pseudomonas aeruginosa
2.6.1.37
Propylphosphonate
inhibitor/substrate ratio = 3, 65% inhibition
Pseudomonas aeruginosa
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.6.1.37
1
-
ethylphosphonate
-
Pseudomonas aeruginosa
2.6.1.37
1.55
-
methylphosphonate
-
Pseudomonas aeruginosa
2.6.1.37
2.8
-
Propylphosphonate
-
Pseudomonas aeruginosa
2.6.1.37
3.85
-
Aminomethylphosphonate
-
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.37
3.5
-
pyruvate
pH 8.5, 30C
Pseudomonas aeruginosa
2.6.1.37
3.85
-
2-aminoethylphosphonate
pH 8.5, 30C
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.37
16500
-
4 * 16500, SDS-PAGE
Pseudomonas aeruginosa
2.6.1.37
65000
-
gel filtration
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.37
poly(ethyleneimine), heat treatment, ammonium sulfate, DEAE-cellulose, hydroxyapatite, Ultrogel AcA
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.6.1.37
16.64
-
-
Pseudomonas aeruginosa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.6.1.37
-20C, several months, no loss of activity
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.37
(2-aminoethyl)phosphonate + pyruvate
highly specific for pyruvate and (2-aminoethyl)phosphonate, no activity with D-penicillamine, N-ethylmaleimide, iodoacetamide, 3-aminopropylphosphonate and taurine
636706
Pseudomonas aeruginosa
2-phosphonoacetaldehyde + L-alanine
-
636706
Pseudomonas aeruginosa
?
2.6.1.37
(2-aminoethyl)phosphonate + pyruvate
trivial name ciliatine
636706
Pseudomonas aeruginosa
2-phosphonoacetaldehyde + L-alanine
-
636706
Pseudomonas aeruginosa
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.37
tetramer
4 * 16500, SDS-PAGE
Pseudomonas aeruginosa
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.6.1.37
50
-
-
Pseudomonas aeruginosa
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.6.1.37
70
-
inactivation
Pseudomonas aeruginosa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.37
8.5
9
-
Pseudomonas aeruginosa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.6.1.37
7.3
10.8
approx. 20% of maximal activity at pH 7.3, approx. 60% of maximal activity at pH 10.8, no activity above pH 11.5
Pseudomonas aeruginosa