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Literature summary extracted from

  • Mapson, L.W.; March, J.F.; Wardale, D.A.
    Biosynthesis of ethylene. 4-methylmercapto-2-oxobutyric acid: an intermediate in the formation from methionine (1969), Biochem. J., 115, 653-661.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.41 mitochondrion
-
 Brassica sp. 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.41 D-methionine + pyruvate Brassica sp. involved in ethylene synthesis from methionine 4-methylthio-2-oxobutanoate + L-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.41 Brassica sp.
-
 cauliflower
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.41 partial Brassica sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.41 floret
-
 Brassica sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.41 D-methionine + 2-oxoglutarate less effective than pyruvate Brassica sp. 4-methylthio-2-oxobutanoate + L-glutamate
-
 ?
2.6.1.41 D-methionine + oxaloacetate less effective than pyruvate Brassica sp. 4-methylthio-2-oxobutanoate + L-aspartate
-
 ?
2.6.1.41 D-methionine + pyruvate
-
 Brassica sp. 4-methylthio-2-oxobutanoate + L-alanine
-
 ?
2.6.1.41 D-methionine + pyruvate involved in ethylene synthesis from methionine Brassica sp. 4-methylthio-2-oxobutanoate + L-alanine
-
 ?
2.6.1.41 L-methionine + pyruvate one third of activity compared to D-methionine Brassica sp. 4-methylthio-2-oxobutanoate + L-alanine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.41 25
-
-
 Brassica sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.41 7.5
-
-
 Brassica sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.41 6 8 15% of maximal activity at pH 6.0, 66% of maximal activity at pH 8.0 Brassica sp.