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Literature summary extracted from

  • Yonaha, K.; Toyama, S.; Soda, K.
    Taurine-glutamate transaminase (1985), Methods Enzymol., 113, 102-108.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.55 ammonium sulfate at pH 7.0-7.6, crystals appear after 2-3 days Achromobacter superficialis

General Stability

EC Number General Stability Organism
2.6.1.55 treatment with ammonium sulfate during purification leads to a significant loss of activity Achromobacter superficialis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.55 3-Methyl-2-benzothiazolone hydrazone hydrochloride weak inhibition Achromobacter superficialis
2.6.1.55 D-cycloserine 0.5 mM; 20% inhibition Achromobacter superficialis
2.6.1.55 HgCl2
-
Achromobacter superficialis
2.6.1.55 hydroxylamine
-
Achromobacter superficialis
2.6.1.55 L-Cycloserine
-
Achromobacter superficialis
2.6.1.55 additional information not inhibited by p-chloromercuribenzoate, monoiodoacetic acid, D-penicillamine, L-penicillamine and EDTA Achromobacter superficialis
2.6.1.55 phenylhydrazine 0.5 mM, 17% inhibition Achromobacter superficialis
2.6.1.55 Semicarbazide 0.5 mM, 21% inhibition Achromobacter superficialis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.55 11
-
2-oxoglutarate pH 8.0, 37°C, cosubstrate DL-3-aminoisobutyrate Achromobacter superficialis
2.6.1.55 12
-
taurine pH 8.0, 37°C Achromobacter superficialis
2.6.1.55 16
-
Hypotaurine pH 8.0, 37°C Achromobacter superficialis
2.6.1.55 17
-
beta-Alanine pH 8.0, 37°C Achromobacter superficialis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.55 156000
-
sedimentation equilibrium Achromobacter superficialis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.55 Achromobacter polymorph
-
KR B-88
-
2.6.1.55 Achromobacter polymorph KR B-88
-
KR B-88
-
2.6.1.55 Achromobacter superficialis
-
ICR-B-89
-
2.6.1.55 Achromobacter superficialis ICR-B-89
-
ICR-B-89
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.55
-
Achromobacter superficialis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.55 0.519
-
-
Achromobacter superficialis

Storage Stability

EC Number Storage Stability Organism
2.6.1.55 0-5°C, crystalline enzyme in 10 mM potassium phosphate buffer, pH 7.0, 60% saturation of ammonium sulfate, 100 mM pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, several months, no loss of activity Achromobacter superficialis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.55 3-aminopropanesulfonate + 2-oxoglutarate
-
Achromobacter polymorph 2-oxopropanesulfonate + L-glutamate
-
?
2.6.1.55 3-aminopropanesulfonate + 2-oxoglutarate 43% of the activity with taurine Achromobacter superficialis 2-oxopropanesulfonate + L-glutamate
-
?
2.6.1.55 4-aminobutyrate + 2-oxoglutarate 60% of the activity with taurine Achromobacter superficialis L-glutamate + acetaldehyde + sulfite
-
?
2.6.1.55 beta-alanine + 2-oxoglutarate
-
Achromobacter polymorph 3-oxopropanoate + L-glutamate
-
?
2.6.1.55 beta-alanine + 2-oxoglutarate 184% of the activity with taurine Achromobacter superficialis 3-oxopropanoate + L-glutamate
-
?
2.6.1.55 beta-alanine + 2-oxoglutarate
-
Achromobacter polymorph KR B-88 3-oxopropanoate + L-glutamate
-
?
2.6.1.55 beta-alanine + 2-oxoglutarate 184% of the activity with taurine Achromobacter superficialis ICR-B-89 3-oxopropanoate + L-glutamate
-
?
2.6.1.55 DL-3-aminobutyrate + 2-oxoglutarate 14% of the activity with taurine Achromobacter superficialis 3-oxobutyrate + L-glutamate
-
?
2.6.1.55 DL-3-aminoisobutyrate + 2-oxoglutarate
-
Achromobacter polymorph 2-methyl-3-oxopropanoate + L-glutamate
-
?
2.6.1.55 DL-3-aminoisobutyrate + 2-oxoglutarate 208% of the activity with taurine Achromobacter superficialis 2-methyl-3-oxopropanoate + L-glutamate
-
?
2.6.1.55 DL-3-aminoisobutyrate + 2-oxoglutarate
-
Achromobacter polymorph KR B-88 2-methyl-3-oxopropanoate + L-glutamate
-
?
2.6.1.55 DL-3-aminoisobutyrate + 2-oxoglutarate 208% of the activity with taurine Achromobacter superficialis ICR-B-89 2-methyl-3-oxopropanoate + L-glutamate
-
?
2.6.1.55 hypotaurine + 2-oxoglutarate
-
Achromobacter polymorph 2-oxoethanesulfinic acid + L-glutamate
-
?
2.6.1.55 hypotaurine + 2-oxoglutarate 601% of the activity with taurine Achromobacter superficialis 2-oxoethanesulfinic acid + L-glutamate
-
?
2.6.1.55 hypotaurine + 2-oxoglutarate
-
Achromobacter polymorph KR B-88 2-oxoethanesulfinic acid + L-glutamate
-
?
2.6.1.55 taurine + 2-oxoglutarate no activity with aminomethanesulfonate, glycine, 5-aminopentanoate and amines Achromobacter superficialis sulfoacetaldehyde + L-glutamate
-
r
2.6.1.55 taurine + 2-oxoglutarate no activity with L- and D-amino acids Achromobacter polymorph sulfoacetaldehyde + L-glutamate
-
r
2.6.1.55 taurine + 2-oxoglutarate no activity with L- and D-amino acids Achromobacter superficialis sulfoacetaldehyde + L-glutamate
-
r
2.6.1.55 taurine + 2-oxoglutarate no activity with pyruvate, phenylpyruvate, oxaloacetate Achromobacter polymorph sulfoacetaldehyde + L-glutamate
-
r
2.6.1.55 taurine + 2-oxoglutarate no activity with pyruvate, phenylpyruvate, oxaloacetate Achromobacter superficialis sulfoacetaldehyde + L-glutamate
-
r
2.6.1.55 taurine + 2-oxoglutarate no activity with L- and D-amino acids Achromobacter polymorph KR B-88 sulfoacetaldehyde + L-glutamate
-
r
2.6.1.55 taurine + 2-oxoglutarate no activity with pyruvate, phenylpyruvate, oxaloacetate Achromobacter polymorph KR B-88 sulfoacetaldehyde + L-glutamate
-
r
2.6.1.55 taurine + 2-oxoglutarate no activity with aminomethanesulfonate, glycine, 5-aminopentanoate and amines Achromobacter superficialis ICR-B-89 sulfoacetaldehyde + L-glutamate
-
r
2.6.1.55 taurine + 2-oxoglutarate no activity with L- and D-amino acids Achromobacter superficialis ICR-B-89 sulfoacetaldehyde + L-glutamate
-
r
2.6.1.55 taurine + 2-oxoglutarate no activity with pyruvate, phenylpyruvate, oxaloacetate Achromobacter superficialis ICR-B-89 sulfoacetaldehyde + L-glutamate
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.55 60
-
-
Achromobacter superficialis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.55 7.8 8
-
Achromobacter superficialis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.55 pyridoxal 5'-phosphate Km: 0.005 mM Achromobacter superficialis
2.6.1.55 pyridoxal 5'-phosphate a pyridoxal phosphate protein Achromobacter polymorph
2.6.1.55 pyridoxal 5'-phosphate a pyridoxal phosphate protein Achromobacter superficialis