EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.57 | overexpression in Escherichia coli | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.57 | additional information | - |
additional information | Km values of half-reactions | Escherichia coli | |
2.6.1.57 | 0.26 | - |
L-phenylalanine | pH 8.0, 25°C | Escherichia coli | |
2.6.1.57 | 0.32 | - |
L-tyrosine | pH 8.0, 25°C, cosubstrate 2-oxoglutarate | Escherichia coli | |
2.6.1.57 | 0.5 | - |
L-tryptophan | pH 8.0, 25°C, cosubstrate 2-oxoglutarate | Escherichia coli | |
2.6.1.57 | 0.59 | - |
2-oxoglutarate | pH 8.0, 25°C, cosubstrate tryptophan | Escherichia coli | |
2.6.1.57 | 0.8 | - |
2-oxoglutarate | pH 8.0, 25°C, cosubstrate aspartate | Escherichia coli | |
2.6.1.57 | 1.3 | - |
2-oxoglutarate | pH 8.0, 25°C, cosubstrate tyrosine | Escherichia coli | |
2.6.1.57 | 1.7 | - |
2-oxoglutarate | pH 8.0, 25°C, cosubstrate phenylalanine | Escherichia coli | |
2.6.1.57 | 3.8 | - |
L-aspartate | pH 8.0, 25°C, cosubstrate 2-oxoglutarate | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 43000 | - |
2 * 43000, SDS-PAGE | Escherichia coli |
2.6.1.57 | 43537 | - |
2 * 43537, deduced from nucleotide sequence | Escherichia coli |
2.6.1.57 | 83000 | - |
gel filtration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.57 | Escherichia coli | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.57 | 170 | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.57 | aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
r | |
2.6.1.57 | L-phenylalanine + 2-oxoglutarate | - |
Escherichia coli | phenylpyruvate + L-glutamate | - |
? | |
2.6.1.57 | L-tryptophan + 2-oxoglutarate | - |
Escherichia coli | 3-indole-2-oxopropanoate + L-glutamate | - |
r | |
2.6.1.57 | L-tyrosine + 2-oxoglutarate | - |
Escherichia coli | p-hydroxyphenylpyruvate + L-glutamate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.57 | dimer | 2 * 43000, SDS-PAGE | Escherichia coli |
2.6.1.57 | dimer | 2 * 43537, deduced from nucleotide sequence | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.57 | 140 | - |
L-aspartate | pH 8.0, 25°C, cosubstrate 2-oxoglutarate | Escherichia coli | |
2.6.1.57 | 160 | - |
L-tryptophan | pH 8.0, 25°C, cosubstrate 2-oxoglutarate | Escherichia coli | |
2.6.1.57 | 210 | - |
L-tyrosine | pH 8.0, 25°C, cosubstrate 2-oxoglutarate | Escherichia coli | |
2.6.1.57 | 250 | - |
L-phenylalanine | pH 8.0, 25°C, cosubstrate 2-oxoglutarate | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.57 | pyridoxal 5'-phosphate | a pyridoxal phosphate protein | Escherichia coli |