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Literature summary extracted from

  • Noguchi, T.
    Aromatic-amino-acid aminotransferase from small intestine (1987), Methods Enzymol., 142, 267-273.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.57 0.54
-
 2-oxoglutarate cosubstrate phenylalanine Rattus norvegicus
2.6.1.57 7.7
-
 L-phenylalanine pH 8.0. 37°C Rattus norvegicus
2.6.1.57 10
-
 L-tryptophan
-
 Rattus norvegicus
2.6.1.57 11.8
-
 L-tyrosine pH 8.0. 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.57 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.57 52000
-
 2 * 52000, SDS-PAGE Rattus norvegicus
2.6.1.57 100000
-
 gel filtration, sucrose density gradient centrifugation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.57 Rattus norvegicus
-
 vitamin B6-deficient strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.57 vitamin B6-deficient strain, ammonium sulfate, DEAE-cellulose, isoelectric focusing, Sephacryl s-200, hydroxylapatite Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.57 small intestine mucosa Rattus norvegicus
-
2.6.1.57 small intestine not in muscle phase Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.57 15.4
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.6.1.57 -20°C, at least 3 weeks, no loss of activity Rattus norvegicus
2.6.1.57 0-4°C, 2 weeks, little loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.57 5-hydroxytryptophan + 2-oxoglutarate 19.5% of the activity with phenylalanine Rattus norvegicus 3-(5-hydroxyindole)-2-oxopropanoate + L-glutamate
-
 ?
2.6.1.57 L-phenylalanine + 2-oxoglutarate best amino acid substrate, specific for 2-oxoglutarate, little or no activity with glyoxylate, pyruvate, 2-oxo-4-methylthiobutyrate and alpha-ketoadipate Rattus norvegicus phenylpyruvate + L-glutamate
-
 r
2.6.1.57 L-tryptophan + 2-oxoglutarate 39.7% of activity with phenylalanine Rattus norvegicus 3-indole-2-oxopropanoate + L-glutamate
-
 ?
2.6.1.57 L-tyrosine + 2-oxoglutarate 23.7% of activity with phenylalanine Rattus norvegicus p-hydroxyphenylpyruvate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.57 dimer 2 * 52000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.57 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.57 55
-
 approx. 30% and 27% loss of activity after 10 min with L-tyrosine and L-phenylalanine, respectively, as subtrates Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.57 8
-
 with phenylalanine Rattus norvegicus