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Literature summary extracted from

  • Kim, H.; Koo, S.H.; Choi, H.J.; Yang, C.H.
    Characterization of yeast geranylgeranyl transferase type I expressed in Escherichia coli (1996), Mol. Cell, 6, 602-608.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.59 alpha-subunit encoded by RAM2 cloned to the pFC vector, which has an chloroamphenicol resistance gene and beta-subunit encoded by CAL1 cloned to the pFlag vector, which has an ampicillin resistance gene, these two recombinant enzymes are cotransformed and expressed in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.59 0.00001
-
 geranylgeranyl diphosphate pH 7.5, 37°C Saccharomyces cerevisiae
2.5.1.59 0.00143
-
 [glutathione S-transferase]-GCVKIKKCVIL pH 7.5, 37°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.59 Mg2+ required, optimal activity of EDTA-treated enzyme at 0.5 mM, Mg2+ probably activates the diphosphate leaving group of enzyme, and is essential for transferring the bound peptide substrate Saccharomyces cerevisiae
2.5.1.59 Zn2+ enzyme contains tightly bound Zn2+ which is required for its catalytic activity Bos taurus
2.5.1.59 Zn2+ requirement, optimal activity of EDTA-treated enzyme at 0.01 mM in presence of 1 mM Mg2+ Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine Saccharomyces cerevisiae
-
 S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine Bos taurus
-
 S-geranylgeranyl-protein + diphosphate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.5.1.59 additional information no enzyme activity in the presence of EDTA Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.59 Bos taurus
-
-
-
2.5.1.59 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.59 recombinant enzyme Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.59 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine
-
 Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine
-
 Bos taurus S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu Bos taurus S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine prenylation, substrates are Rho, Rac, most trimeric G protein gamma subunits Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Bos taurus S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + [glutathione S-transferase]-GCVKIKKCVIL
-
 Saccharomyces cerevisiae diphosphate + ?
-
 ?
2.5.1.59 geranylgeranyl diphosphate + [glutathione S-transferase]-GCVKIKKCVIL
-
 Bos taurus diphosphate + ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.59 6.9 7.3
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.59 8.6
-
 at pH 8.6 no enzyme activity is detected Saccharomyces cerevisiae