Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stirtan, W.G.; Poulter, C.D.
    Yeast protein geranylgeranyltransferase type-I: steady-state kinetics and substrate binding (1997), Biochemistry, 36, 4552-4557.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.59 alpha-subunit encoded by RAM2 and beta-subunit encoded by CDC43 translationally coupled by overlapping the RAM-CDC43 stop-start codons and by locating a ribosome-binding site near the 3' end of RAM2, recombinant enzyme overproduced in Escherichia coli Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.59 1-phosphono-(E,E,E)-geranylgeraniol competitive to geranylgeranyl diphosphate, potent substrate inhibition to dansyl-Gly-Cys-Ile-Ile-Leu Saccharomyces cerevisiae
2.5.1.59 Cys-3-(aminomethyl)benzoic acid-Leu noncompetitive to geranylgeranyl diphosphate, competitive to dansyl-Gly-Cys-Ile-Ile-Leu Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.59 0.00094
-
 geranylgeranyl diphosphate pH 7.5, 30°C Saccharomyces cerevisiae
2.5.1.59 0.0018
-
 dansyl-Gly-Cys-Ile-Ile-Leu pH 7.5, 30°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine Saccharomyces cerevisiae
-
 S-geranylgeranyl-protein + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.59 Saccharomyces cerevisiae
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate an ordered binding mechanism for enzyme where geranylgeranyl diphosphate adds before peptide Saccharomyces cerevisiae
a
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate this enzyme, along with protein farnesyltransferase, EC 2.5.1.58 and protein geranylgeranyltransferase type II, EC 2.5.1.60, constitutes the protein prenyltransferase family of enzymes Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.59 geranylgeranyl diphosphate + dansyl-Gly-Cys-Ile-Ile-Leu
-
 Saccharomyces cerevisiae dansyl-Gly-(S-geranylgeranyl)-Cys-Ile-Ile-Leu + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine
-
 Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine prenylation, substrates are Rho, Rac, most trimeric G protein gamma subunits Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X is Leu or Phe Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.59 0.34
-
 geranylgeranyl diphosphate
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.59 additional information
-
 additional information KI for varied concentrations of dansyl-Gly-Cys-Ile-Ile-Leu Saccharomyces cerevisiae
2.5.1.59 0.0002
-
 1-phosphono-(E,E,E)-geranylgeraniol with respect to geranylgeranyl diphosphate Saccharomyces cerevisiae
2.5.1.59 0.078
-
 Cys-3-(aminomethyl)benzoicacid-Leu with respect to dansyl-Gly-Cys-Ile-Ile-Leu Saccharomyces cerevisiae
2.5.1.59 0.14
-
 Cys-3-(aminomethyl)benzoicacid-Leu with respect to geranylgeranyl diphosphate Saccharomyces cerevisiae