Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhang, F.L.; Moomaw, J.F.; Casey, P.J.
    Properties and kinetic mechanism of recombinant mammalian protein geranylgeranyltransferase type I (1994), J. Biol. Chem., 269, 23465-23470.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.59 coexpression of two subunits of enzyme by Sf9 cells infected with recombinant baculovirus Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.59 3-aza-geranylgeranyl-diphosphate competitive inhibitor with respect to geranylgeranyl diphosphate, non-competitive to Cys-Val-Phe-Leu Rattus norvegicus
2.5.1.59 Cys-Val-Phe-Leu noncompetitive inhibitor with respect to geranylgeranyl diphosphate, 50% inhibition at 0.0001 mM, competitive to Cys-Val-Phe-Leu Rattus norvegicus
2.5.1.59 additional information TKSer-Val-Ile-Leu inactive as competitor Rattus norvegicus
2.5.1.59 Thr-Lys-Cys-Val-Ile-Leu potent competitor, 50% inhibition at 0.001 mM Rattus norvegicus
2.5.1.59 Thr-Lys-Cys-Val-Ile-Met potent competitor, 50% inhibition at 0.008 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.59 0.000003
-
 geranylgeranyl diphosphate pH 7.7, 30°C Rattus norvegicus
2.5.1.59 0.0012
-
 Ras-Cys-Val-Leu-Leu pH 7.7, 30°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.59 Zn2+ enzyme contains a single zinc atom Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.59 Rattus norvegicus
-
 enzyme produced in Sf9cells using baculovirus system
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.59 recombinant enzyme Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate random sequential mechanism Rattus norvegicus
a
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate this enzyme, along with protein farnesyltransferase, EC 2.5.1.58 and protein geranylgeranyltransferase type II, EC 2.5.1.60, constitutes the protein prenyltransferase family of enzymes Rattus norvegicus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.59 0.007
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine prenylation, substrates are Rho, Rac, most trimeric G protein gamma subunits Rattus norvegicus S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X is Leu, Phe or Ser, preferred substrate: Leu Rattus norvegicus S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Rattus norvegicus S-geranylgeranyl-protein + diphosphate
-
 ?
2.5.1.59 geranylgeranyl diphosphate + Ras-Cys-Val-Leu-Leu
-
 Rattus norvegicus diphosphate + Ras-S-geranylgeranyl-Cys-Val-Leu-Leu
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.59 0.02
-
 geranylgeranyl diphosphate
-
 Rattus norvegicus
2.5.1.59 0.02
-
 Ras-Cys-Val-Leu-Leu
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.59 0.000015
-
 3-aza-geranylgeranyl-diphosphate with respect to geranylgeranyl diphosphate Rattus norvegicus
2.5.1.59 0.000022
-
 3-aza-geranylgeranyl-diphosphate with respect to Ras-Cys-Val-Leu-Leu Rattus norvegicus
2.5.1.59 0.00005
-
 Cys-Val-Phe-Leu with respect to Ras-Cys-Val-Leu-Leu Rattus norvegicus
2.5.1.59 0.000065
-
 Cys-Val-Phe-Leu with respect to geranylgeranyl diphosphate Rattus norvegicus